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Taurine 11 pp 451-462 | Cite as

Taurine Prevented Hypoxia Induced Chicken Cardiomyocyte Apoptosis Through the Inhibition of Mitochondrial Pathway Activated by Calpain-1

  • Qiufeng Lv
  • Jiancheng Yang
  • Yue Wang
  • Mei Liu
  • Ying Feng
  • Gaofeng Wu
  • Shumei Lin
  • Qunhui Yang
  • Jianmin Hu
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)

Abstract

Objective To determine whether taurine has protective effects on chicken myocardial apoptosis induced by hypoxic condition through inhibiting calpain-1 derived mitochondrial apoptotic pathway. Methods Chicken primary embryonic myocardial cells were isolated and cultured at 37 °C under a 5% CO2 atmosphere. Firstly the optimum concentration of taurine or PD150606 was chosen by detecting the cell viability. Chicken cardiomyocytes were cultured in 95% N2-5% CO2 atmosphere for 12 h to produce hypoxic conditions. Before hypoxic treatment, 10 mM taurine and 10 uM PD150606 (a specific calpains inhibitor) were added separately or together. The cell apoptosis was detected by acridine orange/ethidium bromide (AO/EB) double staining. Western blotting was used to determine the protein expressions of calpain-1, cytochrome c, Bcl-2, procaspase-9 and procaspase-3 in the cardiomyocytes. Results Taurine administration effectively attenuated the myocardial apoptosis under hypoxic condition, reduced the calpain-1 protein level. In addition, pre-treated taurine could up-regulate the protein expressions of Bcl-2 and procaspase-3 in hypoxic myocardial cells, down-regulate protein expression levels of cytochrome c and procaspase-9. Moreover, taurine exhibited same inhibition effect as PD150606 on the cell apoptosis and proteins express under hypoxic condition. Conclusions Taurine could attenuate the chicken cardiomyocyte apoptosis impaired by hypoxia through inhibiting calpian-1-derived mitochondrial apoptotic pathway in vitro.

Keywords

Taurine Apoptosis Calpain-1 Mitochondrial apoptotic pathway Chicken primary myocardial cells 

Notes

Acknowledgements

This study was supported by a grant from the National Natural Science Foundation (No. 31502026) of china.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Qiufeng Lv
    • 1
  • Jiancheng Yang
    • 1
  • Yue Wang
    • 1
  • Mei Liu
    • 1
  • Ying Feng
    • 1
  • Gaofeng Wu
    • 1
  • Shumei Lin
    • 1
  • Qunhui Yang
    • 1
  • Jianmin Hu
    • 1
  1. 1.Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science & Veterinary MedicineShenyang Agricultural UniversityShenyangPeople’s Republic of China

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