Effect of Substrates on Azotobacter chroococcum-Enriched Vermicompost for Growth of Phaseolus

  • Supriya Sharma
  • Reshma Tuladhar
  • Yukti Basnet
  • Sarita Manandhar
  • Shanti Bhattarai
  • Anjana Singh
  • Ajit Varma
Part of the Microorganisms for Sustainability book series (MICRO, volume 13)


Inoculation of Azotobacter chroococcum in preparing organic compost by vermicomposting using Eisenia fetida (common names: red worm, brandling worm, panfish worm, trout worm, tiger worm, red wiggler worm, red Californian earthworm) can promote the growth of the Phaseolus bean. Various abiotic stresses, such as drought and salinity, are among the major environmental constraints that limit growth, productivity, and quality of plants. The growth promotion of Phaseolus bean with inoculation of A. chroococcum in the presence of vermicompost using different substrate combinations was assessed by a number of nodules, shoot length, root length, dry shoot weight, dry root weight, and nitrogen, phosphorus, and potassium (NPK) content of the plant. Among different substrates used, cow dung only and cow dung plus straw could be enriched with A. chroococcum with survival period up to 5 weeks. However, cow dung plus chopped grass and cow dung plus ground grass couldn’t be enriched with A. chroococcum. A significant positive response was noted in all growth parameters when the plant was inoculated with A. chroococcum in the presence of vermicompost compared to the untreated control plants. Cow dung plus straw had been used as a substrate for the enrichment of vermicomposting with A. chroococcum.


Vermicomposting Azotobacter Abiotic stress Nodules Untreated Enrichment 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Supriya Sharma
    • 1
  • Reshma Tuladhar
    • 1
  • Yukti Basnet
    • 1
  • Sarita Manandhar
    • 2
  • Shanti Bhattarai
    • 3
  • Anjana Singh
    • 1
  • Ajit Varma
    • 4
  1. 1.Central Department of MicrobiologyTribhuvan UniversityKathmanduNepal
  2. 2.Tri-Chandra Multiple CampusTribhuvan UniversityKathmanduNepal
  3. 3.Nepal Agricultural Research Council KhumaltarKathmanduNepal
  4. 4.Amity Institute of Microbial TechnologyAmity UniversityNoidaIndia

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