ICTMI 2017 pp 41-51 | Cite as

Noninvasive Quantitative Tissue Biopsy Using Precise Optical Phantoms

  • V. Vijayaragavan
  • N. SujathaEmail author
Conference paper


Purpose Optical biopsy refers to the group of diagnostic optical techniques used to extract information regarding tissue composition in a completely noninvasive routine. Fabrication and characterization of precise optical phantoms are critical in the development of quantitative optical biopsy instrumentation. Biological tissue is a heterogeneous and multilayer structure, and currently available optical phantoms fail in replicating the desired tissue in layer dimensions and layer—specific composition. This paper demonstrates the entire optical biopsy procedure using precise multilayer phantoms and multivariate algorithms for a case study of scleroderma. Procedures Multilayer phantom mimicking human skin with dermal and epidermal layers, having desired thickness and optical properties was fabricated using spin coating methodology, calibrated to operate in the desired conditions. Unique phantom-making procedures were introduced to achieve layers of uniform thickness. The fabricated phantom is used as samples for diffuse reflectance and fluorescence spectroscopic studies to extract the information regarding the tissue composition. Results From the fluorescence and diffuse reflectance spectra of the normal and scleroderma skin phantoms, known values of chromophore and fluorophore concentration were estimated using the partial least square multivariate algorithm (PLSMA) and the results are analyzed. Conclusions The fabrication of precise multilayer optical phantoms as used in this article would a promising tool for extracting compositional information from specific tissues and hence would offer noninvasive means for the discrimination of healthy and malignant conditions of the target tissues.


Multilayer phantom Fluorescence Diffuse reflectance Quantitative spectroscopy Optical biopsy 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Biophotonics Laboratory, Department of Applied MechanicsIndian Institute of TechnologyChennaiIndia

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