Effect of Aging on Taurine Transporter (TauT) Expression in the Mouse Brain Cortex

  • Manoj Kumar Neog
  • Hyunju Chung
  • Min Joo Jang
  • Dong Jin Kim
  • Sang Ho Lee
  • Kyoung Soo KimEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)


Taurine content in an older brain is decreased compared to a younger brain and is associated with cognitive deficits. It is not yet known whether the decrease in taurine content is associated with decreased expression of taurine inflow mediating transporters during the aging process. In this study, we investigated whether aging affects taurine transporter and glycine transporter 1 expression in the brain cortex of the mouse. Taurine and glycine transporter expression was compared in the brain cortex of C57BL/6 mice at different ages (2, 12, and 24 months) and to age-matched NLRP3 inflammasome knockout mice. In wild type mice, taurine transporter (TauT) expression in the brain cortex of 12- or 24-month-old mice did not significantly differ from TauT expression in 2-month-old mice. Moreover, TauT expression in the brain cortex of 12- or 24-month-old mice did not significantly differ from age-matched NLRP3 KO mice. This result indirectly suggests that TauT expression may be not affected by aging or age-induced inflammation. In addition, glycine transporter expression was similar to the TauT expression pattern. In conclusion, aging and age-related inflammation might not significantly affect taurine and glycine transporter expression in aged mice. Thus, the decrease of taurine content in an older brain, which is associated with cognitive deficits, may not be significantly related to altered taurine and glycine transporter expression.


Taurine Transporter (TauT) Glycine Transporter (GLYT) NLRP3 Age-related diseases 



This research was supported by a grant from the Korean Health Technology R&D Project through the Korean Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C0658). This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (grant number: 2017R1A1B03031409).


  1. Beckman KB, Ames BN (1998) The free radical theory of aging matures. Physiol Rev 78:547–581CrossRefGoogle Scholar
  2. Benrabh H, Bourre JM, Lefauconnier JM (1995) Taurine transport at the blood-brain barrier: an in vivo brain perfusion study. Brain Res 692:57–65CrossRefGoogle Scholar
  3. Dubroqua S, Singer P, Boison D, Feldon J, Mohler H, Yee BK (2010) Impacts of forebrain neuronal glycine transporter 1 disruption in the senescent brain: evidence for age-dependent phenotypes in Pavlovian learning. Behav Neurosci 124:839–850CrossRefGoogle Scholar
  4. Dunn OJ (1964) Multiple comparisons using rank sums. Technometrics 6:11CrossRefGoogle Scholar
  5. Ferguson CA, Audesirk G (1990) Effects of DDT and permethrin on neurite growth in cultured neurons of chick embryo brain and Lymnaea stagnalis. Toxicol In Vitro 4:23–30CrossRefGoogle Scholar
  6. Franceschi C, Bonafe M, Valensin S, Olivieri F, De Luca M, Ottaviani E, De Benedictis G (2000) Inflamm-aging. An evolutionary perspective on immunosenescence. Ann N Y Acad Sci 908:244–254CrossRefGoogle Scholar
  7. Gebara E, Udry F, Sultan S, Toni N (2015) Taurine increases hippocampal neurogenesis in aging mice. Stem Cell Res 14:369–379CrossRefGoogle Scholar
  8. Harada CN, Natelson Love MC, Triebel KL (2013) Normal cognitive aging. Clin Geriatr Med 29:737–752CrossRefGoogle Scholar
  9. Harman D (1972) The biologic clock: the mitochondria? J Am Geriatr Soc 20:145–147CrossRefGoogle Scholar
  10. Hayflick L (2000a) The future of ageing. Nature 408:267–269CrossRefGoogle Scholar
  11. Hayflick L (2000b) New approaches to old age. Nature 403:365CrossRefGoogle Scholar
  12. Jung MK, Kim KY, Lee NY, Kang YS, Hwang YJ, Kim Y, Sung JJ, McKee A, Kowall N, Lee J, Ryu H (2013) Expression of taurine transporter (TauT) is modulated by heat shock factor 1 (HSF1) in motor neurons of ALS. Mol Neurobiol 47:699–710CrossRefGoogle Scholar
  13. Kim HY, Kim HV, Yoon JH, Kang BR, Cho SM, Lee S, Kim JY, Kim JW, Cho Y, Woo J, Kim Y (2014) Taurine in drinking water recovers learning and memory in the adult APP/PS1 mouse model of Alzheimer’s disease. Sci Rep 4:7467CrossRefGoogle Scholar
  14. Pow DV, Sullivan R, Reye P, Hermanussen S (2002) Localization of taurine transporters, taurine, and (3)H taurine accumulation in the rat retina, pituitary, and brain. Glia 37:153–168CrossRefGoogle Scholar
  15. Tosato M, Zamboni V, Ferrini A, Cesari M (2007) The aging process and potential interventions to extend life expectancy. Clin Interv Aging 2:401–412PubMedPubMedCentralGoogle Scholar
  16. Toyoda A, Koike H, Nishihata K, Iio W, Goto T (2015) Effects of chronic taurine administration on gene expression, protein translation and phosphorylation in the rat hippocampus. Adv Exp Med Biol 803:473–480CrossRefGoogle Scholar
  17. Wang Z, Meng S, Cao L, Chen Y, Zuo Z, Peng S (2018) Critical role of NLRP3-caspase-1 pathway in age-dependent isoflurane-induced microglial inflammatory response and cognitive impairment. J Neuroinflammation 15:109CrossRefGoogle Scholar
  18. Wenting L, Ping L, Haitao J, Meng Q, Xiaofei R (2014) Therapeutic effect of taurine against aluminum-induced impairment on learning, memory and brain neurotransmitters in rats. Neurol Sci 35:1579–1584CrossRefGoogle Scholar
  19. Wu JY (1982) Purification and characterization of cysteic acid and cysteine sulfinic acid decarboxylase and L-glutamate decarboxylase from bovine brain. Proc Natl Acad Sci U S A 79:4270–4274CrossRefGoogle Scholar
  20. Zafra F, Gimenez C (2008) Glycine transporters and synaptic function. IUBMB Life 60:810–817CrossRefGoogle Scholar
  21. Zhang L, Yuan Y, Tong Q, Jiang S, Xu Q, Ding J, Zhang L, Zhang R, Zhang K (2016a) Reduced plasma taurine level in Parkinson’s disease: association with motor severity and levodopa treatment. Int J Neurosci 126:630–636PubMedGoogle Scholar
  22. Zhang Y, Li D, Li H, Hou D, Hou J (2016b) Taurine pretreatment prevents isoflurane-induced cognitive impairment by inhibiting ER stress-mediated activation of apoptosis pathways in the hippocampus in aged rats. Neurochem Res 41:2517–2525CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Manoj Kumar Neog
    • 1
  • Hyunju Chung
    • 2
  • Min Joo Jang
    • 3
  • Dong Jin Kim
    • 4
  • Sang Ho Lee
    • 4
  • Kyoung Soo Kim
    • 3
    • 5
    Email author
  1. 1.School of Bio Sciences and TechnologyVIT UniversityVelloreIndia
  2. 2.Core Research Laboratory Clinical Research Institute Kyung Hee University Hospital at GangdongKyung Hee University School of MedicineSeoulSouth Korea
  3. 3.Department of Clinical Pharmacology and Therapeutics, School of MedicineKyung Hee UniversitySeoulSouth Korea
  4. 4.Division of Nephrology, Department of Internal Medicine, Kyung Hee University Hospital at GangdongKyung Hee UniversitySeoulSouth Korea
  5. 5.East-West Bone & Joint Disease Research InstituteKyung Hee University Hospital at GangdongGangdong-gu, SeoulSouth Korea

Personalised recommendations