Mass Culturing of Phytophagous Insects for Host Plant Resistance Studies

  • K. Kumar
  • G. Dhakshinamoorthy


Studies on host plant relationships/resistance necessitate availability of insects in large numbers. Different orders of insects may require different mass culture procedures. Contamination by microorganisms and low yield of quality insects are often the impediments encountered while mass culturing. Procedures for six chewing and sucking key pests have been outlined in this chapter.


Mass rearing Insect pests Aseptic condition Host plant relationships 



The authors would like to thank authorities of Pandit Jawaharlal Nehru College of Agriculture, Tamil Nadu, and Sri Ramakrishna Institute, Tamil Nadu, for their encouragement and support.


  1. Arivudainambi, S., & Selvanarayanan, V. (2000). Enhancing the efficacy and persistency of Spodoptera litura (Fab.) nuclear polyhedrosis virus using UV irradiation protectants (pp 1175–1176). NISCAIR-CSIR, India.Google Scholar
  2. Armes, N. J., Jadhav, D. R., & King, A. B. S. (1992, November 24–28). Pyrethroid resistance in the pod borer, Helicoverpa armigera. In Proceeding Brighton crop protection conference — Pests and diseases, Bracknel.Google Scholar
  3. Kumar, P., & Ballal, C. R. (1990). An improved oviposition cage and egg collection technique for the mass multiplication of Heliothis armigera Hubner. Indian Journal of Plant Protection, 18(2), 255–259.Google Scholar
  4. Lingappa, S. (1978). Development of artificial diet for mass rearing Sesamia inferens walker (Lepidoptera: Noctuidae) and screening for resistance in finger millet germplasm (p. 140). Ph. D. thesis submitted to UAS GKVK, Bengaluru. India.Google Scholar
  5. Miles, P. W., & Harrewijn, P. (1991). Discharge by aphids of secretions into dietary sources. Entomologia Experimentalis et Applicata, 59, 123–134.CrossRefGoogle Scholar
  6. Muthukumaran, N., & Selvanarayanan, V. (2008). Resistance in hybrid derivatives of Lycopersicon accessions against leaf caterpillar, Spodoptera litura Fab. Insect Science, 15, 141–146.CrossRefGoogle Scholar

Further Reading

  1. Gonzalez-Cabrera, J., Garcia-Cancino, M. D., Moreno-Carrillo, G., Sanchez-Gonzalez, J. A., & Arredondo-Bernal, H. C. (2018). Fresh banana as an alternative host for mass rearing Drosophila suzukii. Bulletin of Insectology, 71(1), 65–70.Google Scholar
  2. Mihm, J. A. (1982). Techniques for efficient mass rearing and infestations in screening for host plant resistance to corn ear worm, Heliothis zea (pp. 255–261) In Proceedings of the international workshops on Heliothis management, 15–20 November 1981, ICRISAT Centre, Patancheru, India. Eds: Reed, W and Kumble, V., ICRISAT.Google Scholar
  3. Singh, P. (1977). Artificial diets for insects, mites, and spiders (p. 594). University of Minnesota, Nature.Google Scholar
  4. Singh, P. (1985). Handbook of insect rearing (Vol. 1, P. Singh, & R. F. Moore, Eds.) Elsevier, 1985 – Science.Google Scholar
  5. Schoonhoven, L. M., Van Loon, J. J. A. & Marcel, D. (2005). Insect-Plant bioecology. Second Edition. Oxoford University Press. pp: 421.Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • K. Kumar
    • 1
  • G. Dhakshinamoorthy
    • 2
  1. 1.Department of EntomologyPandit Jawaharlal Nehru College of AgricultureKaraikalIndia
  2. 2.Sri Ramakrishna InstituteCoimbatoreIndia

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