Role of SNP-Mediated Nitric Oxide Priming in Conferring Low Temperature Tolerance in Wheat Genotype (Triticum aestivum L.): A Case Study in Indian Northern Plains

  • Md. Afjal Ahmad
  • Pravin Prakash
  • H. B. Singh


Wheat is an economically important cereal crop grown in the vast stretch along the northern, western and part of Central Indian plains serving as livelihood tool for more than half of agrarian population of the country. The crop being grown in rabi (mid-November–mid-April) faces multifaceted abiotic stress threats among which low temperature stress being one of them. Nitric oxide has been well documented to counter many of these threats as also low temperature stress. In this context, a study was conducted to evaluate the effect of nitric oxide priming on certain morphophysiological and biochemical parameters in wheat genotype HD-2329 facing low temperature regime in the laboratory of the Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University. The several parameters under observations were per cent germination, germination rate, shoot length, root length, α-amylase activity and soluble sugar content both under normal (25 °C) and low temperature (5 °C) conditions. Seed priming with sodium nitroprusside (SNP, a nitric oxide donor) at 100 μM concentration resulted in enhanced performance of these parameters as compared to non-primed seeds both under normal and low temperature, but the effect was more pronounced at low temperature regime. Hence, it was concluded that NO priming of wheat seeds had a statistical significance in conferring low temperature tolerance in the crop, thus making it a fit priming model in a climate resilient era.


NO priming Low temperature stress Wheat 



The authors would like to acknowledge the cooperation obtained from the technical staff of the Department of Plant Physiology, I. Ag. Sc. BHU for smooth conduct of the research work.


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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Md. Afjal Ahmad
    • 1
  • Pravin Prakash
    • 1
  • H. B. Singh
    • 2
  1. 1.Department of Plant PhysiologyInstitute of Agricultural Sciences, BHUVaranasiIndia
  2. 2.Department of Mycology and Plant PathologyInstitute of Agricultural Sciences, BHUVaranasiIndia

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