Pretreatment Process and Its Synergistic Effects on Enzymatic Digestion of Lignocellulosic Material

  • Yu-Cai He
  • Cui-Luan Ma
  • Bin Yang


In this chapter, the progress of pretreatment for enhancing the enzymatic digestion of lignocellulosic material is introduced. Furthermore, the pretreatment process and its synergistic effects on enzymatic digestion of lignocellulosic material are discussed. In general, the lignocellulose structure is mainly composed by three major components (hemicellulose, cellulose, and lignin). Cellulose microfibrils are coated with amorphous hemicellulose matrices building holocellulose structures and severely protected by non-sugar lignin outside. To overcome the inherent structural recalcitrance and enhance the sequential enzymatic saccharification of lignocellulosic materials, pretreatment is an indispensable step to be developed for making cellulose more accessible to cellulases. Enzymatic hydrolysis that bioconverts the pretreated lignocellulosic material with cellulases into fermentable sugars is known as the most complex step in this biological process due to enzyme-related and substrate-related effects and substrate-enzyme interactions. Thus, topics are summarized including characteristics of cellulose (e.g., degree of polymerization, crystallinity, and accessible surface area) and other components (e.g., oligomeric xylan and lignin) released from the pretreatment of lignocellulosic material and their effects on the effectiveness of enzymatic saccharification.


Pretreatment Biomass Enzymatic saccharification Cellulose Hemicellulose Lignin 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Yu-Cai He
    • 1
    • 2
  • Cui-Luan Ma
    • 1
    • 2
  • Bin Yang
    • 3
  1. 1.Platform of Biofuels and Biobased Products, College of Pharmaceutical Engineering and Life ScienceChangzhou UniversityChangzhouPeople’s Republic of China
  2. 2.Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life SciencesHubei UniversityWuhanPeople’s Republic of China
  3. 3.Bioproducts, Sciences and Engineering Laboratory and Department of Biological Systems EngineeringWashington State UniversityRichlandUSA

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