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In-vitro Techniques to Study Cell Signaling

  • Natasha Jaiswal
Chapter

Abstract

Cell is the smallest unit in both plants and animals which continuously communicate with each other or with their environment. Cells have evolved a variety of signaling mechanism involving a network of events which is required to transmit the signals. This whole process is an intricate meshwork of interactions involving the crossroads of chemistry, physics, and biology. Over the course of a hundred million years of evolution, scientists have invented a number of biochemical approaches to explore these millions of biological networks both in vitro (in single isolated cells) and in vivo (in an intact organism) in plants and animals. Some of the in vitro methods developed so far have been talked about in the chapter. Nevertheless, despite technical improvements in the global understanding of signal transduction, its internal structures and its highly integrated and extremely dynamic nature remains largely mysterious and efforts should be made to invent some more rapid and improved methods to better our understanding of signal transduction.

Keywords

Cell signaling In vitro approaches 

References

  1. Bari R, Jones JD. Role of plant hormones in plant defence responses. Plant Mol Biol. 2009;69:473.CrossRefGoogle Scholar
  2. Berrier AL, Mastrangelo AM, Downward J, Ginsberg M, LaFlamme S. Activated R-Ras, Rac1, PI 3-kinase and PKC [small element of] can each restore cell spreading inhibited by isolated integrin b1 cytoplasmic domains. J Cell Biol. 2000;151:1549–60.CrossRefGoogle Scholar
  3. Depuydt S, Hardtke CS. Hormone signalling crosstalk in plant growth regulation. Curr Biol. 2011;21:R365–73.CrossRefGoogle Scholar
  4. Fraire-Velázquez S, Rodríguez-Guerra R, Sánchez-Calderón L. Abiotic and biotic stress response crosstalk in plants. In: Shanker A, editor. Abiotic stress response in plants—physiological, biochemical and genetic perspectives. Rijeka: InTech; 2011. p. 3–26.Google Scholar
  5. Harvey L, Arnold B, S Lawrence Z, Paul M, David B, James D. Molecular Cell Biology. 4th ed. 2000.Google Scholar
  6. Hauser F, Waadt R, Schroeder JI. Evolution of abscisic acid synthesis and signaling mechanisms. Curr Biol. 2011;21:R346–55.CrossRefGoogle Scholar
  7. Jaspers P, Kangasjärvi J. Reactive oxygen species in abiotic stress signaling. Physiol Plant. 2010;138:405–13.CrossRefGoogle Scholar
  8. Leyser O. Auxin, self-organisation and the colonial nature of plants. Curr Biol. 2011;21:R331–7.CrossRefGoogle Scholar
  9. Lukowitz W, Gillmor CS, Scheible WR. Positional cloning in Arabidopsis. Why it feels good to have a genome initiative working for you. Plant Physiol. 2000;23:795–805.CrossRefGoogle Scholar
  10. Marques MC, Alonso-Cantabrana H, Forment H, et al. A new set of ESTs and cDNA clones from full-length and normalized libraries for gene discovery and functional characterization in citrus. BMC Genomics. 2009;10:428.CrossRefGoogle Scholar
  11. Matyus L. Fluorescence resonance energy transfer measurements on cell surfaces A spectroscopic tool for determining protein interactions. J Photochem Photobiol B. 1992;12:323–37.CrossRefGoogle Scholar
  12. Michaelson D, Silletti J, Murphy G, D’Eustachio P, Rush M, Philips MR. Differential localization of Rho GRPases in live cells: regulation by hypervariable regions and RhoGDI binding. J Cell Biol. 2001;152:111–26.CrossRefGoogle Scholar
  13. Ossowski L, Aguirre-Ghiso JA. Urokinase receptor and integrin partnership: coordination of signaling for cell adhesion, migration and growth. Curr Opin Cell Biol. 2000;12:613–20.CrossRefGoogle Scholar
  14. Peleman JD, van der Voort JR. Breeding by design. Trends Plant Sci. 2003;8:330–4.CrossRefGoogle Scholar
  15. Pérez-Alfocea F, Ghanem ME, Gómez-Cadenas A, Dodd I. Omics of root-to-shoot signaling under salt stress and water deficit. OMICs J Integr Biol. 2011;15:893–901.CrossRefGoogle Scholar
  16. Porter JC, Hogg N. Integrins take partners: cross-talk between integrins and other membrane receptors. Trends Cell Biol. 1998;8:390–6.CrossRefGoogle Scholar
  17. Santner A, Estelle M. Recent advances and emerging trends in plant hormone signalling. Nature. 2009;459:1071–8.CrossRefGoogle Scholar
  18. Sastry SK, Burridge K. Focal adhesions: a nexus for intracellular signaling and cytoskeletal dynamics. Exp Cell Res. 2000;261:25–36.CrossRefGoogle Scholar
  19. Schwartz MA, Baron V. Interactions between mitogenic stimuli, or a thousand and one connections. Curr Opin Cell Biol. 1999;11:197–202.CrossRefGoogle Scholar
  20. Schwartz MA, Shattil SJ. Signaling networks linking integrins and rho family GTPases. Trends Biochem Sci. 2000;25:388–91.CrossRefGoogle Scholar
  21. Sun TP. The molecular mechanism and evolution of the GA–GID1– DELLA signaling module in plants. Curr Biol. 2011;21:R338–45.CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Natasha Jaiswal
    • 1
  1. 1.Department of MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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