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Bioremediation Efficacy of Extracellular Chromate Reductase from Bacillus amyloliquefaciens (CSB 9) for Detoxification of Hexavalent Chromium

  • B. P. Rath
  • S. Das
  • H. Thatoi
Chapter

Abstract

Extracellular Cr(VI) reductase was produced by a chromate resistant bacterium Bacillus amyloliquefaciens with optimized physico-chemical parameters. Subsequently, the purified Cr(VI) reductase showed specific activity of 0.167 U/mg, 6% yield and 30.36-fold increase in purity. Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molecular weight (M) of the purified enzyme of interest was estimated to be ~116 KDa. The purified enzyme was further subjected for functional characterization (influence of pH, temperature and storage stability). The Cr(VI) reductase activity of the purified enzyme for temperature and pH optima was at 35 °C and 7.0 respectively on standard analysis conditions. Using potassium dichromate as substrate, the enzyme showed maximum activity (Vmax) of 3.5 U/mL with its corresponding KM value of 27.78 μM. The purified enzyme exhibited higher stability when treated with certain additives. These remarkable qualities found with this enzyme produced by B. amyloliquefaciens could make this an ideal candidate for bioremediation of Cr(VI) under a wide range of environmental conditions.

Keywords

Bacillus amyloliquefaciens Bioremediation Chromate reductase Electrophoretical homogeneity Enzyme stability 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • B. P. Rath
    • 1
  • S. Das
    • 1
  • H. Thatoi
    • 2
  1. 1.Department of Biotechnology, College of Engineering and TechnologyBiju Patnaik University of TechnologyBhubaneswarIndia
  2. 2.Department of BiotechnologyNorth Orissa UniversityBaripada, MayurbhanjIndia

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