Supercooling-Promoting (Anti-ice Nucleation) Substances

  • Seizo FujikawaEmail author
  • Chikako Kuwabara
  • Jun Kasuga
  • Keita Arakawa
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1081)


Studies on supercooling-promoting substances (SCPSs) are reviewed introducing name of chemicals, experimental conditions and the supercooling capability (SCC) in all, so far recognized, reported SCPSs and results of our original study are presented in order to totally show the functional properties of SCPSs which are known in the present state. Many kinds of substances have been identified as SCPSs that promote supercooling of aqueous solutions in a non-colligative manner by reducing the ice nucleation capability (INC) of ice nucleators (INs). The SCC as revealed by reduction of freezing temperature (°C) by SCPSs differs greatly depending on the INs. While no single SCPS that affects homogeneous ice nucleation to reduce ice nucleation point has been found, many SCPSs have been found to reduce freezing temperatures by heterogeneous ice nucleation with a large fluctuation of SCC depending on the kind of heterogeneous IN. Not only SCPSs increase the degree of SCC (°C), but also some SCPSs have additional SCC to stabilize a supercooling state for a long term to stabilize supercooling against strong mechanical disturbance and to reduce sublimation of ice crystals. The mechanisms underlying the diverse functions of SCPSs remain to be determined in future studies.


Supercooling-promoting substance Anti-ice nucleation substance Freezing of water Homogeneous ice nucleation Heterogeneous ice nucleation Emulsion freezing Droplet freezing Antifreeze proteins and glycoproteins Polyphenols 



Antifreeze glycoprotein protein


Antifreeze protein


Buffered MQ-water


Temperature at which 50% of the water sample is frozen


Ice nucleator


Ice nucleation bacteria


Ice nucleation capability


Ultrapure water


Supercooling capability


Supercooling-promoting substance



We appreciate the gift of polyphenol mixtures and crude tannin extracts by Amino Up Chemical Co. Ltd. (Japan), the gift of polyphenol mixtures by Taiyo Kagaku Co. Ltd. (Japan), and gift of AFP III from the Notched-fin eelpout by Dr. S. Tsuda, AIST (Japan). We also appreciate collaboration study with Cosmo Oil Lubricants Co. Ltd. (Japan), Asahi Kasei Chemicals Corporation (Japan), DENSO Corporation (Japan), Ishihara Sangyo Kaisha Ltd. (Japan), and Nisshin Seifun Group Inc. (Japan).


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Seizo Fujikawa
    • 1
    Email author
  • Chikako Kuwabara
    • 1
  • Jun Kasuga
    • 2
  • Keita Arakawa
    • 1
  1. 1.Research Faculty and Graduate School of AgricultureHokkaido UniversitySapporoJapan
  2. 2.Research Center for Global AgromedicineObihiro University of Agriculture and Veterinary MedicineObihiroJapan

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