Effects of N-Glycans on Glycoprotein Folding and Protein Dynamics

  • Yoko Amazaki
  • Hien Minh Nguyen
  • Ryo Okamoto
  • Yuta Maki
  • Yasuhiro KajiharaEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1104)


This chapter describes the folding of synthetic homogeneous glycosylpolypeptides into glycoproteins depending on the position and number of glycosylation sites and oligosaccharide structures. To evaluate the role of oligosaccharides in protein folding, we synthesized small glycoprotein models, homogeneous misfolded glycoproteins, and erythropoietins. In addition to these chemical syntheses, this chapter introduces a unique method for 15N-labeling of synthetic glycoproteins to enable structural analysis. Based on experimental results, it can be suggested that N-glycans stabilize the structure of glycoproteins.


Glycoprotein Glycoprotein folding Erythropoietin Crambin Ovomucoid Fractalkine 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Yoko Amazaki
    • 1
  • Hien Minh Nguyen
    • 2
  • Ryo Okamoto
    • 1
  • Yuta Maki
    • 1
  • Yasuhiro Kajihara
    • 1
    Email author
  1. 1.Department of ChemistryOsaka UniversityToyonakaJapan
  2. 2.School of Medicine, Vietnam National UniversityHo Chi Minh CityVietnam

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