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A Glycomic Approach Towards Identification of Signature Molecules in CD34+ Haematopoietic Stem Cells from Umbilical Cord Blood

  • Suchandra Chowdhury
  • Kaushik Bhattacharya
  • Chandan Mandal
  • Susmita Mondal
  • Sayantani Sarkar
  • Sarmila Chandra
  • Subir Banerjee
  • Chitra MandalEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1112)

Abstract

Umbilical cord blood (UCB) is a powerful storehouse for normal CD34+ haematopoietic stem cells (HSCs), often used for allogeneic bone marrow (BM) transplantation in malignant and non-malignant diseases. The glycomic especially the sialoglycomic aspect of these HSCs has been unravelled in this study. Cell surface expression of the glycans with the related enzymatic activities has been compared with the BM of childhood acute lymphoblastic leukaemia, a common BM-associated malignancy. An enhanced cell surface expression of α2,3-linked sialic acid, P- and E-selectins, and intercellular adhesion molecule along with reduced expression of L-selectin distinguishes CD34+ HSCs of UCB from leukaemic samples. More importantly, high expression of O-acetylated sialoglycoproteins, a hallmark of lymphoblasts, is drastically reduced in the CD34+ HSCs of UCB and is substantiated by the low activity of sialylate-O-acetyltransferase and high sialidase activity. In contrast, a significant variation is evident in the expression of sialic acid, α2,6-linked sialic acids, and the sialyltransferase activity. Taken together, these studies indicate a few signature molecules, forming a unique glycomic template, which may be a potential indicator, reassuring the normal profile of these stem cells, to be used for future transplantation.

Keywords

CD34+ haematopoietic stem cell Glycomics Sialic acid Cell adhesion molecules Sialylate-O-acetyltransferase Sialidase 

Notes

Acknowledgement

This work received financial support from the CSIR, New Delhi, Govt. of India. CM sincerely acknowledges the financial support from JC Bose National Fellowship (DST) and Distinguished Biotechnology Research Professor (DBT).

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Suchandra Chowdhury
    • 1
  • Kaushik Bhattacharya
    • 1
  • Chandan Mandal
    • 1
  • Susmita Mondal
    • 1
  • Sayantani Sarkar
    • 1
  • Sarmila Chandra
    • 2
  • Subir Banerjee
    • 3
  • Chitra Mandal
    • 1
    Email author
  1. 1.Cancer Biology and Inflammatory Disorder DivisionCouncil of Scientific and Industrial Research (CSIR)-Indian Institute of Chemical BiologyKolkataIndia
  2. 2.Clinical Haematology ServiceKolkataIndia
  3. 3.Department of Anaesthesiology and critical careMedical College and HospitalKolkataIndia

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