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Diversity of Thermophiles in Terrestrial Hot Springs of Yunnan and Tibet, China

  • Wen-Dong Xian
  • Manik Prabhu Narsing Rao
  • En-Min Zhou
  • Lan Liu
  • Min Xiao
  • Wen-Jun LiEmail author
Chapter
Part of the Microorganisms for Sustainability book series (MICRO, volume 8)

Abstract

The Yunnan-Tibetan geothermal zone (YTGZ), located in western and southwestern China, harbors hundreds of hot springs with a wide range of temperatures and pH. These hot springs provide large and diverse niche for thermophilic microorganisms. In this chapter, we will discuss culture-dependent and culture-independent studies that have been conducted to understand Yunnan-Tibetan hot spring microbial diversity. Several novel taxa isolated and their uniqueness are listed. This chapter also sheds light on various physicochemical factors that structure the microbial diversity. The bioactive molecules and functional genes reported from these hot springs are also listed here.

Keywords

Terrestrial hot springs Thermophiles Yunnan Tibet Culture-dependent and culture-independent analysis Novel taxa 

Notes

Acknowledgment

This work was supported by the Key Project of International Cooperation of Ministry of Science and Technology (MOST) (No. 2013DFA31980), Science and Technology Infrastructure Work Project (No. 2015FY110100), Natural Science Foundation of China (Nos. 31470139 and 31600103), and China Postdoctoral Science Foundation No. 2017 M612796. W-J.L. was also supported by Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme (2014).

References

  1. Badhai J, Ghosh TS, Das SK (2015) Taxonomic and functional characteristics of microbial communities and their correlation with physicochemical properties of four geothermal springs in Odisha, India. Front Microbiol 6:1166.  https://doi.org/10.3389/fmicb.2015.01166 CrossRefPubMedPubMedCentralGoogle Scholar
  2. Bao Q, Tian Y, Li W, Xu Z, Xuan Z, Hu S, Dong W, Yang J, Chen Y, Xue Y, Xu Y, Lai X, Huang L, Dong X, Ma Y, Ling L, Tan H, Chen R, Wang J, Yu J, Yang H (2002) A complete sequence of the T. tengcongensis genome. Genome Res 12(5):689–700CrossRefPubMedPubMedCentralGoogle Scholar
  3. Berg IA, Kockelkorn D, Buckel W, Fuchs G (2007) A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea. Science 318:1782–1786CrossRefPubMedGoogle Scholar
  4. Bisht SS, Das NN, Tripathy NK (2011) Indian hot- water springs: a bird’s eye view. JoEECC 1(1):1–15Google Scholar
  5. Briggs BR, Brodie EL, Tom LM, Dong H, Jiang H, Huang Q, Wang S, Hou W, Wu G, Huang L, Hedlund BP, Zhang C, Dijkstra P, Hungate BA (2014) Seasonal patterns in microbial communities inhabiting the hot springs of Tengchong, Yunnan Province, China. Environ Microbiol 16(6):1579–1591CrossRefPubMedGoogle Scholar
  6. Brock TD (1997) The value of basic research: discovery of Thermus aquaticus and other extreme thermophiles. Genetics 146:1207–1210PubMedPubMedCentralGoogle Scholar
  7. Chan CS, Chan KG, Tay YL, Chua YH, Goh KM (2015) Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing. Front Microbiol 6:177.  https://doi.org/10.3389/fmicb.2015.00177 CrossRefPubMedPubMedCentralGoogle Scholar
  8. Chaudhuri B, Chowdhury T, Chattopadhyay B (2017) Comparative analysis of microbial diversity in two hot springs of Bakreshwar, West Bengal, India. Genom Data 12:122–129CrossRefPubMedPubMedCentralGoogle Scholar
  9. Chen C, Lin L, Peng Q, Ben K, Zhou Z (2002) Meiothermus rosaceus sp. nov. isolated from Tengchong hot spring in Yunnan, China. FEMS Microbiol Lett 216(2):263–268CrossRefPubMedGoogle Scholar
  10. Chen B, Ma C, Zhou S, Bu H, Jiang S, Li H, Nizhen (2009) Bacterial diversity of disused thermal vents in Tibetan Yangbajing Region. Wei Sheng Wu Xue Bao 49(2):217–226PubMedGoogle Scholar
  11. Chen JJ, Lin LB, Zhang LL, Zhang J, Tang SK, Wei YL, Li WJ (2012) Laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 62:38–42CrossRefPubMedGoogle Scholar
  12. Dong L, Ming H, Zhou EM, Yin YR, Liu L, Feng HG, Xian WD, Nie GX, Li WJ (2015) Crenobacter luteus gen. nov., sp. nov., isolated from a hot spring. Int J Syst Evol Microbiol 65:214–219CrossRefPubMedGoogle Scholar
  13. Duan YY, Ming H, Dong L, Yin YR, Zhang Y, Zhou EM, Liu L, Nie GX, Li WJ (2014) Streptomyces calidiresistens sp. nov., isolated from a hot spring sediment. Antonie Van Leeuwenhoek 106(2):189–196CrossRefPubMedGoogle Scholar
  14. Eloe-Fadrosh EA, Paez-Espino D, Jarett J, Dunfield PF, Hedlund BP, Dekas AE, Grasby SE, Brady AL, Dong H, Briggs BR, Li WJ, Goudeau D, Malmstrom R, Pati A, Pett-Ridge J, Rubin EM, Woyke T, Kyrpides NC, Ivanova NN (2016) Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs. Nat Commun 7:10476.  https://doi.org/10.1038/ncomms10476 CrossRefPubMedPubMedCentralGoogle Scholar
  15. Farmer J (1998) Thermophiles, early biosphere evolution, and the origin of life on Earth: implications for the exobiological exploration of Mars. J Geophys Res 103(E12):28457–28461CrossRefGoogle Scholar
  16. Gold T (1992) The deep, hot biosphere. Proc Natl Acad Sci U S A 89(13):6045–6049CrossRefPubMedPubMedCentralGoogle Scholar
  17. Gong N, Chen C, Xie L, Chen H, Lin X, Zhang R (2005) Characterization of a thermostable alkaline phosphatase from a novel species Thermus yunnanensis sp. nov. and investigation of its cobalt activation at high temperature. Biochim Biophys Acta 1750(2):103–111CrossRefPubMedGoogle Scholar
  18. Guo CL, Wang T, Zhu W, Zhang DH, Cui XL, Xu LH, Peng QA (2003) The phylotype of Thermus from the Rehai geothermal area, Tengchong, China. J Microbiol 41(2):152–156Google Scholar
  19. Habib N, Khan IU, Hussain F, Zhou EM, Xiao M, Ahmed I, Zhi XY, Li WJ (2017a) Caldovatus sediminis gen. nov., sp. nov., a moderately thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 67(11):4716–4721CrossRefPubMedGoogle Scholar
  20. Habib N, Khan IU, Hussain F, Zhou EM, Xiao M, Dong L, Zhi XY, Li WJ (2017b) Meiothermus luteus sp. nov., a slightly thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 67(8):2910–2914CrossRefPubMedGoogle Scholar
  21. He ZG, Zhong H, Li Y (2004) Acidianus tengchongensis sp. nov., a new species of acidothermophilic archaeon isolated from an acidothermal spring. Curr Microbiol 48(2):159–163CrossRefPubMedGoogle Scholar
  22. Hedlund BP, Cole JK, Williams AJ, Hou W, Zhou E, Li WJ, Dong H (2012) A review of the microbiology of the Rehai geothermal field in Tengchong, Yunnan Province, China. Geosci Front 3(3):273–288CrossRefGoogle Scholar
  23. Hedlund BP, Reysenbach A-L, Huang L, Ong JC, Liu Z, Dodsworth JA, Ahmed R, Williams AJ, Briggs BR, Liu Y, Hou W, Dong H (2015) Isolation of diverse members of the Aquificales from geothermal springs in Tengchong, China. Front Microbiol 6:157.  https://doi.org/10.3389/fmicb.2015.00157 CrossRefPubMedPubMedCentralGoogle Scholar
  24. Hochstein MP, Regenauer-Lieb K (1998) Heat generation associated with collision of two plates: the Himalayan geothermal belt. J Volcanol Geotherm Res 83(1–2):75–92CrossRefGoogle Scholar
  25. Hou W, Wang S, Dong H, Jiang H, Briggs BR, Peacock JP, Huang Q, Huang L, Wu G, Zhi X, Li W, Dodsworth JA, Hedlund BP, Zhang C, Hartnett HE, Dijkstra P, Hungate BA (2013) A comprehensive census of microbial diversity in hot springs of Tengchong, Yunnan Province China using 16S rRNA gene pyrosequencing. PLoS One 8:e53350.  https://doi.org/10.1371/journal.pone.0053350 CrossRefPubMedPubMedCentralGoogle Scholar
  26. Huang Q, Jiang H, Zhang C, Li W, Deng S, Yu B, Dong H (2010) Abundance of ammonia-oxidizing microorganisms in response to environmental variables of hot springs in Yunnan Province, China. Wei Sheng Wu Xue Bao 50(1):132–136PubMedGoogle Scholar
  27. Huang Q, Dong CZ, Dong RM, Jiang H, Wang S, Wang G, Fang B, Ding X, Niu L, Li X, Zhang C, Dong H (2011) Archaeal and bacterial diversity in hot springs on the Tibetan Plateau, China. Extremophiles 15(5):549–563CrossRefPubMedGoogle Scholar
  28. Jian H, Bo C, Xin D, Wei H, Xiuling J, Yunlin W, Lianbing L (2010) Diversity of Thermoacidophilic Sulfolobus in Hot Springs in Tengchong of Yunnan, China. Chin J Appl Environ Biol 16(5):692–696CrossRefGoogle Scholar
  29. Jiang C, Liu Y, Liu Y, Guo X, Liu SJ (2009) Isolation and characterization of ferrous- and sulfur-oxidizing bacteria from Tengchong solfataric region, China. J Environ Sci (China) 21(9):1247–1252CrossRefGoogle Scholar
  30. Jiang H, Huang Q, Dong H, Wang P, Wang F, Li W, Zhang C (2010) RNA-based investigation of ammonia-oxidizing archaea in hot springs of Yunnan Province, China. Appl Environ Microbiol 76(13):4538–4541CrossRefPubMedPubMedCentralGoogle Scholar
  31. Khan IU, Habib N, Hussain F, Xian WD, Amin A, Zhou EM, Ahmed I, Zhi XY, Li WJ (2017a) Thermus caldifontis sp. nov., a thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 67(8):2868–2872CrossRefPubMedGoogle Scholar
  32. Khan IU, Hussain F, Tian Y, Habib N, Xian WD, Jiang Z, Amin A, Yuan CG, Zhou EM, Zhi XY, Li WJ (2017b) Tibeticola sediminis gen. nov., sp. nov., a thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 67(5):1133–1139CrossRefPubMedGoogle Scholar
  33. Kikani BA, Sharma AK, Singh SP (2017) Metagenomic and culture-dependent analysis of the bacterial diversity of a hot spring reservoir as a function of the seasonal variation. Int J Environ Res 11:25.  https://doi.org/10.1007/s41742-017-0003-9 CrossRefGoogle Scholar
  34. Kumar B, Trivedi P, Mishra AK, Pandey A, Palni LM (2004) Microbial diversity of soil from two hot springs in Uttaranchal Himalaya. Microbiol Res 159:141–146CrossRefPubMedGoogle Scholar
  35. Lett MC, Muller D, Lievremont D, Silver S, Santini J (2012) Unified nomenclature for genes involved in prokaryotic aerobic arsenite oxidation. J Bacteriol 194(2):207–208CrossRefPubMedPubMedCentralGoogle Scholar
  36. Li H, Yang Q, Li J, Gao H, Li P, Zhou H (2015) The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China. Sci Rep 5:17056.  https://doi.org/10.1038/srep17056 CrossRefPubMedPubMedCentralGoogle Scholar
  37. Liao Z (2018) Thermal springs and geothermal energy in the Qinghai-Tibetan Plateau and the surroundings, Springer hydrogeology. Springer, Singapore, pp 5–10Google Scholar
  38. Lin LB, Chen CY, Peng Q, Ben KL, Zhou ZM (2002) Thermus rehai sp. nov., isolated from Rehai of Tengchong, Yunnan Province, China. J Basic Microbiol 42(5):337–344CrossRefPubMedGoogle Scholar
  39. Lin L, Hong W, Ji X, Han J, Huang L, Wei Y (2010) Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China. J Basic Microbiol 50(5):452–456CrossRefPubMedGoogle Scholar
  40. Lin L, Han J, Ji X, Hong W, Huang L, Wei Y (2011) Isolation and characterization of a new bacteriophage MMP17 from Meiothermus. Extremophiles 15(2):253–258CrossRefPubMedGoogle Scholar
  41. Liu YY, Chen ZW, Jiang CY, Liu SJ (2007) Isolation and characterization of Acidiphilium strain teng-A and its metabolism of fe (III) during pure- and mixed cultivation. Wei Sheng Wu Xue Bao 47(2):350–354PubMedGoogle Scholar
  42. Liu LJ, You XY, Guo X, Liu SJ, Jiang CY (2011a) Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon. Int J Syst Evol Microbiol 61:2395–2400CrossRefPubMedGoogle Scholar
  43. Liu LJ, You XY, Zheng H, Wang S, Jiang CY, Liu SJ (2011b) Complete genome sequence of Metallosphaera cuprina, a metal sulfide-oxidizing archaeon from a hot spring. J Bacteriol 193(13):3387–3388CrossRefPubMedPubMedCentralGoogle Scholar
  44. Liu L, Salam N, Jiao JY, Jiang HC, Zhou EM, Yin YR, Ming H, Li WJ (2016) Diversity of culturable thermophilic actinobacteria in hot springs in Tengchong, China and studies of their biosynthetic gene profiles. Microb Ecol 72(1):150–162CrossRefPubMedGoogle Scholar
  45. Lopez-Lopez O, Cerdan ME, Gonzalez-Siso MI (2013) Hot spring metagenomics. Life (Basel) 3(2):308–320Google Scholar
  46. Ming H, Yin YR, Li S, Nie GX, Yu TT, Zhou EM, Liu L, Dong L, Li WJ (2014) Thermus caliditerrae sp. nov., a novel thermophilic species isolated from a geothermal area. Int J Syst Evol Microbiol 64:650–656CrossRefPubMedGoogle Scholar
  47. Ming H, Duan YY, Guo QQ, Yin YR, Zhou EM, Liu L, Li S, Nie GX, Li WJ (2015) Meiothermus roseus sp. nov., a thermophilic bacterium isolated from a geothermal area. Antonie Van Leeuwenhoek 108(4):897–905CrossRefPubMedGoogle Scholar
  48. Ming H, Duan YY, Yin YR, Meng XL, Li S, Zhou EM, Huang JR, Nie GX, Li WJ (2016) Crenalkalicoccus roseus gen. nov., sp. nov., a thermophilic bacterium isolated from alkaline hot springs. Int J Syst Evol Microbiol 66(6):2319–2326CrossRefPubMedGoogle Scholar
  49. Nobre MF, Truper HG, ds Costa MS (1996) Transfer of Thermus ruber (Loginova et al. 1984), T. silvanus (Tenreiro et al. 1995), and T. chiliarophilus (Tenreiro et al. 1995) to Meiothermus gen. nov. as Meiothermus ruber comb. nov., Me. silvanus comb. nov., and Me. chilarophilus comb. nov., respectively, and emendation of the genus Thermus. Int J Syst Bacteriol 46:604–606CrossRefGoogle Scholar
  50. Oremland RS, Stolz JF (2003) The ecology of arsenic. Science 300(5621):939–944CrossRefPubMedGoogle Scholar
  51. Pathak AP, Rathod MG (2014) Cultivable bacterial diversity of terrestrial thermal spring of Unkeshwar, India. J Biochem Technol 5:814–818Google Scholar
  52. Peng TJ, Liu LJ, Liu C, Yang ZF, Liu SJ, Jiang CY (2015) Metallosphaera tengchongensis sp. nov., an acidothermophilic archaeon isolated from a hot spring. Int J Syst Evol Microbiol 65:537–542CrossRefPubMedGoogle Scholar
  53. Saiki T, Kimura R, Arima K (1972) Isolation and characterization of extremely thermophilic bacteria from hot springs. Agric Biol Chem 36(13):2357–2366CrossRefGoogle Scholar
  54. Satyanarayana T, Raghukumar C, Shivaji S (2005) Extremophilic microbes: diversity and perspectives. Curr Sci 89:78–90Google Scholar
  55. Sen R, Maiti NK (2014) Genomic and functional diversity of bacteria isolated from hot springs in Odisha, India. Geomicrobiol J 31:541–550CrossRefGoogle Scholar
  56. Shang SM, Qian L, Zhang X, Li KZ, Chagan I (2013) Themoanaerobacterium calidifontis sp. nov., a novel anaerobic, thermophilic, ethanol-producing bacterium from hot springs in China. Arch Microbiol 195(6):439–445CrossRefPubMedPubMedCentralGoogle Scholar
  57. Song ZQ, Zhi XY, Li WJ, Jiang H, Zhang CL, Dong H (2009) Actinobacterial diversity in hot springs in Tengchong (China), Kamchatka (Russia) and Nevada (USA). Geomicrobiol J 26:256–263CrossRefGoogle Scholar
  58. Song ZQ, Chen JQ, Jiang HC, Zhou EM, Tang SK, Zhi XY, Zhang LX, Zhang CLL, Li WJ (2010) Diversity of Crenarchaeota in terrestrial hot springs in Tengchong, China. Extremophiles 14(3):287–296CrossRefPubMedGoogle Scholar
  59. Song ZQ, Wang FP, Zhi XY, Chen JQ, Zhou EM, Liang F, Xiao X, Tang SK, Jiang HC, Zhang CL, Dong H, Li WJ (2013a) Bacterial and archaeal diversities in Yunnan and Tibetan hot springs, China. Environ Microbiol 15(4):1160–1175CrossRefPubMedGoogle Scholar
  60. Song ZQ, Wang L, Wang FP, Jiang HC, Chen JQ, Zhou EM, Liang F, Xiao X, Li WJ (2013b) Abundance and diversity of archaeal accA gene in hot springs in Yunnan Province, China. Extremophiles 17(5):871–879CrossRefPubMedGoogle Scholar
  61. Streit WR, Schmitz RA (2004) Metagenomics – the key to the uncultured microbes. Curr Opin Microbiol 7(5):492–498CrossRefPubMedGoogle Scholar
  62. Wang H, Zheng M, Huang X (2007) Cesium accumulation by bacterium Thermus sp. TibetanG7: hints for biomineralization of cesium-bearing geyserite in hot springs in Tibet, China. Chin Sci Bull 52:2680.  https://doi.org/10.1007/s11434-007-0353-3 CrossRefGoogle Scholar
  63. Wang S, Hou W, Dong H, Jiang H, Huang L, Wu G, Zhang C, Song Z, Zhang Y, Ren H, Zhang J, Zhang L (2013) Control of temperature on microbial community structure in hot springs of the Tibetan Plateau. PLoS One 8(5):e62901.  https://doi.org/10.1371/journal.pone.0062901 CrossRefPubMedPubMedCentralGoogle Scholar
  64. Wang S, Dong H, Hou W, Jiang H, Huang Q, Briggs BR, Huang L (2014) Greater temporal changes of sediment microbial community than its waterborne counterpart in Tengchong hot springs, Yunnan Province, China. Sci Rep 4:7479.  https://doi.org/10.1038/srep07479 CrossRefPubMedPubMedCentralGoogle Scholar
  65. Whitaker RJ, Grogan DW, Taylor JW (2003) Geographic barriers isolate endemic populations of hyperthermophilic archaea. Science 301(5635):976–978CrossRefPubMedGoogle Scholar
  66. Wu G, Jiang H, Dong H, Huang Q, Yang J, Webb L, Liu Q, Guo H, Yuan S, Li P (2015) Distribution of arsenite-oxidizing bacteria and its correlation with temperature in hot springs of the Tibetan-Yunnan geothermal zone in western China. Geomicrobiol J 32(6):482–493CrossRefGoogle Scholar
  67. Xian WD, Yin YR, Liu L, Yuan CG, Hussain F, Khan I, Habib N, Zhou EM, Li WJ (2016) Brevibacillus sediminis sp. nov., isolated from a hot spring. Int J Syst Evol Microbiol 66(2):548–553CrossRefPubMedGoogle Scholar
  68. Xiang XY, Dong XZ, Huang L (2003) Sulfolobus tengchongensis sp. nov., a novel thermoacidophilic archaeon isolated from a hot spring in Tengchong, China. Extremophiles 7(6):493–498CrossRefPubMedGoogle Scholar
  69. Xiang X, Chen L, Huang X, Luo Y, She Q, Huang L (2005) Sulfolobus tengchongensis spindle-shaped virus STSV1: virus-host interactions and genomic features. J Virol 79(14):8677–8686CrossRefPubMedPubMedCentralGoogle Scholar
  70. Xu LH, Tiang YQ, Zhang YF, Zhao LX, Jiang CL (1998) Streptomyces thermogriseus, a new species of the genus Streptomyces from soil, lake and hot-spring. Int J Syst Evol Microbiol 48(4):1089–1093Google Scholar
  71. Xue Y, Xu Y, Liu Y, Ma Y, Zhou P (2001) Thermoanaerobacter tengcongensis sp. nov., a novel anaerobic, saccharolytic, thermophilic bacterium isolated from a hot spring in Tengcong, China. Int J Syst Evol Microbiol 51(Pt 4):1335–1341CrossRefPubMedGoogle Scholar
  72. Xue Y, Zhang X, Zhou C, Zhao Y, Cowan DA, Heaphy S, Grant WD, Jones BE, Ventosa A, Ma Y (2015) Caldalkalibacillus thermarum gen. nov., sp. nov., a novel alkalithermophilic bacterium from a hot spring in China. Int J Syst Evol Microbiol 65:214–229CrossRefGoogle Scholar
  73. Yu TT, Ming H, Yao JC, Zhou EM, Park DJ, Hozzein WN, Kim CJ, Wadaan MA, Li WJ (2015) Thermus amyloliquefaciens sp. nov., isolated from a hot spring sediment sample. Int J Syst Evol Microbiol 65(8):2491–2495CrossRefPubMedGoogle Scholar
  74. Yuan CG, Chen X, Jiang Z, Chen W, Liu L, Xian WD, Asem MD, Zhi X-Y, Li WJ (2017) Altererythrobacter lauratis sp. nov, and Altererythrobacter palmitatis sp. nov., isolated from a Tibetan hot spring. Antonie Van Leeuwenhoek 110(8):1077–1086CrossRefPubMedGoogle Scholar
  75. Zhang CL, Ye Q, Huang Z, Li W, Chen J, Song Z, Zhao W, Bagwell C, Inskeep WP, Ross C, Gao L, Wiegel J, Romanek CS, Shock EL, Hedlund BP (2008) Global occurrence of archaeal amoA genes in terrestrial hot springs. Appl Environ Microbiol 74(20):6417–6426CrossRefPubMedPubMedCentralGoogle Scholar
  76. Zou P, Wb Z, Lei T, Wang JK (2006) Study on bioleaching of primary chalcopyrite ore with Thermoacidophilic Archae. Acta Metall Sin Engl 19(5):341–346CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Wen-Dong Xian
    • 1
  • Manik Prabhu Narsing Rao
    • 1
  • En-Min Zhou
    • 1
  • Lan Liu
    • 1
  • Min Xiao
    • 1
  • Wen-Jun Li
    • 1
    Email author
  1. 1.State Key Laboratory of Biocontrol, School of Life SciencesSun Yat-Sen UniversityGuangzhouChina

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