Bioconversion of Waste Conversion Gases to Liquid Fuels: Challenges and Opportunities

  • Aastha Paliwal
  • H. N. ChanakyaEmail author
  • Himanshu Kumar Khuntia
Conference paper


Increasing emphasis towards biomethanation of food wastes, methane recovery from landfills are creating a large stock of methane which is inefficiently being converted to electricity in a low recovery mode. Conversion to methanol would instead substitute a high-value fossil fuel, increase capital recovery. Methanol is considered as one of key biofuel alternatives with high octane number, good storability and potential to be used in fuel cells. Commercially viable biological conversion of methane to methanol has still eluded technologists despite generation of methane from biomass via anaerobic digestion and thermochemical conversion to methanol. This paper examines challenges and opportunities for methane from biomethanation of MSW to be converted to methanol by the microbiological route with the help of methanotrophs. The microbiology and biochemistry of the process dictate that methane-oxidizing capability of methanotrophs need to be interrupted, chemically, biochemically or genetically and process optimized to enhance production of methanol, which is an intermediate in the methane oxidation process. The objective of this study was to amalgamate the knowledge of microbiology such as their isolation, metabolism and growth conditions along with the engineering challenges to mass produce methanol from methane. The current status and bottlenecks of this process are captured.


  1. 1.
    Dhussa A (2004) Biogas in India. MNRE, IndiaGoogle Scholar
  2. 2.
    Bamboriya ML (2013) Biogas generation, purification and bottling: development in India. IndiaGoogle Scholar
  3. 3.
    Chanakya HN, Malayil S (2012) Anaerobic digestion for bioenergy from agro-residues and other solid wastes—an overview of science, technology and sustainability. J Indian Inst Sci 92(1):111–144Google Scholar
  4. 4.
    Pedersen TH, Schultz RH (2012) Technical and economic assessment of methanol production from biogas. Department of Energy Technology, University of Aalborg, DenmarkGoogle Scholar
  5. 5.
    Yergin D, Bocca R (2012) Energy for economic growth. Report, Geneva, SwitzerlandGoogle Scholar
  6. 6.
    Shamsul NS et al (2014) An overview on the production of bio-methanol as potential renewable energy. Renew Sustain Energy Rev 33(42):578–588CrossRefGoogle Scholar
  7. 7.
    EIA (2017) International energy outlook 2017. Report, USGoogle Scholar
  8. 8.
    IEA (2015) India energy outlook. Report, Paris, FranceGoogle Scholar
  9. 9.
    Saraswat VK, Bansal R India’s Leapfrog to Methanol Economy. Retrieved from:
  10. 10.
    Biofuels, The Fuel of the Future 2010. Retrieved from
  11. 11.
    Anderson JE et al (2010) Octane numbers of ethanol- and methanol-gasoline blends estimated from molar concentrations. Energy Fuels 24(12):6576–6585CrossRefGoogle Scholar
  12. 12.
    Nicholas RJ (2003) The methanol story: a sustainable fuel for the future. J Sci Ind Res 62:97–105Google Scholar
  13. 13.
    Bio-Residue Map of India. Retrieved from:
  14. 14.
    Joshi R, Ahmed S (2016) Status and challenges of municipal solid waste management in India: a review. Cognet Environ Sci 2:1139434Google Scholar
  15. 15.
    Knittel K, Boetius A (2009) Anaerobic oxidation of methane: progress with an unknown process. Annu Rev Microbiol 63:311–334CrossRefGoogle Scholar
  16. 16.
    Anthony C (1982) The biochemistry of methylotrophs. Academic Press, London, p 431Google Scholar
  17. 17.
    Boetius A et al (2000) A marine microbial consortium apparently mediating anaerobic oxidation of methane. Nature 407:623–626CrossRefGoogle Scholar
  18. 18.
    Whittenbury R, Phillips KC, Wilkinson JF (1970) Enrichment, isolation, and some properties of methane-utilizing bacteria. Microbiology 61:205–218Google Scholar
  19. 19.
    Hanson RS, Hanson TE (1996) Methanotrophic bacteria. Microbiol Rev 60(2):439–471Google Scholar
  20. 20.
    Fei Q et al (2014) Bioconversion of natural gas to liquid fuel: opportunities and challenges. Biotechnol Adv 3:596–614CrossRefGoogle Scholar
  21. 21.
    Sharp CE et al (2014) Distribution and diversity of verrucomicrobia methanotrophs in geothermal and acidic environments. Environ Microbiol 16(6):1867–1878CrossRefGoogle Scholar
  22. 22.
    Hutton WE, Zobell CE (1949) The occurrence and characteristics of methane-oxidizing bacteria in marine sediment. J Bacteriol 58(4):463Google Scholar
  23. 23.
    Bowman J (2006) The methanotrophs—the families methylococcaceae and methylocystaceae. Prokaryotes 5:266–289CrossRefGoogle Scholar
  24. 24.
    Hinrichs Ku et al (1999) Methane-consuming archaebacteria in marine sediments. Nature 398(6730):802–805CrossRefGoogle Scholar
  25. 25.
    Hwang IY et al (2015) Batch conversion of methane to methanol using Methylosinus trichosporium OB3b as biocatalyst. Microbiol Biotechnol 25(3):375–380CrossRefGoogle Scholar
  26. 26.
    Blanksby SJ, Ellison GB (2003) Bond dissociation energies of organic molecules. Acc Chem Res 36(4):255–263CrossRefGoogle Scholar
  27. 27.
    Park D, Lee J (2013) Biological conversion of methane to methanol. Korean J Chem Eng 30(5):977–987CrossRefGoogle Scholar
  28. 28.
    Hur DH et al (2016) Highly efficient bioconversion of methane to methanol using a novel type 1 Methylomonas sp. DH-1 newly isolated from brewery waste sludge. Chem Technol Biotechnol 92(2):311–318CrossRefGoogle Scholar
  29. 29.
    Hwang IY et al (2014) Biocatalytic conversion of methane to methanol as a key step for development of methane-based bio-refineries. J Microb Biotechnol 24(12):1597–1605CrossRefGoogle Scholar
  30. 30.
    Duan C (2011) High-rate conversion of methane to methanol by Methylosinus trichosporium OB3b. Biores Technol 102(15):7349–7353CrossRefGoogle Scholar
  31. 31.
    Frank J et al (1989) On the mechanism of inhibition of methanol dehydrogenase by cyclopropane-derived inhibitors. FEBS J 184(1):187–195Google Scholar
  32. 32.
    Furuto et al (1999) Semicontinuous methaol biosynthesis by Methylosinus trichosporium OB3b. J Mol Catal 144:257–261CrossRefGoogle Scholar
  33. 33.
    Xin JY et al (2009) Production of methanol from methane by methanotrophic bacteria. Biocatal Biotransform 22(3):225–229CrossRefGoogle Scholar
  34. 34.
    Kim HG et al (2010) Optimization of lab scale methanol production by Methylosinus trichosporium OB3b 15(3):476–480Google Scholar
  35. 35.
    Kalyuzhnaya MG et al (2015) Metabolic engineering in methanotrophic bacteria. Metab Eng 29:142–152CrossRefGoogle Scholar
  36. 36.
    Duan Z, Mao S (2006) A thermodynamic model for calculating methane solubility, density and gas phase composition of methane-bearing aqueous fluids from 273 to 523 K and from 1 to 2000 bar. Geochim Cosmochim Acta 70(13):3369–3386CrossRefGoogle Scholar
  37. 37.
    Vendruscolo F et al (2012) Determination of oxygen solubility in liquid media. ISRN Chemical EngineeringGoogle Scholar
  38. 38.
    Guo H et al (2013) In situ Raman spectroscopic study of diffusion coefficients of methane in liquid water under high pressure and wide temperatures. Fluid Phase Equilib 360:274–278CrossRefGoogle Scholar
  39. 39.
    Han P, Bartels DM (1996) Temperature dependence of oxygen diffusion in H2O and D2O. J Phys Chem 100(13):5597–5602CrossRefGoogle Scholar
  40. 40.
    Sheets JP et al (2016) Biological conversion of bio-gas to methanol using methanotrophs isolated from solid-state anaerobic digestate. Biores Technol 201:50–57CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Aastha Paliwal
    • 1
  • H. N. Chanakya
    • 1
    Email author
  • Himanshu Kumar Khuntia
    • 1
  1. 1.Centre for Sustainable Technologies (Formerly ASTRA)Indian Institute of ScienceBengaluruIndia

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