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Toxicity Evaluation of Nano-Zero Valent Iron to Soil Indigenous Microorganisms

  • Iziquiel Cecchin
  • Eloisa Fernanda Tessaro
  • Cleomar Reginatto
  • Antonio Thome
  • Krishna R. Reddy
Conference paper
Part of the Environmental Science and Engineering book series (ESE)

Abstract

The objective of this study was to evaluate the toxicity of nZVI to the indigenous microorganisms of the soil. The toxicity of nZVI was assessed by the CO2 production for the soil native microorganisms. Different concentrations of nZVI were added to the soil (0, 4, 15 and 50 g/kg) for further analysis of the microbiological activity. For a better interpretation of toxicity effects, was used glucose (g/kg) as a carbon source. As soil mixtures, nZVI and glucose were inserted into sealed vials for an evaluation of the microbiological activity. The quantification of CO2 produced by the indigenous microbes respiration was performed by stoichiometry, using NaOH to absorb the gas and using HCl to determine the concentration. The analyzes were performed every 02 days, until a total monitoring time of 40 days. It was observed that at a concentration of 50 g/kg it has not released any carbon dioxide, demonstrating a maladaptation of the microorganisms under these conditions. The nZVI concentrations of 4 and 15 g/kg showed an increase of CO2 production, indicating an adaptation of the microorganisms as available conditions. Thus, the application of nZVI as remediation technology can be performed up to concentrations of 15 g/kg without causing damage to native soil microorganisms. However, applications above these may cause toxic and/or inhibitory effects to the microbes, restricting a possible combination of nano and bioremediation.

Keywords

Nanoremediation Toxicity Microorganism 

References

  1. NBR 14283 (1999) Residuos em solos - Determinajao da biodegradajao pelo metodo respiro-metrico, Rio de JaneiroGoogle Scholar
  2. NBR 6459 (1984) Solo - Determinajao do limite de liquidez, Rio de JaneiroGoogle Scholar
  3. NBR 6508 (1984) Graos de solos que passam na peneira 4.8 mm - determinajao de massa espedfica, Rio de JaneiroGoogle Scholar
  4. NBR 7180 (1984) Solo - Determinajao do limite de Plasticidade, Rio de JaneiroGoogle Scholar
  5. NBR 7181 (1984) Solo - Analise Granulometrica, Rio de JaneiroGoogle Scholar
  6. Associajao Brasileira de Normas Tecnicas. NBR 6457 (1986) Amostras de solo - Preparajao de ensaios de compactajao e caracterizajao, Rio de JaneiroGoogle Scholar
  7. ASTM International - ASTM D2487-11 (2011) standard practice for classification of soils for engineering purposes (Unified Soil Classification System). ASTM International, West Con- shohocken, PAGoogle Scholar
  8. Karn B, Kuiken T, Otto M (2011) Nanotechnology and in situ remediation: a review of the benefits and potential risks. Ciencia Saude Coletiva 16(1):165–178CrossRefGoogle Scholar
  9. Kim HJ, Phenrat T, Tilton RD, Lowry GV (2009) Fe0 nanoparticles remain mobile in porous media after aging due to slow desorption of polymeric surface modifiers. Environ Sci Technol 43:3824–3830CrossRefGoogle Scholar
  10. Kirschling T, Gregory K, Minkley N, Lowry G, Tilton R (2010) Impact of nanoscale zero valent iron on geochemistry and microbial populations. Environ Sci Technol 44:3474–3480CrossRefGoogle Scholar
  11. Mueller NC, Braun J, Bruns J, Cernik M, Rissing P, Rickerby D, Nowack B (2012) Application of nanoscale zero valent iron (nZVI) for groundwater remediationin Europe. Environ Sci Pollut 19:550–558CrossRefGoogle Scholar
  12. Ohlinger R, Schinner F, Kandeler E, Margesin R (1996) Methods in Soil Biology. Springer, BerlinGoogle Scholar
  13. Reginatto C (2017) Avaliajao Do Processo De Transporte E Remediajao Com Nano Ferro De Valencia Zero Em Um Solo Residual Contaminado Com Cromo Hexavalente. Thesis, University of Porto Alegre, Porto AlegreGoogle Scholar
  14. Spinelli LF, Schnaid F, Selbasc PA, Bento FM, Oliveira JR (2005) Enhancing bioreme-diation of diesel and gasoline in soil amended with an agroindustrial sludge. J Air Waste Manag Assoc 55:421–429CrossRefGoogle Scholar
  15. Streck EV, Kampf N, Dalmolin RSD et al (2008) Soils of Rio Grande do Sul. 2. EMATER/RS, Porto Alegre, BrazilGoogle Scholar
  16. Tedesco M, Anghinoni I, Volkweiss SJ, Mielniczuk J, Bohnen H, Gianello C, Stannl, JG, Biassani CA, Meurer E (1992) Apostila da Disciplina de Principios de Fertilidade de Solos, Universidade Federal do Rio Grande do SulGoogle Scholar
  17. Tosco T, Coisson M, Xue D, Sethi R (2012) Zerovalent iron nanoparticles for groundwater remediation: surface and magnetic properties, colloidal stability, and perspectives for field application. In: Chiolerio AAP (eds.) Nanoparticles Featuring Electromagnetic Properties: From Science to Engineering. Research Signpost, Kerala, pp 201–223Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Iziquiel Cecchin
    • 1
  • Eloisa Fernanda Tessaro
    • 2
  • Cleomar Reginatto
    • 2
  • Antonio Thome
    • 2
  • Krishna R. Reddy
    • 3
  1. 1.Federal University of Porto AlegreRio Grande do SulBrazil
  2. 2.University of Passo FundoRio Grande do SulBrazil
  3. 3.University of Illinois at ChicagoIllinoisUSA

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