Protective Effect of Taurine on Apoptosis of Spinal Cord Cells in Diabetic Neuropathy Rats
Diabetes mellitus (DM) is a condition characterized by chronic hyperglycemia, which leads to diabetic neuropathy and apoptosis in the spinal cord. Taurine has been found to ameliorate the diabetic neuropathy and control apoptosis in various tissues. However, there are few reports that discuss the direct relationship between spinal cord and anti-apoptotic effect of taurine. In this study, DM was induced in male SD rats with STZ @ 25 mg/Kg of body weight in combination with high fat diet. After 2 weeks, they were divided into four groups as DM: diabetic rats, T1 (0.5%), T2 (1%) and T3 (2%) taurine solution, while control group was non-diabetic rats (no treatment). The results showed that DM increased apoptosis, decreased phosphorylated Akt and Bad. DM decreased expression of Bcl-2 and increased the Bax. Moreover, the release of cytochrome c into cytosol was increased in DM and activation of caspase-3 was also increased. However, taurine reversed all these abnormal changes in a dose dependent manner. Our results suggested the involvement of Akt/Bad signaling pathway and mitochondrial apoptosis pathway in protective effect of taurine against apoptosis in the spinal cord of diabetic rats. Therefore, taurine may be a potential medicine against diabetic neuropathy by controlling apoptosis.
KeywordsDiabetic neuropathy Spinal cord Apoptosis Taurine Anti-apoptosis
Conflict of Interest
It is declared that there is no conflict of interest among the authors.
It is declared that no animal was harmed or hurt except according to ethics.
It is declared that this manuscript is submitted after consent from all authors and all authors are aware of this submission.
- Garchon HJ, Luan JJ, Eloy L, Bédossa P, Bach JF (1994) Genetic analysis of immune dysfunction in non-obese diabetic (NOD) mice: mapping of a susceptibility locus close to the Bcl-2 gene correlates with increased resistance of NOD T cells to apoptosis induction. Eur J Immunol 24(2):380–384CrossRefGoogle Scholar
- Huxtable RJ (2002) Expanding the circle 1975–1999: sulfur biochemistry and insights on the biological functions of taurins Taurine 4. Springer, New York, pp 1–25Google Scholar
- Lin S, Yang J, Wu G, Liu M, Lv Q, Yang Q, Hu J (2013) Inhibitory effects of taurine on STZ-induced apoptosis of pancreatic islet cells Taurine 8. Springer, New York, pp 287–297Google Scholar
- Oja SS, Saransaari P (2007) Pharmacology of taurine. Paper presented at the Proceedings-Western Pharmacology SocietyGoogle Scholar
- Reske-Nielsen E, Lundbaek K, Gregersen G, Harmsen A (1970) Pathological changes in the central and peripheral nervous system of young long-term diabeticsModifications pathologiques dans le système nerveux central et périphérique de sujets jeunes, diabétiques depuis longtempsPathologische Veränderungen am zentralen und peripheren Nervensystem von jüngeren Langzeit-Diabetikern. Diabetologia 6(2):98–103CrossRefGoogle Scholar
- Steenbergen C, Afshari CA, Petranka JG, Collins J, Martin K, Bennett L et al (2003) Alterations in apoptotic signaling in human idiopathic cardiomyopathic hearts in failure. Am J Phys Heart Circ Phys 284(1):H268–H276Google Scholar