Historical Investigations and Advances in Flow Cytometry-Based Tests in Paroxysmal Nocturnal Hemoglobinuria

  • Khaliqur RahmanEmail author
  • Dinesh Chandra


Paroxysmal nocturnal hemoglobinuria (PNH) is a nonmalignant, clonal hematopoietic stem cell disorder, which is characterized by features of hemolysis, thrombosis, and bone marrow (BM) failure. The genetic mechanism behind PNH is a somatic mutation in a gene known as phosphatidylinositol glycan class A (PIGA), present on chromosome “X.” PIGA gene is required for the synthesis of glycosylphosphatidylinositol (GPI), the anchor through which many proteins are attached to the cell membrane. These proteins are collectively known as GPI-anchored proteins (GPI-APs) [1, 2]. Among these GPI-APs are CD55 and CD59, the two important complement regulatory proteins. Absence of these leads to complement-mediated red blood cell lysis, one of the characteristic features of PNH [3, 4]. The term “PNH,” however, appears imprecise as only a fraction of patients presents with hemoglobinuria, which is also not always nocturnal.


Flow cytometry PNH FLAER High sensitivity assay 


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© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Department of HematologySanjay Gandhi Post Graduate Institute of Medical SciencesLucknowIndia

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