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Anticancer Properties of Marine Peptides/Toxins Using Zebrafish Model

  • Saravanan Ramachandran
  • Senthilkumar Rajagopal
Chapter

Abstract

The biological diversity of the marine atmosphere is immense and therefore is an astonishing resource for the anticancer and new drug discovery. Modern technological and methodological developments in isolation, purification and structure elucidation of toxins and cyclic peptides have been evaluated for various novel anticancer screening. In this chapter, the cytotoxicity of the PSG toxin against breast cancer cell lines is examined using the MTT assay. The PSG toxin of selected cuttlefish exhibits highest cytotoxicity against the MCF-7 breast cancer cells (IC50–10.64 μM). Zebrafish xenograft is the topmost model for drug screening in the arena of cancer research and preclinical study. The zebrafish xenotransplantation model is a distinctive and less cost-effective tool for the investigation of cancer cell biology.

Keywords

Marine toxins Peptides Breast cancer MTT assay Zebrafish 

Notes

Acknowledgement

The author gratefully acknowledges the Department of Biotechnology, Ministry of Science and Technology, Government of India (BT/PR15676/AAQ/03/794/2016).

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Saravanan Ramachandran
    • 1
  • Senthilkumar Rajagopal
    • 2
  1. 1.Native Medicine and Marine Pharmacology Laboratory, Faculty of Allied Health SciencesChettinad Hospital and Research Institute, Chettinad Academy of Research and Education (Deemed to be a University)KelambakkamIndia
  2. 2.Department of BiochemistryRayalaseema UniversityKurnoolIndia

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