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Tissue Culture

  • P. M. Priyadarshan
Chapter

Abstract

Tissue culture is the in vitro aseptic (sterile) culture of cells, tissues and organs under controlled nutritional and environmental conditions. Two concepts, plasticity and totipotency (ability of a cell to give rise to new organism or part), are central to understanding plant tissue culture. It involves the use of small pieces of plant tissue (explants) which are cultured in a nutrient medium under sterile conditions. Using the appropriate growing conditions for each explant type, tissues can be induced to rapidly produce new shoots and roots. These plantlets can also be divided, usually at the shoot stage, to produce large numbers of new plantlets. The new plants can then be placed in soil and grown in the normal way.

Keywords

History Components of Tissue Culture Media Preparing the Plant Tissue Culture Medium Transfer of Plant Material to Tissue Culture Medium Micropropagation Protoplast Culture Somatic Embryogenesis and Synthetic Seeds Plant Tissue Culture Terminology 

Further Reading

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • P. M. Priyadarshan
    • 1
  1. 1.Erstwhile Deputy DirectorRubber Research Institute of IndiaKottayamIndia

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