Compacted sewage sludge (CSS), also known as reducing barrier, can be used as barrier for tailings of mines. CSS is an effective means to isolate and eliminate acid mine drainage (AMD) produced by tailings. We studied the dynamics of bacterial community structure diversity over 75 days in the CSS. Samples were taken from two seepage conditions: pH 2.1 sulfuric acid water (SA) and the synthetic AMD. Deionized water (DW) was used as control. We used PCR-DGGE technique, which is denaturing gradient gel electrophoresis. The results indicated that Clostridiales, Bacillaceae, and Carnobacteriaceae dominated in the CSS samples with different relative abundance ranged from 46.26% to 10.25% at the start point (SP) of seepage, the 41st day (T1) of seepage or at the 75th day (T2) of seepage, under different seepage conditions (DW, SA and AMD). By redundancy analysis on the influences between environmental factors and microbial-community, microbial- mechanisms differed.
Compacted sewage sludge Microbial community structure diversity PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) Acid mine drainage (AMD) Heavy metals potential mobility
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The research was supported by the Doctoral Program of Higher Education of China (No. 20110211110025) and the Natural Science Foundation of Gansu Provincial Science & Technology Planning Project, China (No.18JR3RA222).
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