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Effect of Chemical Elicitors on Pentacyclic Triterpenoid Production in In Vitro Cultures of Achyranthes aspera L.

  • L. Sailo
  • Vinayak Upadhya
  • Poornananda M. Naik
  • Neetin Desai
  • Sandeep R. Pai
  • Jameel M. Al-KhayriEmail author
Chapter

Abstract

Bioprocess technology for the production of phytochemicals from plant cell cultures mainly depends upon elicitation for enhancing the yields. The application has been successfully demonstrated in various plant species for a number of metabolites of interest. Achyranthes aspera L. is a highly traded medicinal plant known for a wide array of pharmacological properties. In this study, the effect of different concentrations of salicylic acid (SA), methyl jasmonate (MeJA), jasmonic acid (JA), and chitosan (CH) on growth and accumulation of betulinic acid (BA), oleanolic acid (OA), and ursolic acid (UA) in cultures of A. aspera was investigated using RP-UFLC technique. Results for in vitro cultures grown on various concentrations of selected elicitors (50, 100, and 200 μM) were collected and studied at 7-, 15-, and 30-day intervals. Two separate experiments for elicitors with and without plant growth regulators (PGRs), i.e., 6-benzylaminopurine 3.0 mg/L and thidiazuron 0.5 mg/L, were studied. Higher fresh and dry weights were observed in all the treated tubes as compared to control. Optimum cell growth along with higher BA content was observed in MeJA-treated cultures with and without PGRs. Increased OA content was evident in 30-day cultures growing on 100 and 200 μM MeJA supplemented with PGRs. Chitosan both in media supplemented with and without PGRs produced higher content of UA at 30th day. Furthermore, it becomes important to understand the biochemical conversions in light of the metabolic pathways so that we can use this data in maneuvering production of metabolites in A. aspera.

Keywords

Achyranthes aspera Elicitation Secondary metabolites Triterpenoids 

Notes

Acknowledgment

All the authors are thankful to their higher authorities for their help and support. SRP is indebted to SERB, DST, New Delhi financial assistance (No. SERB/LS-153/2013) for part of work provided during this work.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • L. Sailo
    • 1
  • Vinayak Upadhya
    • 2
  • Poornananda M. Naik
    • 3
  • Neetin Desai
    • 4
  • Sandeep R. Pai
    • 4
  • Jameel M. Al-Khayri
    • 3
    Email author
  1. 1.Regional Institute of Paramedical and Nursing Sciences (RIPANS)AizawlIndia
  2. 2.Department of Forest Products and UtilizationCollege of ForestrySirsiIndia
  3. 3.Department of Agricultural BiotechnologyCollege of Agriculture and Food Sciences, King Faisal UniversityAl-HassaSaudi Arabia
  4. 4.Amity Institute of Biotechnology (AIB), Amity UniversityMumbaiIndia

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