Toxicity Evaluation of Nano-Zero Valent Iron to Soil Indigenous Microorganisms

  • Iziquiel CecchinEmail author
  • Eloisa Fernanda Tessaro
  • Cleomar Reginatto
  • Antonio Thome
  • Krishna R. Reddy
Conference paper
Part of the Environmental Science and Engineering book series (ESE)


The objective of this study was to evaluate the toxicity of nZVI to the indigenous microorganisms of the soil. The toxicity of nZVI was assessed by the CO2 production for the soil native microorganisms. Different concentrations of nZVI were added to the soil (0, 4, 15 and 50 g/kg) for further analysis of the microbiological activity. For a better interpretation of toxicity effects, was used glucose (g/kg) as a carbon source. As soil mixtures, nZVI and glucose were inserted into sealed vials for an evaluation of the microbiological activity. The quantification of CO2 produced by the indigenous microbes respiration was performed by stoichiometry, using NaOH to absorb the gas and using HCl to determine the concentration. The analyzes were performed every 02 days, until a total monitoring time of 40 days. It was observed that at a concentration of 50 g/kg it has not released any carbon dioxide, demonstrating a maladaptation of the microorganisms under these conditions. The nZVI concentrations of 4 and 15 g/kg showed an increase of CO2 production, indicating an adaptation of the microorganisms as available conditions. Thus, the application of nZVI as remediation technology can be performed up to concentrations of 15 g/kg without causing damage to native soil microorganisms. However, applications above these may cause toxic and/or inhibitory effects to the microbes, restricting a possible combination of nano and bioremediation.


Nanoremediation Toxicity Microorganism 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Iziquiel Cecchin
    • 1
    Email author
  • Eloisa Fernanda Tessaro
    • 2
  • Cleomar Reginatto
    • 2
  • Antonio Thome
    • 2
  • Krishna R. Reddy
    • 3
  1. 1.Federal University of Porto AlegreRio Grande do SulBrazil
  2. 2.University of Passo FundoRio Grande do SulBrazil
  3. 3.University of Illinois at ChicagoIllinoisUSA

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