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Taurine 11 pp 747-754 | Cite as

Taurine Protects Against Arsenic-Induced Apoptosis Via PI3K/Akt Pathway in Primary Cortical Neurons

  • Kaixin Li
  • Dunjia Wang
  • Xueying Zhou
  • Jing Shao
  • Yachen Li
  • Xiaohui Liu
  • Cong Zhang
  • Enjun Zuo
  • Xiaoxia Shi
  • Fengyuan Piao
  • Shuangyue Li
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

Arsenate, a well known toxicant, can induce injury in nerve system via oxidative stress and apoptosis. This study was designed to explore the protective effect of taurine against arsenite-induced neurotoxicity and its related mechanism in primary cortical neurons. The cells were treated with arsenite with or without taurine. Twenty-Four hours later, cell viability was examined using the MTT assay. The activity of caspase-3 was analyzed and the level of Akt and p-Akt were examined by western blot. The results show that taurine treatment significantly attenuates the decrease in cell viability of arsenite-exposed primary cortical neurons. Taurine also reversed the arsenite-induced increase in caspase-3 activity. The decrease in p-Akt levels induced by arsenite exposure was prevented by taurine treatment. Thus, taurine attenuated the effect of arsenite on primary cortical neurons, an effect that may involve the Akt pathway.

Keywords

Taurine Arsenic PI3K/AKT Apoptosis 

Abbreviations

As

arsenic

DMSO

dimethyl sulfoxide

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Kaixin Li
    • 1
  • Dunjia Wang
    • 1
  • Xueying Zhou
    • 1
  • Jing Shao
    • 1
  • Yachen Li
    • 1
  • Xiaohui Liu
    • 1
  • Cong Zhang
    • 2
  • Enjun Zuo
    • 3
  • Xiaoxia Shi
    • 1
  • Fengyuan Piao
    • 1
  • Shuangyue Li
    • 1
  1. 1.Department of Occupational and Environmental HealthDalian Medical UniversityDalianChina
  2. 2.Department of Nutrition and Food SafetyDalian Medical UniversityDalianChina
  3. 3.College of StomatologyDalian Medical UniversityDalianChina

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