Comparative Analysis of Cytotoxic Potential of Crude Extracts and Fractionated Isolates from Moringa oleifera Lam.
According to World Health Organization, most of the world’s population depends upon plants as an important element in primary healthcare systems. Ayurveda is India’s oldest indigenous medicine system of plant drugs. It is known from for preventing or suppressing various tumors using natural drugs, one such being Moringa oleifera Lam. This plant has reported antioxidant properties for both fruits and leaves (Luqman S, Srivastava S, Kumar R, Maurya AK, Chanda D, Evid Based Complement Alternat Med 2012(December):e519084. https://doi.org/10.1155/2012/519084, 2011); moreover, its extracts have exhibited anticancer properties in vitro in case of hepatocarcinoma as well as antitumor-promoting activities for skin cancer in rat models (Guevara AP, Vargas C, Sakurai H, Fujiwara Y, Hashimoto K, Maoka T, Kozuka M, Ito Y, Tokuda H, Nishino H, Mutat Res/Genet Toxicol Environ Mutagen 440(2):181–188. https://doi.org/10.1016/S1383-5718(99)00025-X, 1999).
The aim of this study is to investigate the antiproliferative activity of various extracts from Moringa oleifera Lam. and isolate the active compounds. Fifteen extracts were prepared form dried leaves of Moringa oleifera Lam. using five solvent systems and three methods of extraction. In vitro screening was done using Schizosaccharomyces pombe and MCF-7 cell line. The fractionation of active crude extracts was performed by silica gel column chromatography and fractions evaluated for cytotoxicity. The aqueous, methanolic and hydromethanolic extracts exhibited cytotoxicity against MCF-7 cell line at lower concentrations compared to lymphocytes. Our findings showed the following: (a) the crude extracts of Moringa oleifera Lam. exhibited cytotoxic potential; (b) the extracts were selectively more toxic to tumor cell line compared to normal lymphocytes. (c) The crude extract showed better anti-proliferative activity compared to fraction separated. The reason could be that the compounds in crude extract have a synergistic effect resulting in better activity.
KeywordsAntiproliferative Schizosaccharomyces pombe MCF-7 Cytotoxic potential
- 1.Luqman S, Srivastava S, Kumar R, Maurya AK, Chanda D (2011) Experimental assessment of Moringa oleifera leaf and fruit for its antistress, antioxidant, and scavenging potential using in vitro and in vivo assays. Evid Based Complement Alternat Med 2012(December):e519084. https://doi.org/10.1155/2012/519084 CrossRefGoogle Scholar
- 3.Zhao T, Sun Q, Marques M, Witcher M, Zhao T, Sun Q, Marques M, Witcher M (2015) Anticancer properties of Phyllanthus emblica (Indian Gooseberry), anticancer properties of Phyllanthus emblica (Indian Gooseberry). Oxidative Med Cell Longev 2015(June):e950890. https://doi.org/10.1155/2015/950890 CrossRefGoogle Scholar
- 4.Wall ME, Wani MC, Cook CE, Palmer KH, McPhail AT, Sim GA (1966) Plant antitumor agents. I. The isolation and structure of camptothecin, a novel alkaloidal leukemia and tumor inhibitor from Camptotheca acuminata 1,2. J Am Chem Soc 88(16):3888–3890. https://doi.org/10.1021/ja00968a057 CrossRefGoogle Scholar
- 7.Kumar VV, Hussain Z, Verma M (2014) Antiinflammatory and antinociceptive activity of Moringa oleifera. J Biomed Pharm Res 3(1):14–21Google Scholar
- 9.Amaglo NK, Deng J, Bennett R, Rosa E, Domínguez-Perles R (2014) Phytochemical content of distinct Moringa oleifera L, tissues (Leaves, Stems, and Petioles) as affected by shoot biomass (Re-Growth) age. J Bioprocess Eng Biorefinery 3(3):232–241. https://doi.org/10.1166/jbeb.2014.1101 CrossRefGoogle Scholar
- 13.Raaman N (2006) Phytochemical techniques. New India Publishing, New IndiaGoogle Scholar
- 14.Harborne JB (2012) Phytochemical methods: a guide to modern techniques of plant analysis. Springer Science & Business Media, New YorkGoogle Scholar
- 16.Verma A, Laakso I, Seppänen-Laakso T, Huhtikangas A, Riekkola M-L (2007) A simplified procedure for indole alkaloid extraction from Catharanthus roseus combined with a semi-synthetic production process for vinblastine. Molecules 12(7):1307–1315. https://doi.org/10.3390/12071307 CrossRefPubMedPubMedCentralGoogle Scholar
- 20.Villarini M, Moretti M, Pasquini R, Scassellati-Sforzolini G, Fatigoni C, Marcarelli M, Monarca S, Rodrıguez AV (1998) In vitro genotoxic effects of the insecticide deltamethrin in human peripheral blood leukocytes: DNA damage (‘comet’ Assay) in relation to the induction of sister-chromatid exchanges and micronuclei. Toxicology 130(2–3):129–139. https://doi.org/10.1016/S0300-483X(98)00097-3 CrossRefPubMedGoogle Scholar
- 22.Khalafalla MM, Abdellatef E, Dafalla HM, Nassrallah AA, Aboul-Enein KM, Lightfoot DA, El-Deeb FE, El-Shemy HA (2010) Active principle from Moringaoleifera Lam leaves effective against two leukemias and a hepatocarcinoma. Afr J Biotechnol 9(49):8467–8471Google Scholar
- 25.Lee J-H, Yeon J-H, Kim H, Roh W, Chae J, Park H-O, Kim D-M (2012) The natural anticancer agent plumbagin induces potent cytotoxicity in MCF-7 human breast cancer cells by inhibiting a PI-5 kinase for ROS generation. PLoS One 7(9):e45023. https://doi.org/10.1371/journal.pone.0045023 CrossRefPubMedPubMedCentralGoogle Scholar