Effect of Different Seed Priming Treatments on Germination and Seedling Establishment of Two Threatened Endangered Medicinal Plants of Darjeeling Himalaya

  • Dhiman Mukherjee


Present investigation was conducted under the aegis of Uttar Banga Krishi Viswavidyalaya, in Algarah (1900 m asl) under Darjeeling Himalaya. The aim was to study the effects of priming with different concentration of growth hormone, etc. on seed emergence characteristics and seedlings vigour of two endangered plant species of Swertia chirayita and Valeriana jatamansi. An experiment as randomized block design in 14 pretreatment seeds of S.chirayita and 15 pretreatment seeds of V. jatamansi with 3 replications was carried out in the field condition during November 2011 to November 2014. The pretreatments include osmo-priming with GA3 50, 100, 200, 400 and 800 ppm; IAA 50, 100, 200, 400, 800 ppm and KNO3 1, 2, 3 and 4% and hydro-priming (control with distilled water) for S.chirayita. In another experiment V. jatamansi seed osmo-priming with IBA 50, 100, 150, 200, 250 ppm; GA3 50,100, 150, 200, 250 ppm, kinetin 50, 100, 150, 200, 250 ppm and hydro-priming (control with distilled water). The maximum seed germination was observed with GA3 400 ppm and was at par with the GA3 200 ppm. Onset of germination was earliest, i.e. 19.00 days after sowing as registered by GA3 800 ppm for 12 h, which was statistically at par with IAA 200 ppm (20.33 days) and IAA 50 ppm (22 days). Mean germination time (MGT) 44.11 days in untreated (control) seeds which were significantly reduced to 27.34 days in seeds pretreated with IAA 400 ppm, which was 38.01% lower than the control and was at par with 29.63 and 30.67 days in seed pretreated with GA3 400 ppm and KNO3 (2%). Seeds treatment with GA3 400 ppm for 24 h showed maximum SV-I (71.11), which was 207.71% higher than the control. The highest SV-II of 54.18 was registered by KNO3 (2%), which was 68.16% higher than the control and was statistically at par with GA3 200 ppm. Germination energy of 1.16 was found in seeds pretreated with GA3 400 ppm, which was statistically at par with GA3 200 ppm, GA3 800 ppm, KNO3 (1%) and KNO3 (2%). Highest germination percentage seed of V. jatamansi was observed with the kinetin 200 ppm treated seeds and significantly better to all other treatments measures. Days require for onset of germination was earlier recorded with the IBA 200 and kinetin 250 ppm and was at par with the kinetin 200 and IBA 150 ppm. MGT was 33.03 days in untreated (control) seeds which were significantly reduced to 22.95 days with GA3 250 ppm, which was 31.88% lower than the control and was at par with kinetin 200, kinetin 250 and IBA 200 ppm primed seeds. Kinetin 100 ppm showed maximum SV-I (303.33), which was 484.15% higher than the control and was significantly better to all other 14 seed priming treatments. The highest SV-II of 190.06 was registered with kinetin 200 ppm, which was 362.32% higher than the control and was statistically better to other treatments. Maximum emergence index and germination energy were exhibited by kinetin 200 ppm.


Conservation Endangered plant Gibberellic acid IAA Himalaya Swertia chirayita Valeriana jatamansi 



Author is thankful to the Directorate of Medicinal and Aromatic Plants (ICAR), Anand, Gujarat for providing financial and technical support for this programme. The authors wish to acknowledge the critical help and support received from different forest range officers and medicinal plant growers of Darjeeling and Sikkim Himalaya for their full support throughout this programme.


  1. Abdul B, Andreson A (1973) Biochemical aspects of seed vigour. Hortic Sci 15:765–771Google Scholar
  2. Anon (1997) Biodiversity conservation prioritization project, conservation assessment and management plan (CAMP) for endemic medicinal plants in India. CIMP, Lucknow. 27Google Scholar
  3. Arteca RN (1996) Plant growth substances principles and applications. Chapter 3: chemistry, biological effects and mechanism of action. Chapman and Hall, New York, p 66Google Scholar
  4. Ashraf M, Foolad MR (2005) Pre- sowing seed treatment-a shotgun approach to improve germination growth and crop yield under saline and none-saline conditions. Adv Agron 88:223–271CrossRefGoogle Scholar
  5. Baskin CC, Baskin JM (1991) Non-deep complex morphophysiological dormancy in seeds of Osmorhiza claytonia (Apiaceae). Am J Bot 78:588–593CrossRefGoogle Scholar
  6. Basnet DB (2001) Evolving nursery practices and method of cultivation of high value medicinal plant Swertia chirayita ham. Environ Ecol 19:935–938Google Scholar
  7. Basra SMA, Zia MN, Mehmood T, Afzal I, Khaliq A (2002) Comparison of different invigoration techniques in wheat seeds. Pakistan J Arid Agric 5:11–16Google Scholar
  8. Bewley JD, Black M (1994) Seeds: physiology of development and germination. Plenum, New York, pp 65–87CrossRefGoogle Scholar
  9. Bhattacharjee SK (2001) Handbook of medicinal plants, vol 1. Pointer Publisher, Jaipur, pp 69–71Google Scholar
  10. Chakraborty S, Mukherjee D (2010) A text book of practical approaches in seed science and technology. New Delhi Publishers,
  11. Chakraborty S, Mukherjee D, Dasgupta T (2008) Cytological study on chromosome behaviour and new report on nature of mode of pollination of Swertia chirayita, a high value endangered medicinal plant of North Eastern Himalayan region. Caryologia 62(1):43–52Google Scholar
  12. Chakraborty S, Mukherjee D, Baskey S (2015a) Selection of lines of Valeriana jatamansi Jones, a high value medicinal plant in North Eastern Himalayan region. Indian J Genet Plant Breed 75(3):1–4Google Scholar
  13. Chakraborty S, Mukherjee D, Baskey S (2015b) Indian valerian, a highly endangered medicinal plant in north eastern Himalayan region. Adv Plants Agric Res 2(4):1–7Google Scholar
  14. Chakraborty S, Mukherjee D, Baskey S (2016) Morphological diversity and nomenclature of Swertia chirayita (Gentianaceae)- recovery of endangered medicinal plant population in north eastern Himalaya. Am J Plant Sci 7:741–755CrossRefGoogle Scholar
  15. Choudhary DK, Kaul BL, Khan S (1996) Breaking seed dormancy of Podophyllum hexandrum Royle ex. Camb. (syn.P. emodi Wall.ex. Honigberger). J Non Timber For Prod 3:10–12Google Scholar
  16. Davies PJ (2004) Plant hormones: biosynthesis, signal transduction, action. Kluwer Academic Press, Dordrecht, pp 89–102Google Scholar
  17. Dezfuli PM, Sharif ZF, Janmohammadi M (2008) Influence of priming techniques on seed germination behavior of maize inbred lines (Zea mays L.). ARPN J Agric Biol Sci 3(3):22–25Google Scholar
  18. Du LV, Tuong TP (2002) Enhancing the performance of dry-seeded rice: effects of seed priming, seedling rate and time of seedling. In: Pandey S, Mortimer M, Wade L, Tuong TP, Lopes K, Hardy B (eds) Direct seeding: research strategies and opportunities. International Research Institute, Manila, pp 241–256Google Scholar
  19. Dubey NK, Kumar R, Tripathi P (2014) Global promotion of herbal medicine : India opportunity. Curr Sci 86:37–41Google Scholar
  20. El-Keblawy A, Al-Rawai A (2005) Effects of seed maturation time and dry storage on light and temperature requirements during germination in invasive Prosopis juliflora. Flora 201:135–143CrossRefGoogle Scholar
  21. Foti R, Abureni K, Tigere A, Gotosa J, Gerem J (2008) The efficacy of different seed priming osmotica on the establishment of maize (Zea mays L.) caryopses. J Arid Environ 72:1127–1130CrossRefGoogle Scholar
  22. Ghavami N, Ramin AA (2007) Salinity and temperature effect on seed germination of milk thistle. Commenwealth Soil Sci Plant Ann 38(20):2681–2691CrossRefGoogle Scholar
  23. Gupta RK (1987) Living himalaya: aspects of environment and resource ecology. Today & Tomorrow Printer & Publishers, New Delhi, pp 66–71Google Scholar
  24. International Seed Testing Association (ISTA) (1965) International rules for seed testing. Seed Sci Technol 13:300–513Google Scholar
  25. Jackson ML (1973) Soil chemical analysis. Prentice Hall of India Pvt. Ltd, New Delhi, pp 183–204Google Scholar
  26. Kala CP (2006) Medicinal plants of the high altitude cold desert in India, diversity, distribution and traditional use. Int J Biol Sci Manag 2:43–56Google Scholar
  27. Kandari LS, Rao KS, Chauhan K, Maikhuri RK, Purohit VK, Phondani PC, Saxena KG (2007) Effect of pre-sowing treatments on the seed germination of two endangered medicinal herbs of the himalaya (Anagelica gluca. Edgew and Pleurospermum aegelicoides (Wall. Ex.DC.) Benth.Ex C.B. Clarke). Proc Indian Natl Sci Acad 73(1):11–16Google Scholar
  28. Keil M, Hartle B, Guillaume A, Psiorz M (2000) Production of amarogentin in root cultures of Swertia chirayita. Planta Med 66:452–457CrossRefPubMedGoogle Scholar
  29. Khan HA, Ayub CM, Pervez MA, Bilal RM, Shahid MA, Ziaf K (2009) Effect of seed priming with NaCl on salinity tolerance of hot pepper (Capsicum annuum L.) at seedling stage. Soil Environment 28(1):81–87Google Scholar
  30. Kucera B, Cohn MA, Leubner MG (2005) Plant hormone interactions during seed dormancy release and germination. Seed Sci Res 15:281–307CrossRefGoogle Scholar
  31. McDonald MB (1999) Seed priming. Seed technology and its biological basis. In: Black M, Bewley JD (eds) . CRC press, Boca Raton, pp 287–316Google Scholar
  32. Mukherjee D (2008) Cultivation of Swertia chirayita : a high value medicinal herb in high altitude. J Med Aromat Plant Sci 30(4):350–355Google Scholar
  33. Mukherjee D (2009a) Medicinal plant in Darjeeling hills. In: Rai S, Moktan MW, Ali S (eds) Krishi Sandesh. Miazik International Volunteer Center, Japan, pp 118–121Google Scholar
  34. Mukherjee D (2009b) Current status, distribution and ethno-medicinal values of medicinal plant in hilly region of Darjeeling district of West Bengal. J Crop Weed 5(1):316–320Google Scholar
  35. Mukherjee D (2013a) Improved agricultural practices for high value medicinal plants under mid to high altitude situation. J Crop Weed 9(1):201–206Google Scholar
  36. Mukherjee D (2013b) Prospect and need of agro-forestry in the hills of Darjeeling. Indian Agric 57(1):21–27Google Scholar
  37. Mukherjee D (2014a) Medicinal plant with relation to biodiversity conservation at Darjeeling hill. In: Nehra, Gothwal, Ghosh (eds) Biodiversity in India: assessment, scope and conservation. Lap Lambert Academic Publishing, Deutschland, pp 43–73Google Scholar
  38. Mukherjee D (2014b) Effect of forest microhabitat on growth of high altitude plants in Darjeeling Himalaya. J Interacademicia 18(1):20–30Google Scholar
  39. Mukherjee D (2015) Swertia chirayita: endangered medicinal plant of temperate Himalaya. In: Hemantaranjan A (ed) Advances in plant physiology, vol 16. Scientific Publishers, Jodhpur, pp 336–356Google Scholar
  40. Mukherjee D (2016) Studies on ecology and outcome of various seed and organic input treatments on yield attribute and economic yield of Valeriana jatamansi. Himalayan Rev 1(2):193–200Google Scholar
  41. Mukherjee D, Chakraborty S, Roy A, Mokthan MW (2009) Differential approach of germplasm conservation of high value of medicinal plants in north eastern himalayan region. Int J Agric Environ Biotechnol 2(4):332–340Google Scholar
  42. Mukherjee D, Chakraborty S, Roy, A (2010) Innovative cultivation practices of critically endangered herb: Swertia chirayita, Publish from Directorate of Research, Uttar Banga Krishi Viswavidyalaya, Coochbehar. ISBN Number: 978-93-5137-910-2Google Scholar
  43. Mukherjee D, Chakraborty S, Baskey S (2015) Threatened medicinal plants biodiversity of Eastern Himalaya and its conservation. J Agric Technol 2(1):101–107Google Scholar
  44. Nautiyal BP, Prakash V, Chauhan RS, Purohit H, Nautiyal C (2001) An assessment of germinability, productivity and cost benefit analysis of Picrorhiza kurrooa, cultivated at lower altitude. Curr Sci 81:579–585Google Scholar
  45. Polunin O, Stainton A (1987) Concise flowers of the Himalaya. Oxford University Press, London, p 67Google Scholar
  46. Raina R, Johri AK, Srivastava LJ (1994) Seed germination studies in Swertia chirayita L. Seed Res 22:62–63Google Scholar
  47. Samant SS, Dhar U, Palni LMS (1998) Medicinal plants of Indian himalaya: diversity, distribution and potential values. Gyanodaya Prakashan, Nainital. 3, 163Google Scholar
  48. SAS Institute Inc (1988) SAS User’s guide. Statistical Analysis Institute, CaryGoogle Scholar
  49. Sedghi M, Gholipouri A, Seyedsharifi R (2008) Tocopherol accumulation and floral differentiation of medicinal pumpkin (Cucurbita pepo L.) in response to plant growth regulators. Not Bot Hort Agrobot Cluj 36(1):80–84Google Scholar
  50. Sfairi Y, Lahcen O, Feddy M, Nana AA (2012) Dormancy breaking and salinity/water stress effects on seed germination of Atlas cypress, an endemic and threatened coniferous species in Morocco. Afr J Biotechnol 11(19):4385–4390Google Scholar
  51. Shanmugavalli M, Renganayaki PR, Menaka C (2007) Seed dormancy and germination improvement treatments in fodder sorghum. Int Crops Res Inst Semi Arid Trop 3:1–3Google Scholar
  52. Sharma RK, Sharma S, Shanti SS (2005) Seed germination behavior of some medicinal plants of lahaul and spiti cold desert : implications for conservation and cultivation. Curr Sci 90:1113–1118Google Scholar
  53. Zheleznov AV, Solonenko LP, Zheleznova NB (1997) Seed protein of the wild and the cultivated Amaranthus species. Euphytica 39:77–182Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Dhiman Mukherjee
    • 1
  1. 1.Department of AgronomyBidhan Chandra Krishi ViswavidyalayaKalyani, NadiaIndia

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