Identifying Active Methanotrophs and Mitigation of CH4 Emissions in Landfill Cover Soil
In the USA, municipal solid waste (MSW) landfills constitute one of the major anthropogenic sources of methane emissions. In the landfill cover soils employed at MSW landfills, aerobic methane-oxidizing bacteria (MOB) convert CH4 to CO2, thereby partially mitigating the CH4 emissions to the atmosphere. In this study, culture-dependent and culture-independent techniques were employed to evaluate methane oxidation capacity and to characterize the microbial community in landfill cover soil. Microcosms with synthetic landfill gas headspace were used to measure potential methane oxidation rates in landfill cover soil and in methanotrophs-enriched microbial consortia. The results demonstrate that the enriched landfill cover soil supported the growth of a diverse group of methanotrophic and methylotrophic microorganisms, and were dominated by Type I methanotrophs showing positive correlation with CH4 oxidation rates.
KeywordsMethanotrophs CH4 oxidation activity Landfill cover soil Microbial community
This project is funded by the U.S. National Science Foundation (Grant CMMI #1724773), which is gratefully acknowledged.
- Moonsamy PV, Williams T, Bonella P, Holcomb CL, Höglund BN, Hillman G, Simen BB (2013) High throughput HLA genotyping using 454 sequencing and the fluidigm access array™ system for simplified amplicon library preparation. HLA 81(3):141–149Google Scholar
- United States Environmental protection Agency (USEPA 2018) Inventory of greenhouse gas emissions and sinks (1990–2016)Google Scholar
- Wise MG, McArthur JV, Shimkets LJ (1999) Methanotroph diversity in landfill soil: isolation of novel type I and type II methanotrophs whose presence was suggested by culture-independent 16S ribosomal DNA analysis. Appl Environ Microbiol 65(11):4887–4897Google Scholar
- Whittenbury R, Phillips KC, Wilkinson JF (1970) Enrichment, isolation and some properties of methane-utilizing bacteria. Microbiology 61(2):205–218Google Scholar