Taurine 11 pp 739-746 | Cite as

Protective Effect of Taurine on Paraquat-Induced Lung Epithelial Cell Injury

  • Shuangxing Li
  • Jinhua Wang
  • Bin Kai Wei
  • Guangtao Dong
  • Xiujie Wang
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)


The herbicide Paraquat induce oxidative stress-mediated lung injury. Taurine is a well-known antioxidant. This study was designed to explore the effect of taurine on paraquat-induced injury and its related mechanism in A549 cells. The cells were pretreated with various concentrations of taurine for 30 min prior to paraquat exposure. 24 h later, cell viability was examined by the MTT assay. The level of glutathione (GSH) and the activity of glutathione peroxidase (GPx) were analyzed. The results show that taurine treatment significantly attenuates the decrease in cell viability mediated by paraquat in A549 cells. Taurine also reversed paraquat-induced disturbances in GSH content and GPx activity. Taurine exerts protection against paraquat-mediated A549 cell toxicity likely through modulation of oxidative stress.


Taurine Paraquat Oxidative stress Lung injury 





Glutathione peroxidase


dimethyl sulfoxide


5,5′-dithiobis-2-nitrobenzoic acid


  1. Bai Y, Wang Y, Liu M, Gu YH, Jiang B, Wu X, Wang HL (2017) Suppression of nuclear factor erythroid2related factor 2mediated antioxidative defense in the lung injury induced by chronic exposure to methamphetamine in rats. Mol Med Rep 15:3135–3142CrossRefGoogle Scholar
  2. Batista TM, Ribeiro RA, da Silva PM, Camargo RL, Lollo PC, Boschero AC, Carneiro EM (2013) Taurine supplementation improves liver glucose control in normal protein and malnourished mice fed a high-fat diet. Mol Nutr Food Res 57:423–434CrossRefGoogle Scholar
  3. Bhavsar TM, Patel SN, Lau-Cam CA (2010) Protective action of taurine, given as a pretreatment or as a posttreatment, against endotoxin-induced acute lung inflammation in hamsters. J Biomed Sci 17(Suppl 1):S19CrossRefGoogle Scholar
  4. Budhram R, Pandya KG, Lau-Cam CA (2013) Protection by taurine and thiotaurine against biochemical and cellular alterations induced by diabetes in a rat model. Adv Exp Med Biol 775:321–343CrossRefGoogle Scholar
  5. Das J, Ghosh J, Manna P, Sinha M, Sil PC (2009) Taurine protects rat testes against NaAsO(2)-induced oxidative stress and apoptosis via mitochondrial dependent and independent pathways. Toxicol Lett 187:201–210CrossRefGoogle Scholar
  6. Elenga N, Merlin C, Le Guern R, Kom-Tchameni R, Ducrot YM, Pradier M, Ntab B, Dinh-Van KA, Sobesky M, Mathieu D, Dueymes JM, Egmann G, Kallel H, Mathieu-Nolf M (2018) Clinical features and prognosis of paraquat poisoning in French Guiana: a review of 62 cases. Medicine (Baltimore) 97:e9621CrossRefGoogle Scholar
  7. Gao S, Duan X, Wang X, Dong D, Liu D, Li X, Sun G, Li B (2013) Curcumin attenuates arsenic-induced hepatic injuries and oxidative stress in experimental mice through activation of Nrf2 pathway, promotion of arsenic methylation and urinary excretion. Food Chem Toxicol 59:739–747CrossRefGoogle Scholar
  8. Han YY, Shen P, Chang WX (2015) Involvement of epithelial-to-mesenchymal transition and associated transforming growth factor-beta/Smad signaling in paraquat-induced pulmonary fibrosis. Mol Med Rep 12:7979–7984CrossRefGoogle Scholar
  9. Higuchi M, Celino FT, Shimizu-Yamaguchi S, Miura C, Miura T (2012) Taurine plays an important role in the protection of spermatogonia from oxidative stress. Amino Acids 43:2359–2369CrossRefGoogle Scholar
  10. Ikpesu TO (2015) Assessment of occurrence and concentrations of paraquat dichloride in water, sediments and fish from Warri River Basin, Niger Delta, Nigeria. Environ Sci Pollut Res Int 22:8517–8525CrossRefGoogle Scholar
  11. Kim YS, Zerin T, Song HY (2013) Antioxidant action of ellagic acid ameliorates paraquat-induced A549 cytotoxicity. Biol Pharm Bull 36:609–615CrossRefGoogle Scholar
  12. Lee SH, Lee KS, Ahn JM, Kim SH, Hong SY (1995) Paraquat poisoning of the lung: thin-section CT findings. Radiology 195:271–274CrossRefGoogle Scholar
  13. Lee SK, Ameno K, In SW, Yang JY, Kim KU, Koo KS, Yoo YC, Ameno S, Ijiri I (1999) Levels of paraquat in fatal intoxications. Int J Legal Med 112:198–200CrossRefGoogle Scholar
  14. Lin JL, Lin-Tan DT, Chen KH, Huang WH, Hsu CW, Hsu HH, Yen TH (2011) Improved survival in severe paraquat poisoning with repeated pulse therapy of cyclophosphamide and steroids. Intensive Care Med 37:1006–1013CrossRefGoogle Scholar
  15. Men X, Han S, Gao J, Cao G, Zhang L, Yu H, Lu H, Pu J (2010) Taurine protects against lung damage following limb ischemia reperfusion in the rat by attenuating endoplasmic reticulum stress-induced apoptosis. Acta Orthop 81:263–267CrossRefGoogle Scholar
  16. Meng XX, Wang RL, Gao S, Xie H, Tan JT, Qian YB (2013) Effect of ulinastatin on paraquat-induced-oxidative stress in human type II alveolar epithelial cells. World J Emerg Med 4:133–137CrossRefGoogle Scholar
  17. Mershiba SD, Dassprakash MV, Saraswathy SD (2013) Protective effect of naringenin on hepatic and renal dysfunction and oxidative stress in arsenic intoxicated rats. Mol Biol Rep 40:3681–3691CrossRefGoogle Scholar
  18. Mitsopoulos P, Suntres ZE (2010) Cytotoxicity and gene array analysis of alveolar epithelial A549 cells exposed to paraquat. Chem Biol Interact 188:427–436CrossRefGoogle Scholar
  19. Schuller-Levis GB, Gordon RE, Park E, Pendino KJ, Laskin DL (1995) Taurine protects rat bronchioles from acute ozone-induced lung inflammation and hyperplasia. Exp Lung Res 21:877–888CrossRefGoogle Scholar
  20. Schuller-Levis GB, Gordon RE, Wang C, Park E (2003) Taurine reduces lung inflammation and fibrosis caused by bleomycin. Adv Exp Med Biol 526:395–402CrossRefGoogle Scholar
  21. Sun X, Gu J, Chi M, Li M, Lei S, Wang G (2014) Activation of PI3K-Akt through taurine is critical for propofol to protect rat cardiomyocytes from doxorubicin-induced toxicity. Can J Physiol Pharmacol 92:155–161CrossRefGoogle Scholar
  22. Toblli JE, Cao G, Giani JF, Dominici FP, Angerosa M (2017) Markers of oxidative/nitrosative stress and inflammation in lung tissue of rats exposed to different intravenous iron compounds. Drug Des Devel Ther 11:2251–2263CrossRefGoogle Scholar
  23. Vadivelan M, Chellappan A, Suryanarayana BS (2014) The ‘golden hour’ in paraquat poisoning. Toxicol Int 21:339–340CrossRefGoogle Scholar
  24. Wei TY, Yen TH, Cheng CM (2018) Point-of-care testing in the early diagnosis of acute pesticide intoxication: the example of paraquat. Biomicrofluidics 12:011501CrossRefGoogle Scholar
  25. Yamada A, Aki T, Unuma K, Funakoshi T, Uemura K (2015) Paraquat induces epithelial-mesenchymal transition-like cellular response resulting in fibrogenesis and the prevention of apoptosis in human pulmonary epithelial cells. PLoS One 10:e0120192CrossRefGoogle Scholar
  26. Zerin T, Kim YS, Hong SY, Song HY (2012) Protective effect of methylprednisolone on paraquat-induced A549 cell cytotoxicity via induction of efflux transporter, P-glycoprotein expression. Toxicol Lett 208:101–107CrossRefGoogle Scholar
  27. Zhang Z, Liu D, Yi B, Liao Z, Tang L, Yin D, He M (2014) Taurine supplementation reduces oxidative stress and protects the liver in an iron-overload murine model. Mol Med Rep 10:2255–2262CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Shuangxing Li
    • 1
  • Jinhua Wang
    • 2
  • Bin Kai Wei
    • 2
  • Guangtao Dong
    • 1
  • Xiujie Wang
    • 1
  1. 1.Department of Emergency SurgeryThe First Affiliated Hospital of Harbin Medical UniversityHarbinChina
  2. 2.Clinical Medicine (5+3)Dalian Medical UniversityDalianChina

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