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Standard Techniques and Methods for Isolating, Selecting and Monitoring the Growth of Microalgal Strain

  • Md. Asraful AlamEmail author
  • Gul Muhammad
  • Abdul Rehman
  • Mohammad Russel
  • Mahfuzur Shah
  • Zhongming WangEmail author
Chapter

Abstract

The characterisation of microalgae is based on features, such as morphology, cell ultrastructure, pigments, photosynthetic products, reproduction, growth patterns, biomass and cellular proximate composition. These features are essential in identification, isolation, selection and cultivation of various microalgae for nutrition and as renewable resources, such as biofuels and biochemicals for human and animals. Although various methods have been used to isolate, select and monitor the growth of microalgal strain as described in the literature, few methods have limitations and not appropriately presented to users. Reviewing the standardised and validated methods for isolating and evaluating the characteristics of microalgae and providing a complete and simple report for the end users are necessary. This study aims to provide a complete and easily accessible guideline with all necessary standards and validated laboratory methods related to applied phycology, which can be used as reference by students and researchers who handle microalgae. In this chapter, major standard techniques for isolation and selection and calculation methods for monitoring microalgal growth are discussed with substantial number of flow charts and diagrams as the working manual in the field of applied phycology. The information provided in this chapter will be helpful for any users from the laboratory for the biomass production of commercial scale microalgae.

Notes

Acknowledgement

This research was supported by grants from the National Natural Science Foundation of China for Young International Scientists (21650110457) and National Key Research and Development Program-China (2016YFB0601004).

References

  1. Alam MA, Wang Z, Yuan Z. Generation and harvesting of microalgae biomass for biofuel production. In: Tripathi BN, Kumar D, editors. Prospects and challenges in algal biotechnology. Singapore: Springer; 2017. p. 89–111.CrossRefGoogle Scholar
  2. Allison S, Thomas R, Yves B. Lab on a chip technologies for algae detection: a review. J Biophotonics. 2012;5(8–9):661–72.Google Scholar
  3. Ben-Amotz A, Tornabene TG, Thomas WH. Chemical profile of selected species of microalgae with emphasis on lipids. J Phycol. 1985;21(1):72–81.CrossRefGoogle Scholar
  4. Benazzi G, Holmes D, Sun T, Mowlem MC, Morgan H.. Discrimination and analysis of phytoplankton using a microfluidic cytometer. In: IET nanobiotechnology, vol. 1. Institution of Engineering and Technology; 2007. p. 94–101.Google Scholar
  5. Black SK, Smolinski SL, Feehan C, Pienkos PT, Jarvis EE, Laurens LM. New method for discovery of starch phenotypes in growing microalgal colonies. Anal Biochem. 2013;432(2):71–3.CrossRefGoogle Scholar
  6. Brown MR, Dunstan GA, Jeffrey SW, Volkman JK, Barrett SM, Leroi J. The influence of irradiance on the biochemical composition of the Prymnesiophyte isochrysis sp. (clone t-iso)1. J Phycol. 1993;29(5):601–12.CrossRefGoogle Scholar
  7. Chu WL, Phang SM, Goh SH. Studies on the production of useful chemicals, especially fatty acids in the marine diatom Nitzschia conspicua Grunow. Hydrobiologia. 1994;285(1–3):33–40.CrossRefGoogle Scholar
  8. Collier JL. Flow cytometry and the single cell in phycology. J Phycol. 2000;36(4):628.CrossRefGoogle Scholar
  9. Cooksey KE, Guckert JB, Williams SA, Callis PR. Fluorometric determination of the neutral lipid content of microalgal cells using Nile Red. J Microbiol Methods. 1987;6(6):333–45.CrossRefGoogle Scholar
  10. Dean AP, Sigee DC, Estrada B, Pittman JK. Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae. Bioresour Technol. 2010;101(12):4499–507.CrossRefGoogle Scholar
  11. Deng Y-L, Chang J-S, Juang Y-J. Separation of microalgae with different lipid contents by dielectrophoresis. Bioresour Technol. 2013;135:137–41.CrossRefGoogle Scholar
  12. Doan TTY, Obbard JP. Improved Nile Red staining of Nannochloropsis sp. J Appl Phycol. 2011;23(5):895–901.CrossRefGoogle Scholar
  13. Griffiths MJ, Garcin C, Hille RPV, Harrison STL. Interference by pigment in the estimation of microalgal biomass concentration by optical density. J Microbiol Methods. 2011;85(2):119–23.CrossRefGoogle Scholar
  14. Hashemi N, Erickson JS, Golden JP, Jackson KM, Ligler FS. Microflow Cytometer for optical analysis of phytoplankton. Biosens Bioelectron. 2011;26(11):4263–9.CrossRefGoogle Scholar
  15. Hyka P, Lickova S, Přibyl P, Melzoch K, Kovar K. Flow cytometry for the development of biotechnological processes with microalgae. Biotechnol Adv. 2013;31(1):2.CrossRefGoogle Scholar
  16. Kim HS, Devarenne TP, Han A. Microfluidic systems for microalgal biotechnology: a review. Algal Res. 2018;30:149–61.CrossRefGoogle Scholar
  17. Laurens LM, Wolfrum EJ. High-throughput quantitative biochemical characterization of algal biomass by NIR spectroscopy; multiple linear regression and multivariate linear regression analysis. J Agric Food Chem. 2013;61(50):12307–14.CrossRefGoogle Scholar
  18. Marie D, Simon N, Vaulot D.. Phytoplankton cell counting by flow cytometry. 2005.Google Scholar
  19. Mayers JJ, Flynn KJ, Shields RJ. Rapid determination of bulk microalgal biochemical composition by Fourier-Transform Infrared spectroscopy. Bioresour Technol. 2013;148(11):215–21.CrossRefGoogle Scholar
  20. Mendoza Guzmán H, Jara Valido ADL, Carmona Duarte L, Freijanes Presmanes K. Estimate by means of flow cytometry of variation in composition of fatty acids from Tetraselmis suecica in response to culture conditions. Aquac Int. 2010;18(2):189–99.CrossRefGoogle Scholar
  21. Paper I, Renato M, Hunt S. Aquatic biomass: sustainable bio- energy from algae? 2009.Google Scholar
  22. Parvin M, Zannat MN, Habib MAB. Two important techniques for isolation of microalgae. Asian Fish Sci. 2007;20:117–24.Google Scholar
  23. Price CA, Mendiolamorgenthaler LR, Goldstein M, Breden EN, Guillard RR. Harvest of planktonic marine algae by centrifugation into gradients of silica in the CF-6 continuous-flow zonal rotor. Biol Bull. 1974;147(1):136.CrossRefGoogle Scholar
  24. Rodrigues LHR, Arenzon A, Raya-Rodriguez MT, Fontoura NF. Algal density assessed by spectrophotometry: a calibration curve for the unicellular algae Pseudokirchneriella subcapitata. J Environ Chem Ecotoxicol. 2011;3(8):225–8.Google Scholar
  25. Sheehan J. A look back at the US Department of energy’s aquatic species program: biodiesel from algae. Aquatic Plants Algae. 1998.Google Scholar
  26. Sieracki M, Poulton N, Crosbie N. Automated isolation techniques for microalgae. 2005.Google Scholar
  27. Sosik HM, Olson RJ, Armbrust EV. Flow cytometry in phytoplankton research. Dordrecht: Springer; 2010.CrossRefGoogle Scholar
  28. Steinberg MK, First MR, Lemieux EJ, Drake LA, Nelson BN, Kulis DM, Anderson DM, Welschmeyer NA, Herring PR. Comparison of techniques used to count single-celled viable phytoplankton. J Appl Phycol. 2012;24(4):751–8.CrossRefGoogle Scholar
  29. Wagner H, Liu Z, Langner U, Stehfest K, Wilhelm C. The use of FTIR spectroscopy to assess quantitative changes in the biochemical composition of microalgae. J Biophotonics. 2010;3(8–9):557–66.CrossRefGoogle Scholar
  30. Wikfors GH, Ferris GE, Smith BC. The relationship between gross biochemical composition of cultured algal foods and growth of the hard clam, Mercenaria mercenaria (L.). Aquaculture. 1992;108(1–2):135–54.CrossRefGoogle Scholar
  31. Xie B, Stessman D, Hart JH, Dong HL, Wang YJ, Wright DA, Nikolau BJ, Spalding MH, Halverson LJ. High-throughput fluorescence-activated cell sorting for lipid hyperaccumulating Chlamydomonas reinhardtii mutants. Plant Biotechnol J. 2015;12(7):872–82.CrossRefGoogle Scholar
  32. Xin M, Yang JM, Xin X, Lei Z, Nie QJ, Mo X. Biodiesel production from oleaginous microorganisms. Renew Energy. 2009;34(1):1–5.CrossRefGoogle Scholar
  33. Zhu CJ, Lee YK. Determination of biomass dry weight of marine microalgae. J Appl Phycol. 1997;9(2):189–94.CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.CAS Key Laboratory of Renewable EnergyGuangzhou Institute of Energy Conversion, Chinese Academy of SciencesGuangzhouChina
  2. 2.Tianjin Key Laboratory of Indoor Air Environmental Quality Control, School of Environmental Science and TechnologyTianjin UniversityTianjinChina
  3. 3.Laboratory of Soil and Groundwater Pollution Remediation Simulation, Environmental Science and Ecology Department, School of Environmental Science and EngineeringTianjin UniversityTianjinChina
  4. 4.School of Food and Environment, Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of EducationDalian University of TechnologyPanjinChina
  5. 5.Manatee Holdings Ltd.CourtenayCanada

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