Physical and Chemical Mutagenesis Methods for Development of Insect-Resistant Crop Varieties

  • K. Saravanan
  • T. Sabesan


Selection of appropriate parents is the first step in breeding for pest resistance. Mutation breeding through physical and chemical mutagens can be resorted to, when the source of resistance is not available. This chapter gives a comprehensive look at the various options for physical and chemical mutagenesis along with their mechanism of action. Steps in mutation breeding for clonally and seed-propagated crops are explained which will enable researchers to take up resistance breeding through mutagenesis.


Mutation Physical agents Chemical agents Resistance breeding 



The authors would like to thank the authorities of the Annamalai University for their encouragement and support.


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Further Reading

  1. Balakrishna, D., Babu, K. S., Bhat, B. V., Vinodh, R., Sreedhar, M., Prasad, G. S., & Patil, J. V. (2015). Improved shoot fly resistant sources by gamma irradiation induced mutation in sorghum. Indian Journal of Plant Protection, 43(4), 403–410.Google Scholar
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  3. Bravo, A., Gill, S. S., & Soberon, M. (2007). Mode of action of Bacillus thuringiensis cry and Cyt toxins and their potential for insect control. Toxicon, 49(4), 423–435.CrossRefGoogle Scholar
  4. Lattanzio, V., Lattanzio, V. M., & Cardinali, A. (2006). Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. Phytochemistry: Advances in Research, 661, 23–67.Google Scholar
  5. Mandal, S., Kundu, P., Roy, B., & Mandal, R. K. (2002). Precursor of the inactive 2S seed storage protein from the Indian mustard Brassica juncea is a novel trypsin inhibitor characterization, post-translational processing studies, and transgenic expression to develop insect-resistant plants. Journal of Biological Chemistry, 277(40), 37161–37168.CrossRefGoogle Scholar
  6. Perlak, F. J., Fuchs, R. L., Dean, D. A., McPherson, S. L., & Fischhoff, D. A. (1991). Modification of the coding sequence enhances plant expression of insect control protein genes. Proceedings of the National Academy of Sciences, 88(8), 3324–3328.CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • K. Saravanan
    • 1
  • T. Sabesan
    • 1
  1. 1.Department of Genetics and Plant Breeding, Faculty of AgricultureAnnamalai UniversityChidambaramIndia

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