Bioremediation of Lead Compound Using Acinetobacter sp. 158; Optimization of Media and Cell Growth Dynamics

  • Moumita BoseEmail author
  • S. Datta
  • Pinaki Bhattacharya
Conference paper


The present investigation deals with an in-depth study on optimization of lead removal capacity of lead-resistant bacterium Acinetobacter sp. 158 as a response variable with limiting carbon source, pH, and rotational speed as non-interacting-type independent variables. The statistical optimization technique response surface methodology has been used for this purpose. The optimum parameters of the independent variables have been identified through 3D plots. A set of deterministic model equations have been developed through rigorous modeling and simulation to predict the cell growth dynamics of the lead-resistant bacterium Acinetobacter sp. 158. A set of programmed experiments have been conducted using the optimized value of the independent variables obtained through RSM to validate the proposed model equations.



Concentration of substrate glucose (kg/m3)


Concentration of biomass (kg/m3)


Maximum specific cell growth rate (hour−1)


Saturation constant for lead (kg/m3)


Time of incubation (hours)

\(\varvec{Y}_{{{\raise0.7ex\hbox{$\varvec{B}$} \!\mathord{\left/ {\vphantom {\varvec{B} \varvec{G}}}\right.\kern-0pt} \!\lower0.7ex\hbox{$\varvec{G}$}}}}\)

Yield factor



One of the authors (M.B.) gratefully acknowledges the University Grants Commissions, Govt. of India for providing financial support for this work.


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© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Chemical Engineering DepartmentJadavpur UniversityKolkataIndia
  2. 2.Department of Chemical EngineeringHeritage Institute of TechnologyKolkataIndia

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