Advertisement

Taurine 11 pp 869-874 | Cite as

Protection of Taurine Against Neurotoxicity Induced by Arsenic in Primary Cortical Neurons

  • Fengyuan Piao
  • Xueying Zhou
  • Dunjia Wang
  • Jing Shao
  • Yachen Li
  • Xiaohui Liu
  • Cong Zhang
  • Enjun Zuo
  • Xiaoxia Shi
  • Shuangyue Li
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

Our group previously reported that taurine has a protective capacity on the hippocampus and cerebellum of arsenic (As)-exposed mouse. In the present study, we explore whether taurine demonstrates protection against As toxicity in primary cortical neurons. Primary cortical neurons were exposed to various concentrations of arsenite and cell viability was assessed to confirm the toxicity of As on cortical neurons. The protection of taurine was examined after primary cortical neurons were treating with arsenite and taurine for 24 h. The cell viability was examined by MTT and caspase-3 activity assay. The expression of Bax and Bcl-2 was determined by western blot. The results showed that As exposure reduced cell viability and enhanced the activity of caspase-3, which were markedly inhibited by taurine treatment. The expression of Bax and Bcl-2 were disturbed by As exposure, which were reversed by taurine. These results indicated that taurine expose protective effect on As-exposed primary cortical neurons and its mechanism maybe involved the regulation of Bax/Bcl-2.

Keywords

Taurine Arsenic Neuroprotection Apoptosis 

References

  1. Batista TM, Ribeiro RA, da Silva PM, Camargo RL, Lollo PC, Boschero AC, Carneiro EM (2013) Taurine supplementation improves liver glucose control in normal protein and malnourished mice fed a high-fat diet. Mol Nutr Food Res 57:423–434CrossRefGoogle Scholar
  2. Bhavsar TM, Patel SN, Lau-Cam CA (2010) Protective action of taurine, given as a pretreatment or as a posttreatment, against endotoxin-induced acute lung inflammation in hamsters. J Biomed Sci 17(Suppl 1):S19CrossRefGoogle Scholar
  3. Braun RJ (2012) Mitochondrion-mediated cell death: dissecting yeast apoptosis for a better understanding of neurodegeneration. Front Oncol 2:182CrossRefGoogle Scholar
  4. Carlin DJ, Naujokas MF, Bradham KD, Cowden J, Heacock M, Henry HF, Lee JS, Thomas DJ, Thompson C, Tokar EJ, Waalkes MP, Birnbaum LS, Suk WA (2016) Arsenic and environmental health: state of the science and future research opportunities. Environ Health Perspect 124:890–899CrossRefGoogle Scholar
  5. Higuchi M, Celino FT, Shimizu-Yamaguchi S, Miura C, Miura T (2012) Taurine plays an important role in the protection of spermatogonia from oxidative stress. Amino Acids 43:2359–2369CrossRefGoogle Scholar
  6. Kuo CC, Moon K, Thayer KA, Navas-Acien A (2013) Environmental chemicals and type 2 diabetes: an updated systematic review of the epidemiologic evidence. Curr Diab Rep 13:831–849CrossRefGoogle Scholar
  7. Li S, Yang L, Zhang Y, Zhang C, Shao J, Liu X, Li Y, Piao F (2017) Taurine ameliorates arsenic-induced apoptosis in the Hippocampus of mice through intrinsic pathway. Adv Exp Med Biol 975:183–192CrossRefGoogle Scholar
  8. Men X, Han S, Gao J, Cao G, Zhang L, Yu H, Lu H, Pu J (2010) Taurine protects against lung damage following limb ischemia reperfusion in the rat by attenuating endoplasmic reticulum stress-induced apoptosis. Acta Orthop 81:263–267CrossRefGoogle Scholar
  9. Navas-Acien A, Sharrett AR, Silbergeld EK, Schwartz BS, Nachman KE, Burke TA, Guallar E (2005) Arsenic exposure and cardiovascular disease: a systematic review of the epidemiologic evidence. Am J Epidemiol 162:1037–1049CrossRefGoogle Scholar
  10. Peters BA, Liu X, Hall MN, Ilievski V, Slavkovich V, Siddique AB, Alam S, Islam T, Graziano JH, Gamble MV (2015) Arsenic exposure, inflammation, and renal function in Bangladeshi adults: effect modification by plasma glutathione redox potential. Free Radic Biol Med 85:174–182CrossRefGoogle Scholar
  11. Schuller-Levis GB, Gordon RE, Park E, Pendino KJ, Laskin DL (1995) Taurine protects rat bronchioles from acute ozone-induced lung inflammation and hyperplasia. Exp Lung Res 21:877–888CrossRefGoogle Scholar
  12. Schuller-Levis GB, Gordon RE, Wang C, Park E (2003) Taurine reduces lung inflammation and fibrosis caused by bleomycin. Adv Exp Med Biol 526:395–402CrossRefGoogle Scholar
  13. Sinczuk-Walczak H (2009) Nervous system disorders induced by occupational exposure to arsenic and its inorganic compounds: a literature review. Med Pr 60:519–522PubMedGoogle Scholar
  14. Teng YC, Tai YI, Lee YH, Lin AM (2013) Role of HO-1 in the arsenite-induced neurotoxicity in primary cultured cortical neurons. Mol Neurobiol 48:281–287CrossRefGoogle Scholar
  15. Wang Y, Wu Y, Luo K, Liu Y, Zhou M, Yan S, Shi H, Cai Y (2013) The protective effects of selenium on cadmium-induced oxidative stress and apoptosis via mitochondria pathway in mice kidney. Food Chem Toxicol 58:61–67CrossRefGoogle Scholar
  16. Zhang J, Liu X, Zhao L, Hu S, Li S, Piao F (2013) Subchronic exposure to arsenic disturbed the biogenic amine neurotransmitter level and the mRNA expression of synthetase in mice brains. Neuroscience 241:52–58CrossRefGoogle Scholar
  17. Zhang C, Li S, Sun Y, Dong W, Piao F, Piao Y, Liu S, Guan H, Yu S (2014a) Arsenic downregulates gene expression at the postsynaptic density in mouse cerebellum, including genes responsible for long-term potentiation and depression. Toxicol Lett 228:260–269CrossRefGoogle Scholar
  18. Zhang Z, Liu D, Yi B, Liao Z, Tang L, Yin D, He M (2014b) Taurine supplementation reduces oxidative stress and protects the liver in an iron-overload murine model. Mol Med Rep 10:2255–2262CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Fengyuan Piao
    • 1
  • Xueying Zhou
    • 1
  • Dunjia Wang
    • 1
  • Jing Shao
    • 1
  • Yachen Li
    • 1
  • Xiaohui Liu
    • 1
  • Cong Zhang
    • 2
  • Enjun Zuo
    • 3
  • Xiaoxia Shi
    • 1
  • Shuangyue Li
    • 1
  1. 1.Department of Occupational and Environmental HealthDalian Medical UniversityDalianChina
  2. 2.Department of Nutrition and Food SafetyDalian Medical UniversityDalianChina
  3. 3.College of StomatologyDalian Medical UniversityDalianChina

Personalised recommendations