Taurine 11 pp 369-380 | Cite as

Modification by Ethanol and Taurine, Singly and in Combination, of Changes in Indices of Renal Dysfunction Caused by Diabetes in Rats

  • Sanket N. Patel
  • Cesar A. Lau-Cam
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)


The present study was carried out in diabetic rats to examine the effects of ethanol (EtOH) and taurine (TAU), singly and in combination, in reducing the changes of laboratory test values indicating renal dysfunction. For this purpose, male Sprague-Dawley rats, 250–280 g in weight and in groups of 6, were made diabetic with a single, 60 mg/kg intraperitoneal dose of streptozotocin in 10 mM citrate buffer pH 4.5. On day 15 and for the remaining 14 days of the study, the diabetic rats (a) started to drink 5% EtOH in place of water, (b) received a single daily 2.4 mM/kg oral dose of TAU or (c) were allowed to drink 5% EtOH after receiving a dose of TAU. Starting from day 28 and ending on day 29, a 24 h urine sample was collected, its volume was measured, and then used to measure glucose (GLC), total protein (TP) and electrolytes (Na+, K+, Ca++, Mg++). Blood samples collected immediately thereafter via cardiac puncture were processed for the plasma fractions which were analyzed for their creatinine (CRT) and urea nitrogen (UN) contents. In comparison to normal (control) rats, diabetic ones showed a higher output of urine (+5.6-fold), a massive increase in plasma GLC (+473%), passed more GLC (+73.8-fold) and TP (+8.2-fold) in the urine, showed higher plasma CRT (+241%) and UN (+74%) levels, a lower plasma UN/CRT ratio (−47%) and a greater output of electrolytes in the urine (by at least twofold). By themselves both EtOH and TAU were found to markedly lower the effects of diabetes, with EtOH generally appearing more effective than TAU. However, the concurrent availability of EtOH and TAU was found to be more protective than either treatment alone.


Diabetes Rats Ethanol Taurine Plasma Urine Glucose Creatinine Urea nitrogen Total proteins Electrolytes 

















Urea nitrogen






Total protein


  1. Alicic RZ, Rooney MT, Tuttle KR (2017) Diabetic kidney disease: challenges, progress, and possibilities. Clin J Am Soc Nephrol 12:2032–2045CrossRefGoogle Scholar
  2. Arakawa K, Ishihara T, Oku A, Nawano M, Ueta K, Kitamura K, Matsumoto M, Saito A (2001) Improved diabetic syndrome in C57BL/KsJ-db/db mice by oral administration of the Na+-glucose cotransporter inhibitor T-1095. Brit J Pharmacol 132:578–586CrossRefGoogle Scholar
  3. Araki S, Haneda M, Koya D, Kondo K, Tanaka S, Arima H, Kume S, Nakazawa J, Chin-Kanasaki M et al (2015) Urinary potassium excretion and renal and cardiovascular complications in patients with type 2 diabetes and normal renal function. Clin J Am Soc Nephrol 10:2152–2158CrossRefGoogle Scholar
  4. Arikawe AP, Udenze IC, Akinwolere MF, Ogunsola AO, Oghogholosu RT (2012) Effects of streptozotocin, fructose and sucrose-induced insulin resistance on plasma and urinary electrolytes in male Sprague-Dawley rats. Nig Q J Hosp Med 22:224–230PubMedGoogle Scholar
  5. Budhram R, Pandya KG, Lau-Cam CA (2013) Protection by taurine and thiotaurine against biochemical and cellular alterations induced by diabetes in a rat model. Adv Exp Med Biol 775:321–343CrossRefGoogle Scholar
  6. Cheungpasitporn W, Thongprayoon C, Kittanamongkolchai W, Brabec BA, O’Corragain OA, Edmonds PJ, Erickson SB (2015) High alcohol consumption and the risk of renal damage: a systematic review and meta-analysis. QJM 108:539–548CrossRefGoogle Scholar
  7. Chow F, Ozols E, Nikolic-Paterson DJ, Atkinas RC, Tesch GH (2004) Macrophages in mouse type 2 diabetic nephropathy: correlation with diabetic state and progressive renal injury. Kidney Int 65:116–128CrossRefGoogle Scholar
  8. Chung FM, Yang YH, Shieh TY, Shin SJ, Tsai JC, Lee YJ (2005) Effect of alcohol consumption on estimated glomerular filtration rate and creatinine clearance rate. Nephrol Dial Transplant 20:1610–1616CrossRefGoogle Scholar
  9. Das J, Sil PC (2012) Taurine ameliorates alloxan-induced diabetic renal injury, oxidative stress-related signaling pathways and apoptosis in rats. Amino Acids 43:1509–1523CrossRefGoogle Scholar
  10. Furuya DT, Binsack R, Machado UF (2003) Low ethanol consumption increases insulin sensitivity in Wistar rats. Braz J Med Biol Res 36:125–130CrossRefGoogle Scholar
  11. Ha H, Yu MR, Kim KH (1999) Melatonin and taurine reduce early glomerulopathy in diabetic rats. Free Radic Biol Med 26:944–950CrossRefGoogle Scholar
  12. He L, Marecki JC, Serrero G, Simmen FA, Ronis MJ, Badger TM (2007) Dose-dependent effects of alcohol on insulin signaling: partial explanation for biphasic alcohol impact on human health. Mol Endocrinol 21:2541–2550CrossRefGoogle Scholar
  13. Hosten AO (1990) BUN and creatinine. In: Walker HK, Hall WD, Hurst JW (eds) Clinical methods: the history, physical, and laboratory examinations, 3rd edn. Butterworths, Boston, pp 874–878Google Scholar
  14. Hsu Y-H, Pai H-C, Chang Y-M, Liu W-H, Hsu C-C (2013) Alcohol consumption is inversely associated with stage 3 chronic kidney disease in middle-aged Taiwanese men. BMC Nephrol 14:254CrossRefGoogle Scholar
  15. Koh JH, Lee ES, Hyun M, Kim HM, Choi YJ, Lee EY, Yada D, Chung CH (2014) Taurine alleviates the progression of diabetic nephropathy in type 2 diabetic rat model. Int J Endocrinol 2014:397307CrossRefGoogle Scholar
  16. Koppes LL, Dekker JM, Hendriks HF, Bouter LM, Heine RJ (2005) Moderate alcohol consumption lowers the risk of type 2 diabetes: a meta-analysis of prospective observational studies. Diabetes Care 28:719–725CrossRefGoogle Scholar
  17. Latchoumycandane C, Nagy LE, McIntyre TM (2014) Chronic ethanol ingestion induces oxidative kidney injury through taurine-inhibitable inflammation. Free Radic Biol Med 69:403–416CrossRefGoogle Scholar
  18. Lin S, Yang J, Wu G, Liu M, Luan X, Ly Q, Zhao H, Hu J (2010) Preventive effect of taurine on experimental type II diabetic nephropathy. J Biomed Sci 17(Suppl 1):S46CrossRefGoogle Scholar
  19. Ma Z, Gao Y, Ma H, Zheng L, Dai B, Miao J, Zhang Y (2016) Effects of taurine and housing density on renal function in laying hens. J Zhejiang Univ Sci B 17:952–964CrossRefGoogle Scholar
  20. Manoeuvrier G, Bach-Ngohou K, Batard E, Masson D, Trewick D (2017) Diagnostic performance of serum blood urea nitrogen to creatinine ratio for distinguishing prerenal from intrinsic acute kidney injury in the emergency department. BMC Nephrol 18:173CrossRefGoogle Scholar
  21. McCarthy ET, Zhou J, Eckert R, Genochio D, Sharma R, Oni O, De A, Srivastava T, Sharma R, Savin VJ, Sharma M (2015) Ethanol at low concentrations protects glomerular podocytes through alcohol dehydrogenase and 20-HETE. Prostaglandins Other Lipid Mediat 0:88–98CrossRefGoogle Scholar
  22. Pandya KG, Buhdram R, Clark CJ, Lau-Cam CA (2015) Taurine can enhance he protective acions of metformin against diabetes-induced alterations adversely affecting renal function. Adv Exp Med Biol 803:227–250CrossRefGoogle Scholar
  23. Reynolds K, Gu D, Chen J, Tang X, Yau CL, Yu L, Chen C-S, Wu X, Hamm LL, He J (2008) Alcohol consumption and the risk of end-stage renal disease among Chinese men. Kidney Int 73:870–876CrossRefGoogle Scholar
  24. Savdie E, Grosslight GM, Adena MA (1984) Relation of alcohol and cigarette consumption to blood pressure and serum creatinine levels. J Chronic Dis 37:617–623CrossRefGoogle Scholar
  25. Schaeffner E, Ritz E (2012) Alcohol and kidney damage: a Janus-faced relationship. Kidney Int 81:816–818CrossRefGoogle Scholar
  26. Shanmugam KR, Mallikarjuna K, Reddy KS (2011) Effect of alcohol on blood glucose and antioxidant enzymes in the liver and kidney of diabetic rats. Indian J Pharmacol 43:330–335CrossRefGoogle Scholar
  27. Smith SA, Lister CA, Toseland CD, Buckingham RE (2000) Rosiglitazone prevents the onset of hyperglycaemia and proteinuria in the Zucker diabetic fatty rat. Diabetes Obes Metab 2:363–372CrossRefGoogle Scholar
  28. Trachtman H, Del Pizzo R, Futterweit S, Levine D, Rao PS, Valderrama E, Sturman JA (1992) Taurine attenuates renal disease in chronic puromycin aminonucleoside nephropathy. Am J Phys 262:F117–F123Google Scholar
  29. Trachtman H, Futterweit S, Maesaka J, Ma C, Valderrama E, Fuchs A, Tarectecan A, Rao PS, Sturman JA, Boles TH et al (1995) Taurine ameliorates chronic streptozotocin-induced diabetic nephropathy in rats. Am J Physiol - Renal Physiol 269:F429–F438CrossRefGoogle Scholar
  30. United States Department of Agriculture (2013) Animal welfare act and animal welfare regulations. Animal Care Blue Book, Washington, DCGoogle Scholar
  31. Yamagata K, Ishida K, Sairenchi T, Takahashi H, Ohba S, Shiigai T, Narita M, Koyama A (2007) Risk factors for chronic kidney disease in a community-based population: a 10-year follow-up study. Kidney Int 71:159–166CrossRefGoogle Scholar
  32. Ying W, Qin W, Gao Y (2018) Urine glucose levels are disordered before blood glucose level increase was observed in Zucker diabetic fatty rats. Sci China Life Sci 61:844–848CrossRefGoogle Scholar
  33. Zhang S, Xu H, Yu X, Wu Y, Sui D (2017) Metformin ameliorates diabetic nephropathy in a rat model of low-dose streptozotocin-induced diabetes. Exp Therap Med 14:383–390CrossRefGoogle Scholar
  34. Ziyadeh FN, Goldfarb S (1991) The renal tubulointerstitium in diabetes mellitus. Kidney Int 39:464–475CrossRefGoogle Scholar
  35. Zoccali C, Kramer A, Jager KJ (2009) Chronic kidney disease and end-stage renal disease – a review produced to contribute to the report ‘the status of health in the European Union: towards a healthier Europe’. NDT Plus 3:213–224PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Sanket N. Patel
    • 1
  • Cesar A. Lau-Cam
    • 1
  1. 1.Department of Pharmaceutical Sciences, College of Pharmacy and Health ProfessionsSt. John’s UniversityJamaicaUSA

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