Monitoring the Growth of Plant Cells in Suspension Culture
Cell suspension cultures provide a convenient way in understanding the dynamics growth of plant cells. This knowledge is crucial in order to manipulate all parameters related to their growth so that the potential of plants can be fully utilised. Growth can be defined as the increase of cell numbers, size or mass. In a growth cycle, cells will be experiencing four phases which are lag, logarithmic (exponential), stationary and death phase. The information and knowledge gained from growth kinetics parameters provide the basic platform for modelling and predicting the characteristics of cell culture. This chapter describes the procedures for fresh weight, dry weight, TCN, medium conductivity (pH) and residual sugar analysis.
KeywordsPlant cell suspension Cell growth Fresh weight Dry weight Total cell number Medium conductivity Residual sugar analysis Arabidopsis thaliana Ficus deltoidea
The authors would like to acknowledge the Ministry of Higher Education in Malaysia for funding this project under the Fundamental Research Grant Scheme (FRGS 14-145-0386).
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