Impact of Seed Priming on the Modulation of Physico-chemical and Molecular Processes During Germination, Growth, and Development of Crops

  • Bandana Bose
  • Mahesh Kumar
  • Rajesh K. Singhal
  • Sananda Mondal


Seed is the prime input in agriculture sector, and production of quality seed is the immense challenge in front of agriculturist to achieve the goal of food security. Present sceario emphasis that the world population is increasing day by day resulting to quick exhaustion of natural resources leading to climate change which accelerates the issue of abiotic (heat, cold, drought, and salt) and biotic stress in plants. These abiotic and biotic stresses are often interrelated and cause undesirable physiological, morphological, biochemical, and molecular change that affect plant growth and development and ultimately yield. Time to time various plant breeding and molecular techniques developed to solve the problem of abiotic and biotic stresses. However, alternatively, some simple and economical techniques are also in race to address this problem. Seed priming is one of them, approved by many agriculturists for better crop stand establishment and growth, even under adverse environmental conditions. The present chapter deals with the different types of seed priming methods and their scope in mitigating abiotic and biotic stresses. Further, mechanisms of seed “priming-induced” physiological, biochemical, and molecular changes in regulation to stress tolerance were extensively explained in the light of the latest research work carried in this direction.


Seed priming Coating Seed hardening Abiotic stress Biotic stress 







Differentially expressed proteins




Late embryogenic abundance






Reactive oxygen species


Solid matrix priming


Superoxide dismutase


  1. Afzal I, Basra SMA, Farooq M, Nawaz A (2006) Alleviation of salinity stress in spring wheat by hormonal priming with ABA, salicylic acid and ascorbic acid. Int J Agric Biol 8:23. 1560-8530/2006/08-1-23-28 Google Scholar
  2. Afzal I, Butt A, Rehman HU, Basra SMA, Afzal A (2012) Alleviation of salt stress in fine aromatic rice by seed priming. Aust J Crop Sci 6:1401–1407Google Scholar
  3. Anaytullah, Bose B (2007) Nitrate-hardened seeds increase germination, amylase activity and proline content in wheat seedlings at low temperature. Physiol Mol Biol Plants 13:199–207Google Scholar
  4. Anaytullah, Srivastava AK, Bose B (2012) Impact of seed hardening treatment with nitrate salts on nitrogen and anti oxidant defense metabolisms in Triticum aestivum L under different sowing conditions. Vegetos 25:292–299Google Scholar
  5. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399PubMedCrossRefGoogle Scholar
  6. Araújo SDS, Paparella S, Dondi D, Bentivoglio A, Carbonera D, Balestrazzi A (2016) Physical methods for seed invigoration: advantages and challenges in seed technology. Front Plant Sci 7:646PubMedCentralCrossRefGoogle Scholar
  7. Asada K (1992) Ascorbate peroxidase–a hydrogen peroxide-scavenging enzyme in plants. Physiol Plant 85:235–241CrossRefGoogle Scholar
  8. Ashraf M (2009) Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnol Adv 27:84–93PubMedCrossRefGoogle Scholar
  9. Ashraf M, Foolad MR (2005) Pre-sowing seed treatment—a shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Adv Agron 88:223–271CrossRefGoogle Scholar
  10. Athar HUR, Khan A, Ashraf M (2008) Exogenously applied ascorbic acid alleviates salt-induced oxidative stress in wheat. Environ Exp Bot 63:224–231. CrossRefGoogle Scholar
  11. Basra SMA, Farooq M, Hafeez K, Ahmad N (2004) Osmohardening: a new technique for rice seed invigoration. Int Rice Res Notes 29:80–81Google Scholar
  12. Basra SMA, Afzal I, Anwar S, Shafique M, Haq A, Majeed K (2005) Effect of different seed invigoration techniques on wheat (Triticum aestivum L.) seeds sown under saline and non-saline conditions. J Seed Technol 28:36–45Google Scholar
  13. Basu RN, Chattopadhyay K, Pal P (1973) Maintenance of seed viability in rice (Oryza sativa L.) and Jute (Corchorus capsularis L. and C. olitorius L.). Indian Agric 18:76–79Google Scholar
  14. Bennett AJ, Whipps JM (2008) Dual application of beneficial microorganisms to seed during drum priming. Appl Soil Ecol 38:83–89CrossRefGoogle Scholar
  15. Bishaw Z, Niane AA, Gan Y (2007) Quality seed production. Lentil:349–383Google Scholar
  16. Bohnert HJ, Shen B (1999) Transformation and compatible solutes. Sci Hortic 78:237–260CrossRefGoogle Scholar
  17. Bose B, Mishra T (1992) Response of wheat seed to pre sowing seed treatment with Mg (NO3) 2. Ann Agric Res 13:132–136Google Scholar
  18. Bose B, Mishra T (1997) Effect of Mg salts on seed germination of Brassica sp. Cruciferae Newsletter (United Kingdom)Google Scholar
  19. Bose B, Mishra T (1999) Influence of pre-sowing soaking treatment in Brassica juncea seed with Mg salt on growth, nitrate reductase activity, total protein content and yield responses. Physiol Mol Biol Plant 5:83–88Google Scholar
  20. Bose B, Srivastava HS (1980) Proteolytic activity and nitrogen transfer in maize seed during imbibition. Biol Plant 22:414–419CrossRefGoogle Scholar
  21. Bose B, Srivastava HS (1982) Protein hydrolysis and nitrogen transfer during germination of maize seeds at various temperatures. Indian J Plant Physiol 25:141–148Google Scholar
  22. Bose B, Tandon A (1991) Effect of magnesium nitrate on metabolism in germinating maize seeds. Indian J Plant Physiol 34:69–71Google Scholar
  23. Bose B, Srivastava HS, Mathur SN (1982a) Effect of antibiotic on germination and protein activity of maize seeds. Indian J Plant Physiol 25:271–275Google Scholar
  24. Bose B, Srivastava RC, Mukherjee D, Mathur SN (1982b) Multipurpose use of NaHCO3 to Vigna mungo. In: Proceeding of the national symposium of biological nitrogen fixation, New Delhi, 298–309Google Scholar
  25. Bose B, Srivastava HS, Mathur SN (1982c) Effect of some nitrogenous salt on nitrogen transfer and protease activity in germination of maize (Zea Mays L.) seeds. Biol Plant 24:89–95CrossRefGoogle Scholar
  26. Bose B, Srivastava HS, Mathur SN (1983a) Partial purification and properties of protease from maize seed endosperm. Beitage Biologie der Pflanzen 58:383–391Google Scholar
  27. Bose B, Srivastava RC, Singh VK, Mathur SN (1983b) Sodium hydrogen carbonate: a potent electron donor for the nitrogen utilizing enzymes in Vigna mungo. Biochem Physiol Pflanz 178:443–448CrossRefGoogle Scholar
  28. Bose B, Kumar R, Kuril SK, Srivastava AK (2007) Hardening of mustard seeds with Mg(NO3)2 increases seed germination, vegetative growth, nitrogen assimilation and yield. Brassica 9:33–38Google Scholar
  29. Bose B, Kumari S, Anaytullah SAK, Kuril SK, Singh PK (2008) Phyto-toxic role of mercuric chloride on germination physiology and seedling growth of maize (Zea mays L.) var. Jaunpuri. Indian J Plant Physiol 13(3):284–290Google Scholar
  30. Bradford KJ (1986) Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Hort Sci (USA) 21:1105Google Scholar
  31. Callan NW, Mathre D, Miller JB (1990) Bio-priming seed treatment for biological control of Pythium ultimum preemergence damping-off in sh-2 sweet corn. Plant Dis 74:368–372CrossRefGoogle Scholar
  32. Chen K, Arora R (2011) Dynamics of the antioxidant system during seed osmopriming, post-priming germination, and seedling establishment in spinach (Spinacia oleracea). Plant Sci 180:212–220PubMedCrossRefGoogle Scholar
  33. Chen K, Arora R (2013) Priming memory invokes seed stress-tolerance. Environ Exp Bot 94:33–45CrossRefGoogle Scholar
  34. Chitra K, Ragupathi N, Dhanalakshmi K, Mareeshwari P, Indra N, Kamalakannan A, Sankaralingam A, Rabindran R (2008) Salicylic acid induced systemic resistant on peanut against Alternaria alternata. Arch Phytopathol Plant Protect 41:50–56CrossRefGoogle Scholar
  35. Dahal P, Bradford KJ, Jones RA (1990) Effects of priming and endosperm integrity on seed germination rates of tomato genotypes II. J Exp Bot 41:1441–1453CrossRefGoogle Scholar
  36. Devi TS, Shivaprakash MK, Maina CC (2013) Efficacy of seed bio-priming in enhancing seedling vigour of cucumber (Cucumis sativus L.) under biotic stress conditions. Mysore J Agric Sci 47:107–111Google Scholar
  37. Dieter F, Bouman F (1995) The seed: structure and function, Seed Development and Germination. Marcel Dekker, New York, pp 1–24Google Scholar
  38. Dollypan, Basu RN (1985) Mid-storage and pre-sowing seed treatments for lettuce and carrot. Sci Hortic 33:1026–1027Google Scholar
  39. Dong X, Bi H, Wu G, Ai X (2013) Drought-induced chilling tolerance in cucumber involves membrane stabilisation improved by antioxidant system. Int J Plant Prod 7:67–80Google Scholar
  40. Du LV, Tuong TP (2002) Enhancing the performance of dry-seeded rice: effects of seed priming, seedling rate, and time of seedling. In: Direct seeding: research strategies and opportunities. International Research Institute, Manila. 241–256Google Scholar
  41. Dursun A, Ekinci M (2010) Effects of different priming treatments and priming durations on germination percentage of parsley (Petroselinum crispum L.) seeds. Agric Sci 1:17–23Google Scholar
  42. El-Mohamedy RSR, Abd Alla MA, Badiaa RI (2006) Soil amendment and seed bio-priming treatments as alternative fungicides for controlling root rot diseases on cowpea plants in Nobaria Province. Res J Agric Biol Sci 2:391–398Google Scholar
  43. Farooq M, Basra SMA, Rehman H, Saleem BA (2008) Seed priming enhances the performance of late sown wheat (Triticumaestivum L.) by improving chilling tolerance. J Agron Crop Sci 194:55–60CrossRefGoogle Scholar
  44. Gallardo K, Job C, Groot SP, Puype M, Demol H, Vandekerckhove J, Job D (2001) Proteomic analysis of Arabidopsis seed germination and priming. Plant Physiol 126:835–848PubMedPubMedCentralCrossRefGoogle Scholar
  45. Gray D, Steckel JR (1983) Seed quality in carrots: the effects of seed crop plant density, harvest date and seed grading on seed and seedling variability. J Hortic Sci 58:393–401CrossRefGoogle Scholar
  46. Guan YJ, Hu J, Wang XJ, Shao CX (2009) Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. J Zhejiang University-Sci B 10:427–433CrossRefGoogle Scholar
  47. Halmer P (2006) Seed technology and seed enhancement. In: XXVII international Horticultural Congress-IHC2006: international symposium on seed enhancement and seedling production 771, pp 17–26Google Scholar
  48. Harris D, Joshi A, Khan PA, Gothkar P, Sodhi PS (1999) On-farm seed priming in semi-arid agriculture development and evaluation in maize, rice and chickpea in India using participatory methods. Exp Agric 35:15–29CrossRefGoogle Scholar
  49. Hu J, Zhu ZY, Song WJ, Wang JC, Hu WM (2005) Effect of sand priming on germination and field performance in direct-sown rice (Oryza sativa L.). Seed Sci Technol 33:243–248CrossRefGoogle Scholar
  50. Hussain M, Farooq M, Basra SM, Ahmad N (2006) Influence of seed priming techniques on the seedling establishment, yield and quality of hybrid sunflower. Int J Agric Biol 8:14–18Google Scholar
  51. Iqbal M, Ashraf M (2007) Seed treatment with auxins modulates growth and ion partitioning in salt-stressed wheat plants. J Integr Plant Biol 49:1003–1015CrossRefGoogle Scholar
  52. Jin S, Chen CCS, Plant AL (2000) Regulation by ABA of osmotic stressinduced changes in protein synthesis in tomato roots. Plant Cell Environ 23: 51–60CrossRefGoogle Scholar
  53. Jones ES, Liu CJ, Gale MD, Hash CT, Witcombe JR (1995) Mapping quantitative trait loci for downy mildew resistance in pearl millet. TAG Theor Appl Genet 91:448–456PubMedCrossRefGoogle Scholar
  54. Kausar A, Ashraf M (2003) Alleviation of salt stress in pearl millet (Pennisetum glaucum (L.) R. Br.) through seed treatments. Agronomie 23:227–234CrossRefGoogle Scholar
  55. Kazemi K, Eskandari H (2012) Does priming improve seed performance under salt and drought stress? J Basic Appl Sci Res 2:3503–3507Google Scholar
  56. Korkmaz A, Korkmaz Y (2009) Promotion by 5-aminolevulenic acid of pepper seed germination and seedling emergence under low-temperature stress. Sci Hortic 119:98–102CrossRefGoogle Scholar
  57. Krishnotar BB, Srivastava AK, Shahi JP (2009) Response of rabi maize crop to seed invigoration with magnesium nitrate and distilled water. Indian J Plant Physiol 14:71–77Google Scholar
  58. Kumar M, Pant B, Mondal S, Bose B (2016) Hydro and halo priming: influenced germination responses in wheat Var-HUW-468 under heavy metal stress. Acta Physiol Plant 38:217CrossRefGoogle Scholar
  59. Kuril SK (2010) Influence of seed hardening with Mg(NO3)2 and salicylic acid on timely and late sown mustard (Brassica juncea L. Czern and Coss) varieties, Ph.D. thesis, BHU, IndiaGoogle Scholar
  60. Lee S-S, Kim J-H (2000) Total sugars, $\alpha $-amylase activity, and germination after priming of normal and aged rice seeds. Kor J Crop Sci 45:108–111Google Scholar
  61. Lee SS, Kim JH, Hong SB, Yun SH, Park EH (1998) Priming effect of rice seeds on seedling establishment under adverse soil conditions. Korean J Crop Sci 43:194–198Google Scholar
  62. Liao XR, Sun Q (1994) Cobaltous ion inhibits the inducement of mungbean draught resistance by seed priming. Seed 3:29–32Google Scholar
  63. Lv Y, Zhang S, Wang J, Hu Y (2016) Quantitative proteomic analysis of wheat seeds during artificial ageing and priming using the isobaric tandem mass tag labeling. PLoS One 11:e0162851PubMedPubMedCentralCrossRefGoogle Scholar
  64. Malnassy PG (1971) Physiological and biochemical studies on a treatment hastening the germination of seeds at low temperature, Ph.D. thesis, Rutgers University, New BrunswickGoogle Scholar
  65. Manjunatha G, Raj SN, Shetty NP, Shetty HS (2008) Nitric oxide donor seed priming enhances defense responses and induces resistance against pearl millet downy mildew disease. Pestic Biochem Physiol 91:1–11CrossRefGoogle Scholar
  66. Mastouri F, Björkman T, Harman GE (2010) Seed treatment with Trichoderma harzianum alleviates biotic, abiotic, and physiological stresses in germinating seeds and seedlings. Phytopathology 100:1213–1221PubMedCrossRefGoogle Scholar
  67. Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R (2009) Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell Environ 33:453–467PubMedCrossRefGoogle Scholar
  68. Mittal R, Dubey RS (1995) Influence of sodium chloride salinity on polyphenol oxidase, indole 3-acetic acid oxidase and catalase activities in rice seedlings differing in salt tolerance. Trop Sci 35:141–149Google Scholar
  69. Moeinzadeh A, Sharif-Zadeh F, Ahmadzadeh M, Tajabadi F (2010) Biopriming of sunflower (‘Helianthus annuus’ L.) seed with ‘Pseudomonas fluorescens’ for improvement of seed invigoration and seedling growth. Aust J Crop Sci 4:564Google Scholar
  70. Mondal S, Vijai P, Bose B (2011) Role of seed hardening in rice variety Swarna (MTU 7029). Res J Seed Sci 4:157–165CrossRefGoogle Scholar
  71. Munir N, Aftab F (2009) The role of polyethylene glycol (PEG) pretreatment in improving sugarcane’s salt (NaCl) tolerance. Turk J Bot 33:407–415Google Scholar
  72. Musa AM, Harris D, Johansen C, Kumar JVDK (2001) Short duration chickpea to replace fallow after aman rice: the role of on-farm seed priming in the High Barind Tract of Bangladesh. Exp Agric 37:509–521CrossRefGoogle Scholar
  73. Nagaraju A, Sudisha J, Murthy SM, Ito SI (2012) Seed priming with Trichoderma harzianum isolates enhances plant growth and induces resistance against Plasmopara halstedii, an incitant of sunflower downy mildew disease. Australas Plant Pathol 41(6):609–620CrossRefGoogle Scholar
  74. Nayaka CS, Niranjana SR, Uday Shankar AC, Niranjan Raj S, Reddy MS, Prakash HS, Mortensen CN (2010) Seed biopriming with novel strain of Trichoderma harzianum for the control of toxigenic Fusarium verticillioides and fumonisins in maize. Arch Phytopathol Plant Protect 43:264–282CrossRefGoogle Scholar
  75. Nemec S, Datnoff LE, Strandberg J (1996) Efficacy of biocontrol agents in planting mixes to colonize plant roots and control root diseases of vegetables and citrus. Crop Prot 15:735–742CrossRefGoogle Scholar
  76. Pandey D, Bose B (2006) Influence of pre-sowing seed treatment with nitrate salts and different sowing dates on performance of mustard. Indian J Plant Physiol 11:261–265Google Scholar
  77. Pandita VK, Anand A, Nagarajan S, Seth R, Sinha SN (2010) Solid matrix priming improves seed emergence and crop performance in okra. Seed Sci Technol 38:665–674CrossRefGoogle Scholar
  78. Pant B, Bose B (2016) Mitigation of the influence of PEG-6000 imposed water stress on germination of halo primed rice seeds. Int J Agric Environ Biotechnol 9:275CrossRefGoogle Scholar
  79. Powell AA, Mathews S (1986) Cell membranes and seed leachate conductivity in relation to the quality of seeds for sowing. J Seed Technol 10:81–100Google Scholar
  80. Rajjou L, Duval M, Gallardo K, Catusse J, Bally J, Job C, Job D (2012) Seed germination and vigor. Annu Rev Plant Biol 63:507–533PubMedCrossRefGoogle Scholar
  81. Rashid A, Harris D, Hollington P, Ali S (2004a) On-farm seed priming reduces yield losses of mungbean (Vigna radiata) associated with mungbean yellow mosaic virus in the North West Frontier Province of Pakistan. Crop Prot 23:1119–1124CrossRefGoogle Scholar
  82. Rashid A, Harris D, Hollington P, Rafiq M (2004b) Improving the yield of mungbean Vigna radiata in the North West Frontiers province of Pakistan using on farm seed priming. Exp Agric 40:233–244CrossRefGoogle Scholar
  83. Rudrapal AB, Basu RN (1982) Use of chlorine and bromine in controlling mustard seed deterioration. Seed Res 9:188–191Google Scholar
  84. Salah SM, Yajing G, Dongdong C, Jie L, Aamir N, Qijuan H, … Jin H (2015) Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress. Sci Rep:5Google Scholar
  85. Sananda M, Bose B (2012) Kinetics studies on α-amylase extracted from germinating wheat endosperm of primed and non-primed seeds. Indian J Agric Biochem 25:137–141Google Scholar
  86. Sethy SK, Ghosh S (2013) Effect of heavy metals on germination of seeds. J Natl Sci Biol Med 4:272–275CrossRefGoogle Scholar
  87. Sharma MK, Bose B (2006) Effect of seed hardening with nitrate salts on seedling emergence, plant growth and nitrate assimilation of wheat (Triticumaestivum L.). Physiol Mol Biol Plants 12:173–176Google Scholar
  88. Sharma MK, Bose B, Shrivastava AK (2009) Effect of seed hardening with nitrate salts on physiological attributes at ear head emergence stage and yield of wheat (Triticum aestivum L.). Int J Agric Sci 5:439–442Google Scholar
  89. Sheidaie S, Divsalar M, Oskouei B, Sadeghi H, Rezvani E (2013) Seed hydro-priming application to alleviation of water stress during germination in sunflower hybrids (Helianthus annuus L.). Int J Agric Crop Sci 5:155Google Scholar
  90. Srivastava AK, Bose B (2012) Effect of nitrate seed priming on phenology, crop growth rate and yield attributes in rice (Oryza sativa L.). Vegetos 25:174–181Google Scholar
  91. Srivastava AK, Lokhande VH, Patade VY, Suprasanna P, Sjahril R, D’Souza SF (2010) Comparative evaluation of hydro-, chemo-, and hormonal-priming methods for imparting salt and PEG stress tolerance in Indian mustard (Brassica juncea L.). Acta Physiol Plant 32:1135–1144CrossRefGoogle Scholar
  92. Taylor AG, Klein DE, Whitlow T (1988) SMP: solid matrix priming of seeds. Sci Hortic 37:1–11CrossRefGoogle Scholar
  93. Taylor AG, Allen PS, Bennett MA, Bradford KJ, Burris JS, Misra MK (1998) Seed enhancements. Seed Sci Res 8:245–256. CrossRefGoogle Scholar
  94. Vander WC, Postaire O, Tournaire-Roux C, Boursiac Y, Maurel C (2006) Expression and inhibition of aquaporins in germinating Arabidopsis seeds. Plant Cell Physiol 47:1241–1250CrossRefGoogle Scholar
  95. Villiers TA, Edgcumbe DJ (1975) On the cause of seed determination in dry storage. Seed Sci Technol 3:761–774Google Scholar
  96. Wang X, Shen B (1991) Effect of MET-soaked seed on drought resistance of rice seedlings. Acta Phytophysiol Sin 17:105–108. (in Chinese with English abstract)Google Scholar
  97. Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures towards genetic engineering for stress tolerance. Planta 218:1–14PubMedPubMedCentralCrossRefGoogle Scholar
  98. Yildirim B, Yaser F, Ozpay T, Ozpay DT, Turkozu D, Terziodlu O, Tamkoc A (2008) Variations in response to salt stress among field pea genotypes (Pisum sativum sp. arvense L.). J Anim Vet Adv 7:907–910Google Scholar
  99. Zhou ZS, Guo K, Elbaz AA, Yang ZM (2009) Salicylic acid alleviate mercury toxicity by preventing oxidative stress in roots of Medicago sativa. Environ Exp Bot 65:27–34CrossRefGoogle Scholar

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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Bandana Bose
    • 1
  • Mahesh Kumar
    • 1
  • Rajesh K. Singhal
    • 1
  • Sananda Mondal
    • 2
  1. 1.Department of Plant Physiology, Institute of Agricultural SciencesBanaras Hindu UniversityVaranasiIndia
  2. 2.Plant Physiology Section, Department of ASEPANInstitute of Agriculture, Visva-BharatiSriniketanIndia

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