Tissue Culture

  • P. M. Priyadarshan


Tissue culture is the in vitro aseptic (sterile) culture of cells, tissues and organs under controlled nutritional and environmental conditions. Two concepts, plasticity and totipotency (ability of a cell to give rise to new organism or part), are central to understanding plant tissue culture. It involves the use of small pieces of plant tissue (explants) which are cultured in a nutrient medium under sterile conditions. Using the appropriate growing conditions for each explant type, tissues can be induced to rapidly produce new shoots and roots. These plantlets can also be divided, usually at the shoot stage, to produce large numbers of new plantlets. The new plants can then be placed in soil and grown in the normal way.


History Components of Tissue Culture Media Preparing the Plant Tissue Culture Medium Transfer of Plant Material to Tissue Culture Medium Micropropagation Protoplast Culture Somatic Embryogenesis and Synthetic Seeds Plant Tissue Culture Terminology 

Further Reading

  1. Anis M, Ahmad N (2016) Plant tissue culture: propagation, conservation and crop improvement. Springer, SingaporeCrossRefGoogle Scholar
  2. Bhojwani SS, Grover A (1996) Tissue culture a novel source of genetic variations. Botanica 46:1–6Google Scholar
  3. Davey MR, Anthony P (2010) Plant cell culture: essential methods. Wiley-Blackwell, HobokenCrossRefGoogle Scholar
  4. Dodds J (2004) Experiments in plant tissue culture. Cambridge University Press, CambridgeGoogle Scholar
  5. George EF (1993) Plant propagation by tissue culture. In: Part 1, The Technology. Edington, Exegetics LtdGoogle Scholar
  6. Gray DJ, Purohit A, Triglano RN (1991) Somatic embryogenesis and development of synthetic seed technology. Crit Rev Plant Sci 10:33–61CrossRefGoogle Scholar
  7. Iliev et al. (2010) Plant micropropagation. In: Davey and Anthony P (eds.) Plant cell culture. WileyGoogle Scholar
  8. Kyte et al (2013) Plants from test tubes: an introduction to micropropagation. Timber press, PortlandGoogle Scholar
  9. Murashige T (1974) Plant propagation through tissue culture. Ann Rev Plant Physiol 25:135–166CrossRefGoogle Scholar
  10. Onishi N, Sakamoto Y, Hirosawa T (1994) Synthetic seeds as an application of mass-production of somatic embryos. Plant Cell Tissue Organ Cult 39:137–145CrossRefGoogle Scholar
  11. Redenbaugh K, Fujii JA, Slade D (1988) Encapsulated plant embryos. In: Mizrahi A (ed) Advances in biotechnological processes. Alan R. Liss Inc., New YorkGoogle Scholar
  12. Rihan HZ et al (2017) Artificial seeds (Principle, aspects and applications). Agronomy 7:71. Scholar
  13. Sathyanarayana BN (2007) Plant tissue culture: practices and new experimental protocols. New Delhi, I. K. InternationalGoogle Scholar
  14. Shahzad A et al (2017) Historical perspective and basic principles of plant tissue culture. In: Plant biotechnology: principles and applications. Springer, pp 1–36
  15. Smith R (2012) Plant tissue culture 3rd Edn. Techniques and experiments. Elsevier, AmsterdamGoogle Scholar
  16. Trigiano RN, Gray DJ (2010) Plant tissue culture, development, and biotechnology. Taylor and Francis. Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • P. M. Priyadarshan
    • 1
  1. 1.Erstwhile Deputy DirectorRubber Research Institute of IndiaKottayamIndia

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