Isolated Tissues and Organs

  • Jayanta Kumar Patra
  • Swagat Kumar Das
  • Gitishree Das
  • Hrudayanath Thatoi
Part of the Learning Materials in Biosciences book series (LMB)


Over the years, the use of isolated tissue and/or organ preparations has provided biologists a convenient biological model to work without the systemic influences of the intact animal. The isolated organ/tissue preparations are generally carried out in groups of 2, 4, 8 or more and changes can easily be studied as compared to intact animals. Traditionally, the in vitro dose responses of various drugs are carried out in tissue organ baths by using tissue preparations from various species such as frog, mice, guinea pig, rabbit etc. Different tissue preparations including smooth or skeletal muscles, arterial rings or strips, uterine tissue or vas deferens, ileum, colon, atrial or ventricle, diaphragm etc. are used for these studies. Under controlled environment and perfused with an oxygenated physiological solution condition these experiments are performed.


  1. Badyal D (2008) Practical manual of pharmacology. Jaypee Brothers Medical Publishers, New DelhiCrossRefGoogle Scholar
  2. BM Swamy V, Jayaveera KN, Reddy V (2014) Experimental pharmacology and toxicology. S. Chand & Company, New DelhiGoogle Scholar
  3. Goyal RK (2017) Practical in pharmacology. B. S. Shah Prakashan, AhmedabadGoogle Scholar
  4. Kale SR, Kale RR (2017) Practical pharmacology and toxicology. Nirali Prakashan, MumbaiGoogle Scholar
  5. Katzung BG, Masters SB, Trevor AJ (2012) Basic & clinical pharmacology. McGraw-Hill, New YorkGoogle Scholar
  6. Medhi B (2017) Practical manual of experimental and clinical pharmacology. Jaypee Brothers Medical Publishers, New DelhiGoogle Scholar
  7. Ritter JM, Lewis LD, Mant TGK, Ferro A (2008) A text book of clinical pharmacology and therapeutics. Hodder Arnold, LondonCrossRefGoogle Scholar
  8. Salmon DM (2014) Practical pharmacology for the pharmaceutical sciences. Wiley, ChichesterGoogle Scholar
  9. Sierra LM, Gaivao I (eds) (2014) Genotoxicity and DNA repair: a practical approach. Springer protocols. Humana Press, LondonGoogle Scholar
  10. Singhal KC (1997) Pharmacology laboratory manual. CBS Publishers & Distributor, New DelhiGoogle Scholar
  11. Tripathi KD (2013) Essentials of medical pharmacology. Jaypee Brothers Medical Publishers, New DelhiGoogle Scholar
  12. Turner RA, Hebborn P (eds) (1971) Screening methods in pharmacology. Academic, New YorkGoogle Scholar
  13. Vogel HG (ed) (2002) Drug discovery and evaluation pharmacological assays. Springer, BerlinGoogle Scholar
  14. Woolley A (2008) A guide to practical toxicology. Informa Healthcare USA, New YorkCrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Jayanta Kumar Patra
    • 1
  • Swagat Kumar Das
    • 2
  • Gitishree Das
    • 3
  • Hrudayanath Thatoi
    • 4
  1. 1.Research Institute of Biotechnology & Medical Converged ScienceDongguk UniversityGoyang-siSouth Korea
  2. 2.Department of BiotechnologyCollege of Engineering and Technology, Biju Patnaik University of TechnologyOdishaIndia
  3. 3.Dongguk UniversityGoyang-siSouth Korea
  4. 4.Department of BiotechnologyNorth Orissa UniversityOdishaIndia

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