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Role of Molecular Marker in the Genetic Improvement of the Medicinal and Aromatic Plants

  • Anubha SharmaEmail author
  • Nitish Kumar
  • Iti Gontia Mishra
Chapter

Abstract

Several molecular markers have been developed for breeding major crops owing to their significance, ease, and suitability. Out of these DNA markers are frequently used ones; therefore, in this chapter, we describe the DNA markers to map major genes with regard to their principle, applicability, and methods. The two major classes of DNA markers are based on (i) DNA hybridization, e.g., restriction fragment polymorphism, DNA chips, etc.,. and (ii) polymerase chain reaction (PCR), e.g., SSR, RAPD, AFLP, and SNP. Developing trait-linked markers involves the segregation of populations demonstrating target traits followed by reliable phenotyping methods. With the help of these techniques, trait-linked markers may be used in two situations: (i) in the absence of any biological information and (ii) with available information about the trait.

Keywords

Aromatic plants Medicinal plants Isozyme Breeding Alleles Fingerprinting 

Abbreviations

AFLP

Amplified fragment length polymorphism

EST

Expressed sequence tag

GBS

Genotyping by sequencing

ISSR

Inter-simple sequence repeats

PCR

Polymerase chain reaction

QTL

Quantitative trait loci

RAPD

Random amplified polymorphic DNA

RFLP

Restriction fragment length polymorphism

SNP

Single nucleotide polymorphism

SSR

Simple sequence repeats

STR

Short tandem repeat

STSs

Sequence-tagged sites

Notes

Conflict of Interest

It is declared that the authors have no competing interests.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Anubha Sharma
    • 1
    Email author
  • Nitish Kumar
    • 2
  • Iti Gontia Mishra
    • 3
  1. 1.Amity Institute of Biotechnology, Amity UniversityNoidaIndia
  2. 2.Department of Biotechnology, School of Earth, Biological and Environmental SciencesCentral University of South BiharGayaIndia
  3. 3.Biotechnology CentreJawaharlal Nehru Agricultural UniversityJabalpurIndia

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