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Analysis of Cell Morphology, Vitality, and Motility: A Study Related to Analysis of Human Sperm

  • Raghavendra MaggaviEmail author
  • Sanjay Pujari
  • C. N. Vijaykumar
Conference paper
Part of the Lecture Notes on Data Engineering and Communications Technologies book series (LNDECT, volume 28)

Abstract

Attributes such as the number of sperm with motility, morphology, and vitality is necessary for successful fertilization and term pregnancy in a semen sample. Many of the vitality procedures used to stain semen result in coloring of the live/dead sperm. But they are not only costly but in addition, we need fluorescent microscope which is investment of another 10 lakh extra, moreover system fails to distinguish their individual morphology, that play a key role in the fertilization process. In the first stage, we measured sperm morphology with normal/abnormal status using a Papanicolaou stain. In the second stage, evaluated sperm vitality using Eosin–Nigrosin dye which is cheaper compared to methods used for coloring of the live/dead sperm and also measures the morphology of the individual sperm with normal/abnormal status. In the third stage, measured sperm motility along with their paths with identification numbers automatically from a video taken by research microscope. All the above methods are implemented using IMAGEJ (Image Processing using Java) open-source software.

Keywords

CASA FFMPEG WHO IVF ICSI IMSI 

Notes

Acknowledgements

We are thankful to third author of this paper Dr.Vijaykumar C.N, Cheif Embryologist, Freelance Consultant, Belagavi, Karanataka, for the help to get human sperm samples. We have used leftover samples which have arrived to clinics/labs for analysis. Informed consent by patients to use the sample for reasearch purposes was obtained.

References

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    Organisation WH (1999) WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge University PressGoogle Scholar
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    Organization WH et al (2010) Who laboratory manual for the examination and processing of human semenGoogle Scholar
  3. 3.
    MacLeod J, Gold RZ (Sep 1951) The male factor in fertility and infertility: II. Spermatozoon counts in 1000 men of known fertility and in 1000 cases of infertile marriage. J Urol 66(3):436–449Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Raghavendra Maggavi
    • 1
    Email author
  • Sanjay Pujari
    • 2
  • C. N. Vijaykumar
    • 3
  1. 1.VTUBelagaviIndia
  2. 2.Angadi Institute of Technology and ManagementBelagaviIndia
  3. 3.BelagaviIndia

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