A Hepatoprotective Effect of a Hot Water Extract from Loliolus beka Gray Meat Against H2O2-Induced Oxidative Damage in Hepatocytes
Here, we investigated the hepatoprotective effect of a hot water extract from Loliolus beka gray meat (LBMH) containing plentiful taurine in H2O2-induced oxidative stress in hepatocytes. LBMH potently scavenged the 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and exhibited the good reducing power and the oxygen radical absorbance capacity (ORAC) value. Also, LBMH improved the cell viability against H2O2-induced hepatic damage in cultured hepatocytes by reducing intracellular reactive oxygen species (ROS) production. In addition, LBMH inhibited apoptosis via a reduction in sub-G1 cell population, as well as inhibition of apoptotic body formation from H2O2-induced oxidative damage in hepatocytes. Moreover, LBMH regulated the expression levels of Bax, a pro-apoptotic molecule and Bcl-2, an anti-apoptotic molecule in H2O2-treated hepatocytes. Additionally, pre-treatment with LBMH increased the expression of heme oxygenase 1 (HO-1), which is a hepatoprotective enzyme, by activating the nuclear factor erythroid 2-related factor 2 (Nrf2) in H2O2-treated hepatocytes. Taken together, LBMH may be useful as a food ingredient for treatment of liver disease by regulating the Nrf2/HO-1 signal pathway.
KeywordsLoliolus beka gray meat Nrf2/HO-1 signal pathway Antioxidant activity ROS Apoptosis
This research was supported by Basic Science Research Program through the National Research Foundation of Kore(NRF) funded by the Ministry of Education (2018016523).
- Han EJ, Um JH, Kim EA, Lee W, Kang N, Oh JY, Ahn G (2017) Protective effects of a water extracts prepared from Loliolus beka gray meat against H2O2-induced oxidative stress in Chang liver cells and zebrafish embryo model, Advances in experimental medicine and biology. Springer, Dordrecht, pp 585–601Google Scholar
- Je JY, Park PJ, Kim SK (2005) Antioxidant activity of a peptide isolated from Alaska pollack (Theragra chalcogramma) frame protein hydrolysate. IFRJ 38:45–45Google Scholar
- Li S, Yang L, Dong G, Wang X (2017) Taurine protects mouse liver against arsenic-induced apoptosis through JNK pathway. Adv Exp Med Biol:855–862Google Scholar
- Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods. Impact on human health. PMC 4:118Google Scholar
- Poprac P, Jomova K, Simunkova M, Kollar V, Rhodes CJ, Valko M (2017) Targeting free radicals in oxidative stress-related human diseases. Trends Cell Biol 38:592–607Google Scholar
- Rajapakse N, Mendis E, Jung WK, Je JY, Kim SK (2005) Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties. IFRJ 38:175–182Google Scholar
- Siahpoosh A, Alikhani K (2016) Evaluation of antioxidant capacity and free radical scavenging activities of pepsin extract of cuttlefish (Sepia pharaonis) form persian gulf. Ind J Tra Kno:604–610Google Scholar
- Um JH, Kim EA, Lee W, Kang N, Han EJ, Oh JY, Ahn G (2017) Protective effects of an enzymatic hydrolysate from Octopus ocellatus meat against hydrogen peroxide-induced oxidative stress in Chang liver cells and zebrafish embryo. Adv Exp Med Biol:603–620Google Scholar
- Wu HC, Chen HM, Shiau CY (2003) Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). IFRJ 36:949–957Google Scholar
- Wu G, Yang J, Lv H, Jing W, Zhou J, Feng Y, Hu J (2018) Taurine prevents ethanol-induced apoptosis mediated by mitochondrial or death receptor pathways in liver cells. Amino acids:1–13Google Scholar
- You L, Zhao M, Cui C, Zhao H, Yang B (2009) Effect of degree of hydrolysis on the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates. IFSET 10:235–240Google Scholar