In Vitro Approaches for Conservation and Sustainable Utilization of Butea monosperma (Lam.) Taub. Var. Lutea (Witt.) Maheshwari: A Highly Valuable Medicinal Plant

  • Rajesh Yarra
  • Ramesh Mushke
  • Madhu Velmala


Medicinal plants are a globally valuable source of life-saving drugs. The biotechnology is the key tool to select, reproduce, and conserve the critical and endangered species of medicinal plants. Butea monosperma grasps a significant position in the pharmaceutical domain because of its enormous medicinal properties resulting from the presence of several classes of secondary metabolites. Poor seed viability, low seed germination rate, and genetic variability restrict propagation of B. monosperma. Overexploitation, severe habitat destruction, and restricted distributions are the major constraints of sustainable production of this important plant. The in vitro tissue culture and micropropagation of Butea monosperma – significant medicinal plant – have been well established. The quick and reproducible in vitro response to plant growth regulator treatments has emerged as an indispensable balance of genetic transformation studies for this plant species. In this chapter, in vitro regeneration approaches, advancements, and improvements in genetic transformation of B. monosperma are addressed. Finally, we propose conclusions and future prospects for this medicinally important tree species.


Butea monosperma Micropropagation Genetic transformation 


  1. Ambasta, B. P. (1994). The useful plants of India. New Delhi: CSIR.Google Scholar
  2. Anonymous (1988) Wealth of India – a dictionary of raw materials and industrial products, raw materials. Publications and information directorate, Vol 1. New Delhi, pp 341–346Google Scholar
  3. Bhargava, S. K. (1986). Estrogenic and postcoital anticonceptive activity in rats of butin isolated from Butea monosperma seed. Journal of Ethnopharmacology, 18(1), 95–101.CrossRefPubMedGoogle Scholar
  4. Bulle, M., Kota, S., Rathakatla, D., Aileni, M., Kokkirala, V. R., Gadidasu, K. K., & Abbagani, S. (2012). An efficient in vitro leaf-based regeneration and evaluation of genetic Fidelity using ISSR markers in Woodfordia fruticosa (L.) Kurz. Journal of Herbs Spices & Medicinal Plants, 18, 178–190.CrossRefGoogle Scholar
  5. Firdaus, R., & Mazumder, A. (2012). Review on Butea Monosperma. International Journal of Pharmaceutical Chemistry, 2, 1035–1039.Google Scholar
  6. Gunakkunru, A., Padmanaban, K., Thirumal, P., Pritila, J., Parimala, G., Vengatesan, N., Gnanasekar, N., Perianayagam, J. B., Sharma, S. K., & Pillai, K. K. (2005). Anti-diarrhoeal activity of Butea monosperma in experimental animals. Journal of Ethnopharmacology, 98, 241–244.CrossRefPubMedGoogle Scholar
  7. Jadhav, S. N., Ved, D. K., Ghate, U., Reddy, K. N., & Reddy, C. S. (2001). Proceedings of the workshop and conservation assessment and management planning for medicinal plants of Andhra Pradesh. Bangalore: FRLHT.Google Scholar
  8. Jha, A. K., Prakash, S., Jain, N., & Gupta, S. C. (2004). Micropropagation of Sesbania rostrata from the cotyledonary node. Biologia Plantarum, 48, 289–292.CrossRefGoogle Scholar
  9. Kasture, V. S., Chopde, C. T., & Deshmukh, V. K. (2000). Anticonvulsive activity of Albizzia lebbeck, Hibiscus rosasinesis and Butea monosperma in experimental animals. Journal of Ethnopharmacology, 71, 65–75.CrossRefPubMedGoogle Scholar
  10. Kirtikar, K. R., & Basu, B. D. (1935). Indian medicinal plants (2nd ed.). Allahabad.Google Scholar
  11. Mahender, A., Mahesh, D. M., & Murthy, E. N. (2014). In vitro seed germination and development of Butea monosperma (Lam.) Taub. Var. lutea (Willt.): A step for rehabilitation. International Journal of Multidisciplinary and Current Research, 2, 297–301.Google Scholar
  12. Margaret, W. K., Jane, W. K., Lucien, N. D., Eliud, M. N., Colombe, D., & Christophe, K. (2015). Micropropagation of an endangered medicinal and indigenous multipurpose tree species: Erythrina abyssinica. HortScience, 50, 738–743.Google Scholar
  13. Mehta, B. K., Dubey, A., Bokadia, M. M., & Mehta, S. C. (1983). Isolation and in vitro antimicrobial efficiency of Butea monosperma seed oil on human pathogenic bacteria and phytopathogenic fungi. Acta Microbiologica et Immunologica Hungarica, 30, 75–77.Google Scholar
  14. Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.CrossRefGoogle Scholar
  15. Prashanth, D., Asha, M. K., Amit, A., & Padmaja, R. (2001). Anthelmintic activity of Butea monosperma. Fitoterapia, 72, 421–422.CrossRefPubMedGoogle Scholar
  16. Rajesh, Y., Mallesham, B., Ramesh, M., & Murthy, E. N. (2016). In vitro conservation and genetic homogeneity assessment of Butea monosperma (Lam.) Taub. Var. lutea (Witt.) Maheshwari—A potential pharmaceutical legume tree. Journal of Applied Research on Medicinal and Aromatic Plants, 3(4), 195–199.CrossRefGoogle Scholar
  17. Ramesh, M., Pavan, U., Venugopal Rao, K., & Sadanandam, A. (2005). Micropropagation of Terminalia belliricaRoxb.—A sericulture and medicinal plant. In Vitro Cellular & Developmental Biology –Plant, 41, 320–323.CrossRefGoogle Scholar
  18. Rasool, R., Kamili, A. N., Ganai, B. A., & Akbar, S. (2009). Effect of BAP and NAA on shoot regeneration in Prunella vulgaris. Journal of Natural Sciences and Mathematics, 3, 21–26.Google Scholar
  19. Reddy CS, Reddy KN, Jadhav SN (2001) Threatened (medicinal) plants of Andhra Pradesh. Medicinal plants conservation Centre, EPTRI, HyderabadGoogle Scholar
  20. Rout, G. R., Senapati, S. K., & Aparajeta, S. (2008). Micropropagation of Acacia chundra (Roxb.) DC. HortScience, 35, 22–26.Google Scholar
  21. Samaranayake, K. M., Bandara, B. M., & Kumar, N. S. (1989). An antifungal constituent from the stem bark of Butea monosperma. Journal of Ethnopharmacology, 25, 73–75.CrossRefPubMedGoogle Scholar
  22. Tandon, R., Shivanna, K. R., & Ram, H. M. (2003). Reproductive biology of Butea monosperma (Fabaceae). Annals of Botany, 92, 715–723.CrossRefPubMedPubMedCentralGoogle Scholar
  23. Thirupathaiah A (2007) Isolation of new flavonoids and their antimicroabial activity form medicinal plants of Telangana region. Ph.D thesis, Kakatiya University, Warangal, IndiaGoogle Scholar
  24. Tippani, R., Yarra, R., Bulle, M., Porika, M., Abbagani, S., & Thammidala, C. (2013). In vitro plantlet regeneration and agrobacterium tumefaciens-mediated genetic transformation of Indian Kino tree (Pterocarpus marsupium Roxb.). Acta Physiologiae Plantarum, 35, 3437–3344.CrossRefGoogle Scholar
  25. Trigiano, R. N., Geneve, R. L., Merkle, S. A., & Preece, J. E. (1992). Tissue and cell cultures of woody legumes. Horticultural Reviews, 14, 265–332.Google Scholar
  26. Wagner, H., Geyer, B., Fiebig, M., Kiso, Y., & Hikino, H. (1986). Isoputrin and Butrin, the Antihepatotoxic principles of Butea monosperma flowers. Planta Medica, 52, 77–79.CrossRefGoogle Scholar
  27. Yadava, R. N., & Tiwari, L. (2005). A potential antiviral flavone glycoside from the seeds of Butea monosperma. Journal of Asian Natural Products Research, 7, 185–188.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Rajesh Yarra
    • 1
    • 2
  • Ramesh Mushke
    • 3
  • Madhu Velmala
    • 4
  1. 1.Department of Plant Sciences, School of Life SciencesUniversity of HyderabadHyderabadIndia
  2. 2.Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  3. 3.Aegis Agro Chemical India Pvt LtdHyderabadIndia
  4. 4.Department of BotanyGovernment Degree CollegeTiruvuruIndia

Personalised recommendations