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Implementation of Optogenetics Technique for Neuron Photostimulation: A Physical Approach

  • Saurav Bharadwaj
  • Sushmita Mena
  • Dwarkadas Pralhaddas Kothari
Chapter

Abstract

Optogenetics is an opto-triggered neuron-switching technique in specific neuron clusters of the nervous system. Specifically, the chapter redefined the implantation of physical optode–electrode assembly on primate for simultaneous photo-switching and data recording from the complexly arranged neuron clusters. Specially, a number of modern commercial commutators are listed on the basis of flexible rotator optical joint, number of LEDs, magnetic base and low torque. However, the chapter highlights certain standard protocols used in the implantation of optode and electrode on the primate skull. For efficient optical stimulation and signal detection, it revised certain significant contributions on implementation of multimode fibres, photonic CMOS integrated, super flexible optofluidic ultrathin channel, iridium oxide electrodes and pulse width modulation control on different power levels and duty cycles. Specifically, the authors plotted different irradiance curves of mammalian brain tissues in linear scale at three wavelengths 473 nm, 561 nm and 630 nm. Successively, it is a collection of different wireless optogenetics systems of certain strength in each model as lightweight, super flexibility, optofluidic light delivery, small size, minimal heat emission and wide range output power control. Practically, a number of biomedical imaging techniques, that is, computer tomography, magnetic resonance imaging, micro-positron emission technology, micro-ultrasound, opto-micro-electrocorticography and electroretinography is implemented for studying the physiology and behaviour changes of primate under simultaneous photostimulation.

Keywords

Carousel commutators Tungsten electrodes Stereotaxic frame Irradiance Transcranial magnetic stimulation 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Saurav Bharadwaj
    • 1
  • Sushmita Mena
    • 2
  • Dwarkadas Pralhaddas Kothari
    • 3
  1. 1.Indian Institute of Information Technology GuwahatiGuwahatiIndia
  2. 2.Assam Down Town UniversityGuwahatiIndia
  3. 3.Indian Institute of Technology DelhiNew DelhiIndia

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