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Identifying Active Methanotrophs and Mitigation of CH4 Emissions in Landfill Cover Soil

  • Raksha K. Rai
  • Jyoti K. Chetri
  • Stefan J. Green
  • Krishna R. Reddy
Conference paper
Part of the Environmental Science and Engineering book series (ESE)

Abstract

In the USA, municipal solid waste (MSW) landfills constitute one of the major anthropogenic sources of methane emissions. In the landfill cover soils employed at MSW landfills, aerobic methane-oxidizing bacteria (MOB) convert CH4 to CO2, thereby partially mitigating the CH4 emissions to the atmosphere. In this study, culture-dependent and culture-independent techniques were employed to evaluate methane oxidation capacity and to characterize the microbial community in landfill cover soil. Microcosms with synthetic landfill gas headspace were used to measure potential methane oxidation rates in landfill cover soil and in methanotrophs-enriched microbial consortia. The results demonstrate that the enriched landfill cover soil supported the growth of a diverse group of methanotrophic and methylotrophic microorganisms, and were dominated by Type I methanotrophs showing positive correlation with CH4 oxidation rates.

Keywords

Methanotrophs CH4 oxidation activity Landfill cover soil Microbial community 

Notes

Acknowledgements

This project is funded by the U.S. National Science Foundation (Grant CMMI #1724773), which is gratefully acknowledged.

References

  1. Bybee SM, Bracken-Grissom H, Haynes BD, Hermansen RA, Byers RL, Clement MJ et al (2011) Targeted amplicon sequencing (TAS): a scalable next-gen approach to multilocus, multitaxa phylogenetics. Genome Biol Evol 3:1312–1323CrossRefGoogle Scholar
  2. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK et al (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7(5):335CrossRefGoogle Scholar
  3. Cébron A, Bodrossy L, Chen Y, Singer AC, Thompson IP, Prosser JI, Murrell JC (2007) Identity of active methanotrophs in landfill cover soil as revealed by DNA-stable isotope probing. FEMS Microbiol Ecol 62(1):12–23CrossRefGoogle Scholar
  4. Edgar RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26(19):2460–2461CrossRefGoogle Scholar
  5. Green SJ, Venkatramanan R, Naqib A (2015) Deconstructing the polymerase chain reaction: understanding and correcting bias associated with primer degeneracies and primer-template mismatches. PLoS ONE 10(5):e0128122CrossRefGoogle Scholar
  6. Han JS, Mahanty B, Yoon SU, Kim CG (2016) Activity of a methanotrophic consortium isolated from landfill cover soil: response to temperature, pH, CO2, and porous adsorbent. Geomicrobiol J 33(10):878–885CrossRefGoogle Scholar
  7. Kallistova AY, Montonen L, Jurgens G, Münster U, Kevbrina MV, Nozhevnikova AN (2013) Culturable psychrotolerant methanotrophic bacteria in landfill cover soil. Microbiology 82(6):847–855CrossRefGoogle Scholar
  8. Moonsamy PV, Williams T, Bonella P, Holcomb CL, Höglund BN, Hillman G, Simen BB (2013) High throughput HLA genotyping using 454 sequencing and the fluidigm access array™ system for simplified amplicon library preparation. HLA 81(3):141–149Google Scholar
  9. Murrell JC, McDonald IR, Bourne DG (1998) Molecular methods for the study of methanotroph ecology. FEMS Microbiol Ecol 27(2):103–114CrossRefGoogle Scholar
  10. Semrau JD, DiSpirito AA, Yoon S (2010) Methanotrophs and copper. FEMS Microbiol Rev 34(4):496–531CrossRefGoogle Scholar
  11. Spokas KA, Bogner JE (2011) Limits and dynamics of methane oxidation in landfill cover soils. Waste Manag 31(5):823–832CrossRefGoogle Scholar
  12. United States Environmental protection Agency (USEPA 2018) Inventory of greenhouse gas emissions and sinks (1990–2016)Google Scholar
  13. Walters W, Hyde ER, Berg-Lyons D, Ackermann G, Humphrey G, Parada A, Apprill A (2016) Improved bacterial 16S rRNA gene (V4 and V4-5) and fungal internal transcribed spacer marker gene primers for microbial community surveys. Msystems 1(1):e00009–e00015CrossRefGoogle Scholar
  14. Wise MG, McArthur JV, Shimkets LJ (1999) Methanotroph diversity in landfill soil: isolation of novel type I and type II methanotrophs whose presence was suggested by culture-independent 16S ribosomal DNA analysis. Appl Environ Microbiol 65(11):4887–4897Google Scholar
  15. Whittenbury R, Phillips KC, Wilkinson JF (1970) Enrichment, isolation and some properties of methane-utilizing bacteria. Microbiology 61(2):205–218Google Scholar
  16. Yargicoglu EN, Reddy KR (2017) Microbial abundance and activity in biochar-amended landfill cover soils: evidence from large-scale column and field experiments. J Environ Eng 143(9):04017058CrossRefGoogle Scholar
  17. Zhang J, Kobert K, Flouri T, Stamatakis A (2013) PEAR: a fast and accurate illumina paired-end reAd mergeR. Bioinformatics 30(5):614–620CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Raksha K. Rai
    • 1
  • Jyoti K. Chetri
    • 1
  • Stefan J. Green
    • 2
  • Krishna R. Reddy
    • 1
  1. 1.Department of Civil and Materials EngineeringUniversity of Illinois at ChicagoChicagoUSA
  2. 2.Sequencing Core, Research Resources Center and Department of Biological SciencesUniversity of Illinois at ChicagoChicagoUSA

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