Standard Techniques and Methods for Isolating, Selecting and Monitoring the Growth of Microalgal Strain

  • Md. Asraful AlamEmail author
  • Gul Muhammad
  • Abdul Rehman
  • Mohammad Russel
  • Mahfuzur Shah
  • Zhongming WangEmail author


The characterisation of microalgae is based on features, such as morphology, cell ultrastructure, pigments, photosynthetic products, reproduction, growth patterns, biomass and cellular proximate composition. These features are essential in identification, isolation, selection and cultivation of various microalgae for nutrition and as renewable resources, such as biofuels and biochemicals for human and animals. Although various methods have been used to isolate, select and monitor the growth of microalgal strain as described in the literature, few methods have limitations and not appropriately presented to users. Reviewing the standardised and validated methods for isolating and evaluating the characteristics of microalgae and providing a complete and simple report for the end users are necessary. This study aims to provide a complete and easily accessible guideline with all necessary standards and validated laboratory methods related to applied phycology, which can be used as reference by students and researchers who handle microalgae. In this chapter, major standard techniques for isolation and selection and calculation methods for monitoring microalgal growth are discussed with substantial number of flow charts and diagrams as the working manual in the field of applied phycology. The information provided in this chapter will be helpful for any users from the laboratory for the biomass production of commercial scale microalgae.



This research was supported by grants from the National Natural Science Foundation of China for Young International Scientists (21650110457) and National Key Research and Development Program-China (2016YFB0601004).


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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.CAS Key Laboratory of Renewable EnergyGuangzhou Institute of Energy Conversion, Chinese Academy of SciencesGuangzhouChina
  2. 2.Tianjin Key Laboratory of Indoor Air Environmental Quality Control, School of Environmental Science and TechnologyTianjin UniversityTianjinChina
  3. 3.Laboratory of Soil and Groundwater Pollution Remediation Simulation, Environmental Science and Ecology Department, School of Environmental Science and EngineeringTianjin UniversityTianjinChina
  4. 4.School of Food and Environment, Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of EducationDalian University of TechnologyPanjinChina
  5. 5.Manatee Holdings Ltd.CourtenayCanada

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