Procedure to Develop Binodal Curve and Phase Diagram for Aqueous Two-Phase System

  • Zatul Iffah Mohd Arshad
  • Azura AmidEmail author


Purification of protein is normally involved an expensive downstream processing step. For large scale manufacturing facilities, identification of an economical procedure is the most advantage. Aqueous Two-Phase System (ATPS) is a technique that suitable to be used in extracting protein from the mix culture supernatant. The process is also economical. However, there are a few tricky parts that the researchers need to master before this technique can be applied. Therefore, this chapter presents the step by step procedure in optimizing this technique.


Purification Recombinant protein Biodal curve Solvent-solvent extraction 


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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Department of Biotechnology Engineering, Faculty of EngineeringInternational Islamic University MalaysiaKuala LumpurMalaysia

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