Biphasic Treatment System for the Removal of Toxic and Hazardous Pollutants from Industrial Wastewaters

  • Ali HussainEmail author
  • Sumaira Aslam
  • Arshad Javid
  • Muhammad Rashid
  • Irshad Hussain
  • Javed Iqbal Qazi


Industrial effluents carrying diverse pollutants are discharged freely into the adjacent environments and percolate to the groundwater resources. Currently, the treatment strategies also consider recycling and reuse with the energy recovery. Novel approaches to remove these pollutants include biphasic systems. Different biphasic systems including liquid-liquid two-phase partitioning and solid-liquid partitioning systems have proved successful for the cleaning of effluents containing textile dyes, heavy metals, organic contaminants, pharmaceutical ingredients and many other xenobiotic compounds. The system efficacy is based on the careful selection of the phase-forming substance/polymer as well as control and maintenance of the operational parameters including temperature, pH and hydraulic retention time. Among the biological parameters, selection of the microbes either pure cultures or mixed cultures plays a very important role for the removal of xenobiotics in biphasic systems.


Aqueous biphasic system Biotreatment Hazardous wastes Remediation Wastewater treatment Xenobiotics 


  1. Aguilar O, Albiter V, Serrano-Carreón L, Rito-Palomares M (2006) Direct comparison between ion-exchange chromatography and aqueous two-phase processes for the partial purification of penicillin acylase produced by E. coli. J Chromatogr B 835(1–2):77–83CrossRefGoogle Scholar
  2. Albertsson P (1958) Partition of proteins in liquid polymer-polymer two phase systems. Nature 182:709–711CrossRefGoogle Scholar
  3. Almeida HFD, Freire MG, Marrucho IM (2016) Improved extraction of fluoroquinolones with recyclable ionic-liquid-based aqueous biphasic systems. Green Chem 18:2717–2725CrossRefGoogle Scholar
  4. Amsden BG, Bochanysz J, Daugulis AJ (2003) Degradation of xenobiotics in a partitioning bioreactor in which the partitioning phase is a polymer. Biotechnol Bioeng 84:399–405CrossRefGoogle Scholar
  5. Arriaga S, Muñoz R, Hernández S, Guieysse B, Revah S (2006) Gaseous hexane biodegradation by Fusarium solani in two liquid phase packed-bed and stirred tank bioreactors. Environ Sci Technol 40:2390–2395CrossRefGoogle Scholar
  6. Atkins P, de Paula J (2010) Atkins’ physical chemistry. Oxford University Press, New YorkGoogle Scholar
  7. Benavides J, Aguilar O, Lapizco-Encinas BH, Rito-Palomares M (2008) Extraction and purification of bioproducts and nanoparticles using aqueous two-phase systems strategies. Chem Eng Technol 31:838–845CrossRefGoogle Scholar
  8. Bhal A, Bhal BS, Tuli GD (2012) Essentials of physical chemistry. S. Chand, ChandigarhGoogle Scholar
  9. Bharagava RN, Chowdhary P, Saxena G (2017) Bioremediation an eco-sustainable green technology, its applications and limitations. In: Bharagava RN (ed) Environmental pollutants and their bioremediation approaches. CRC Press, Taylor & Francis Group, Boca Raton, pp 1–22CrossRefGoogle Scholar
  10. Bharagava RN, Mishra S (2017) Hexavalent chromium reduction potential of Cellulosimicrobium sp. isolated from common effluent treatment industries. Ecotoxicol Environ Saf 147:102–109CrossRefGoogle Scholar
  11. Bruce LJ, Daugulis AJ (1991) Solvent selection strategies for extractive biocatalysis. Biotechnol Prog 7:116–124CrossRefGoogle Scholar
  12. Cesario MT, Beeftink HH, Tramper J (1992) Biological treatment of waste gases containing poorly-water soluble compounds. In: Dragt AJ, van Ham J (eds) Biotechniques for air pollution abatements and odour control policies. Elsevier Science Publishers, Amsterdam, pp 135–140Google Scholar
  13. Chakraborty A, Sen K (2016) Impact of temperature and pH on phase diagrams of different aqueous biphasic systems. J Chromatogr A1433:41–55CrossRefGoogle Scholar
  14. Chandra R, Chowdhary P (2015) Properties of bacterial laccases and their application in bioremediation of industrial wastes. Environ Sci Process Impacts 17:326–342CrossRefGoogle Scholar
  15. Chakraborty A, Sen K (2017) Ionic liquid vs tri-block copolymer in a new aqueous biphasic system for extraction of Zn-cholesterol complex. J Mol Liq 229:278–284CrossRefGoogle Scholar
  16. Chowdhury S, Sidhwani IT (2009) Extraction of toxic metal ions using aqueous biphasic system. 13th Annual green chemistry and engineering conference, College Park, Maryland, June 23–25Google Scholar
  17. Chowdhary P, More N, Raj A, Bharagava RN (2017) Characterization and identification of bacterial pathogens from treated tannery wastewater. Microbiol Res Int 5(3):30–36CrossRefGoogle Scholar
  18. Collins LD, Daugulis AJ (1997) Biodegradation of phenol at high initial concentrations in two-phase partitioning batch and fed-batch bioreactors. Biotechnol Bioeng 55:155–162CrossRefGoogle Scholar
  19. Darnault C, Rockne K, Stevens A, Mansoori GA, Sturchio N (2005) Fate of environmental pollutants. Water Environ Res l77:2576–2658CrossRefGoogle Scholar
  20. Daugulis AJ (2001) Two-phase partitioning bioreactors: a new technology platform for destroying xenobiotics. Trends Biotechnol 19:457–462CrossRefGoogle Scholar
  21. Daugulis AJ, Amsden B, Bochanysz J, Kayssi A (2003) Delivery of benzene to Alcaligenes xylosoxidans by solid polymers in a two-phase partitioning bioreactor. Biotechnol Lett 25:1203–1207CrossRefGoogle Scholar
  22. Daugulis AJ, Boudreau NG (2008) Solid -liquid two phase partitioning bioreactors for the treatment of gas-phase volatile organic carbons by a microbial consortium. Biotechnol Lett 30:1583–1587CrossRefGoogle Scholar
  23. Daugulis AJ, Tomei MC, Guieysse B (2011) Overcoming substrate inhibition during biological treatment of mono-aromatics: recent advances in bioprocess design. Appl Microbiol Biotechnol 90:1589–1608CrossRefGoogle Scholar
  24. de Belval S, le Breton B, Huddleston J, Lyddiatt A (1998) Influence of temperature upon protein partitioning in poly(ethylene glycol)-salt aqueous two-phase systems close to the critical point with some observations relevant to the partitioning of particles. J Chromatogr B 711:19–29CrossRefGoogle Scholar
  25. de Souza RL, Campos VC, Ventura SPM, Soares CMF, Coutinho JAP, Lima AS (2014) Effect of ionic liquids as adjuvants on PEG–based ABS formation and the extraction of two probe dyes. Fluid Phase Equilib 375:30–36CrossRefGoogle Scholar
  26. Didi MA, Sekkal AR, Villemin D (2011) Cloud-point extraction of bismuth (III) with nonionic surfactants in aqueous solutions. Colloids Surf 375:169CrossRefGoogle Scholar
  27. Domínguez-Pérez M, Tomé LIN, Freire MG, Marrucho IM, Cabeza O, Coutinho JAP (2010) (Extraction of biomolecules using) aqueous biphasic systems formed by ionic liquids and amino acids. Sep Purif Technol 72:85–91CrossRefGoogle Scholar
  28. Ferreira AM, Coutinho JA, Fernandes AM, Freire MG (2014) Complete removal of textile dyes from aqueous media using ionic-liquid-based aqueous two-phase systems. Sep Purif Technol 128:58–66CrossRefGoogle Scholar
  29. Ferreira AM, Vania FM, Faustino MD, Coutinho JAP, Freire MG (2016) Improving the extraction and purification of immunoglobulin G by the use of ionic liquid as adjuvants in aqueous biphasic systems. J Biotechnol 236:166–175CrossRefGoogle Scholar
  30. Freire MG, Cláudio AFM, Araújo JMM, Coutinho JAP, Marrucho IM, Canongia Lopes JN, Rebelo LPN (2012) Aqueous biphasic systems: a boost brought about by using ionic liquids. Chem Soc Rev 41:4966–4995CrossRefGoogle Scholar
  31. Gao J, Chen L, Yan ZC (2014) Extraction of dimethyl sulfoxide using ionic-liquid-based aqueous biphasic systems. Sep Purif Technol 124:107–116CrossRefGoogle Scholar
  32. Goja MA, Yang H, Cui M, Li C (2014) Aqueous two-phase extraction advances for bioseparation. J Bioproces Biotechniq 4:1–8Google Scholar
  33. Govindarajalu K (2003) Industrial effluents and health status-a case study of Noyyal river basin. In: Proceedings of the 3rd international conference on environment and health. Chennai, India, pp 150–157Google Scholar
  34. Greve A, Kula MR (1991) Phase diagrams of new aqueous phase systems composed of aliphatic alcohols, salts and water. Fluid Phase Equilib 62:53–63CrossRefGoogle Scholar
  35. Hamzehzadeh H, Abbasi M (2015) The influence of 1-butyl-3-methyl-imidazolium bromide on the partitioning of L-tyrosine within the {polyethylene glycol 600 + potassium citrate} aqueous biphasic system at T = 298.15 K. J Chem Thermodyn 80:102–111CrossRefGoogle Scholar
  36. Hernández M, Quijano G, Muñoz R (2012) Key role of microbial characteristics on the performance of VOC biodegradation in two-liquid phase bioreactors. Environ Sci Technol 46:4059–4066CrossRefGoogle Scholar
  37. Hernández M, Quijano G, Thalasso F, Daugulis AJ, Villaverde S, Munoz R (2010) A comparative study of solid and liquid non-aqueous phases for the biodegradation of hexane in two-phase partitioning bioreactors. Biotechnol Bioeng 106:731–740CrossRefGoogle Scholar
  38. Iqbal M, Tao Y, Xie S, Zhu Y, Chen D, Wang X, Huang L, Peng D, Sattar A, Shabbir MAB, Hussain HI, Ahmed S, Yuan Z (2016) Aqueous two-phase system (ATPS): an overview and advances in its applications. Biol Proced Online 18:18CrossRefGoogle Scholar
  39. Isik M, Sponza D (2006) Biological treatment of acid dyeing wastewater using a sequential anaerobic/aerobic reactor system. Enzym Microb Technol 38:887–892CrossRefGoogle Scholar
  40. Ivetic DZ, Sciban MB, Vasic VM, Kukic DV, Prodanovic JM, Antov MG (2013) Evaluation of possibility of textile dye removal from wastewater by aqueous two-phase extraction. Desalin Water Treat 51:1603–1608CrossRefGoogle Scholar
  41. Janikowski TB, Velicogna D, Punt M, Daugulis AJ (2002) Use of a two-phase partitioning bioreactor for degrading polycyclic aromatic hydrocarbons by a Sphingomonas sp. Appl Microbiol Biotechnol 59:368–376CrossRefGoogle Scholar
  42. Jhung JK, Choi E (1995) A comparative study of UASB and anaerobic fixed film reactor in the development of sludge granulation. Water Res 29:271–277CrossRefGoogle Scholar
  43. Jimena MG, Roxana O, Catiana Z, Margarita H, Susana M, Ines-Isla M (2008) Industrial effluents and surface waters genotoxicity and mutagenicity evaluation of a river of Tucuman, Argentina. J Hazard Mater 155:403–406CrossRefGoogle Scholar
  44. Kalaivani S, Xiuyun Ye IR, Yoshida S, Ng TB (2015) Synergistic extraction of a-Lactalbumin and b-Lactoglobulin from acid whey using aqueous biphasic system: process evaluation and optimization. Sep Purif Technol 146:301–310CrossRefGoogle Scholar
  45. Lin YK, Ooi CW, Tan JS, Show PL, Ariff A, Ling TC (2013) Recovery of human interferon α-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems. Sep Purif Technol 120:362–366CrossRefGoogle Scholar
  46. Liu X, Gao Y, Tang R, Wang W (2006) On the extraction and separation of iodide complex of cadmium (II) in propyl-alcohol ammonium sulfate aqueous biphasic system. Sep Purif Technol 50:263–266CrossRefGoogle Scholar
  47. Machado FLC, Coimbra JSDR, Zuniga ADG, da Costa AR, Martins JP (2012) Equilibrium data of aqueous two-phase systems composed of poly(ethylene glycol) and maltodextrin. J Chem Eng Data 57:1984–1990CrossRefGoogle Scholar
  48. Marcoux J, Deziel E, Villemur R, Lepine F, Bisaillon JG, Beaudet R (2000) Optimization of high-molecular-weight polycyclic aromatic hydrocarbons’ degradation in a two-liquid-phase bioreactor. J Appl Microbiol 88:655–662CrossRefGoogle Scholar
  49. Morrish JLE, Daugulis AJ (2008) Improved reactor performance and operability in the biotransformation of carveol to carvone using a solid–liquid two-phase partitioning bioreactor. Biotechnol Bioeng 101:946–956CrossRefGoogle Scholar
  50. Naganagouda K, Mulimani VH (2008) Aqueous two-phase extraction (ATPE): an attractive and economically viable technology for downstream processing of Aspergillus oryzae α-galactosidase. Process Biochem 43(11):1293–1299CrossRefGoogle Scholar
  51. Ogunfowokan AO, Okoh EK, Adenuga AA, Asubiojo OI (2005) An assessment of the impact of point source pollution from a university sewage treatment oxidation pond on a receiving stream – a preliminary study. J Appl Sci 5:36–43CrossRefGoogle Scholar
  52. Ooi CW, Tey BT, Hii SL, Mazlina S, Kamal M, Lan JCW, Ariff A, Ling TC (2009) Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems. Process Biochem 44:1083–1087CrossRefGoogle Scholar
  53. Paik SP, Sen K (2016) Species dependent iodine extractions in polymer based aqueous biphasic systems: emerging relations with aggregation number of polymeric micelles. J Mol Liq 223:1062–1066CrossRefGoogle Scholar
  54. Patrício PR, Cunha RC, Rodriguez Vargas SJ, Coelho YL, Mendes da Silva LH, Hespanhol da Silva MC (2016) Chromium speciation using aqueous biphasic systems: development and mechanistic aspects. Sep Purif Technol 158:144–154CrossRefGoogle Scholar
  55. Pei Y, Wang J, Xuan X, Fan J, Fan M (2007) Factors affecting ionic liquids based removal of anionic dyes from water. Environ Sci Technol 41:5090–5095CrossRefGoogle Scholar
  56. Pereira JFB, Lima ÁS, Freire MG, Coutinho JAP (2010) Ionic liquids as adjuvants for the tailored extraction of biomolecules in aqueous biphasic systems. Green Chem 12:1661–1669CrossRefGoogle Scholar
  57. Pereira JFB, Ventura SPM, e Silva FA, Shahriari S, Freire MG, JAP C (2013) Aqueous biphasic systems composed of ionic liquids and polymers: a platform for the purification of biomolecules. Sep Purif Technol 113:83–89CrossRefGoogle Scholar
  58. Pereira MM, Pedro SN, Quental MV, Lima ÁS, Coutinho JAP, Freire MG (2015) Enhanced extraction of bovine serum albumin with aqueous biphasic systems of phosphonium- and ammonium-based ionic liquids. J Biotechnol 206:17–25CrossRefGoogle Scholar
  59. Pratiwi AI, Yokouchi T, Matsumoto M, Kondo K (2015) Extraction of succinic acid by aqueous two-phase system using alcohols/salts and ionic liquids/salts. Sep Purif Technol 155:127–132CrossRefGoogle Scholar
  60. Prpich GP, Daugulis AJ (2004) Polymer development for enhanced delivery of phenol in a solid-liquid two-phase partitioning bioreactor. Biotechnol Prog 20:1725–1732CrossRefGoogle Scholar
  61. Prpich GP, Rehmann L, Daugulis AJ (2008) On the use, and re-use, of polymers for the treatment of hydrocarbon contaminated water via a solid-liquid partitioning bioreactor. Biotechnol Prog 24:839–844CrossRefGoogle Scholar
  62. Quijano G, Hernández M, Thalasso F, Muñoz R, Villaverde S (2009) Two-phase partitioning bioreactors in environmental biotechnology. App Microbiol Biotechnol 84:829–846CrossRefGoogle Scholar
  63. Quijano G, Hernández M, Villaverde S, Thalasso F, Muñoz R (2010) A step-forward in the characterization and potential applications of solid and liquid oxygen transfer vectors. Appl Microbiol Biotechnol 85:543–551CrossRefGoogle Scholar
  64. Raja S, Murty RM, Thivaharan V, Rajasekar V, Ramesh V (2011) Aqueous two phase systems for the recovery of biomolecules-a review. Sci Technol 1(1):7–16CrossRefGoogle Scholar
  65. Rajaram T, Ashutost D (2008) Water pollution by industrial effluents in India: discharge scenario and case for participatory ecosystem specific local regulation. Environment J40:56–69Google Scholar
  66. Ramos JL, Duque E, Gallegos MT, Godoy P, Ramos-Gonzalez MI, Rojas A, Teran W, Segura A (2002) Mechanisms of solvent tolerance in gram-negative bacteria. Annu Rev Microbiol 56:743–768CrossRefGoogle Scholar
  67. Rehmann L, Prpich GP, Daugulis AJ (2008) Remediation of PAH contaminated soils: application of a solid–liquid two-phase partitioning bioreactor. Chemosphere 73:798–804CrossRefGoogle Scholar
  68. Rehmann L, Sun B, Daugulis AJ (2007) Polymer selection for biphenyl degradation in a solid-liquid two-phase partitioning bioreactor. Biotechnol Prog 23:814–819CrossRefGoogle Scholar
  69. Rocha-Rios J, Quijano G, Thalasso F, Revah S, Muñoz R (2011) Methane biodegradation in a two-phase partition internal loop airlift reactor with gas recirculation. J Chem Technol Biotechnol 86:353–360CrossRefGoogle Scholar
  70. Rodrigues CD, de Lemos LR, da Silva MCH, da Silva LH (2013) Application of hydrophobic extractant in aqueous two phase systems for selective extraction of cobalt, nickel and cadmium. J Chromatogr A 1279:13–19CrossRefGoogle Scholar
  71. Rodriguez ER, Richardson JB, Ghosh S (1998) Removal of heavy metals and pathogens during biphasic fermentation of solid wastes. In: Proceedings of the conference on hazardous waste research. Salt Lake City, UT, pp 363–373Google Scholar
  72. Rogers RD (1995) Metal ion separations in polyethylene glycol-based aqueous biphasic systems. 6th Conference separation of ionic solutes, Slovakia, May 15–19CrossRefGoogle Scholar
  73. Sadeghi R, Maali M (2016) Toward an understanding of aqueous biphasic formation in polymer-polymer aqueous systems. Polymer 83:1–11CrossRefGoogle Scholar
  74. Safonova EA, Mehling T, Storm S, Ritter E, Smirnova IV (2014) Partitioning equilibria in multicomponent surfactant systems for design of surfactant based extraction processes. Chem Eng Res Des 92:2840–2850CrossRefGoogle Scholar
  75. Santos JHPM, Silva FAE, Couinho JAP, Ventura SPM, Pessoa A (2015) Ionic liquids as a novel class of electrolytes in polymeric aqueous biphasic systems. Process Biochem 50:661–668CrossRefGoogle Scholar
  76. Sen K, Chakraborty A (2016) A glycine based aqueous biphasic system: application in sequential separation of Ni, Cu and Zn. J Mol Liq 218:106–111CrossRefGoogle Scholar
  77. Senthilkumar M, Gnanapragasam G, Arutchelvan V, Nagarajan S (2011) Influence of hydraulic retention time in a two-phase up flow anaerobic sludge blanket reactor treating textile dyeing effluent using sago effluent as the co-substrate. Environ Sci Pollut Res 18:649–654CrossRefGoogle Scholar
  78. Seth R, Goyal SK, Handa BK (1995) Fixed film biomethanation of distillery spentwash using low cost porous media. Resour Conserv Recy 14:79–89CrossRefGoogle Scholar
  79. Shahriari S, Tome LC, Araújo JMM, Rebelo LPN, Coutinho JAP, Marrucho IM, Freire MG (2013) Aqueous biphasic systems: a benign route using cholinium-based ionic liquids. RSC Adv 3:1835–1843CrossRefGoogle Scholar
  80. Sheikhian L, Akhond M, Absalan G (2014) Partitioning of reactive red-120, 4-(2-pyridylazo)-resorcinol, and methyl orange in ionic liquid-based aqueous biphasic systems. J Environ Chem Eng 2:137–142CrossRefGoogle Scholar
  81. Silva ME, Franco TT (2000) Liquid-liquid extraction of biomolecules in downstream processing–a review paper. Braz J Chem Eng 17(1):1–17CrossRefGoogle Scholar
  82. Silva MSC, Sentos-Ebinuma VC, Lopes AM, Rangel-Yagui CO (2015) Dextran sulfate/triton X two phase micellar systems as an alternative first purification step for clavulanic acid. Fluid Phase Equilib 399:80–86CrossRefGoogle Scholar
  83. Souza RL, Lima RA, Coutinho JAP, Soares CMF, Lima AS (2015) Novel aqueous two phase systems based on tetrahydrofuran and potassium phosphate buffer for purification of lipase. Process Biochem 50:1459–1467CrossRefGoogle Scholar
  84. Taghavivand M, Pazuki G (2014) A new biocompatible gentle aqueous biphasic system in cefalexin partitioning containing nonionic Tween 20 surfactant and three organic/inorganic different salts. Fluid Phase Equilib 379:62–71CrossRefGoogle Scholar
  85. Talarposhti AM, Donnelly T, Andersonm GK (2001) Colour removal from a simulated dye wastewater using a two-phase anaerobic packed bed reactor. Water Res 35(2):425–432CrossRefGoogle Scholar
  86. Tan GYT, Zimmermann W, Lee K-H, Lan JC-W, Yim HS, Ng HS (2017) Recovery of mangostins from Garcinia mangostana peels with an aqueous micellar biphasic system. Food Bioprod Process 102:233–240CrossRefGoogle Scholar
  87. Thompi J (2000) Studies in effluent treatment. PhD thesis, Mumbai University, IndiaGoogle Scholar
  88. Tomei MC, Mosca Angelucci D, Daugulis AJ (2016) Towards a continuous two-phase portioning bioreactor for xenobiotic removal. J Hazard Mater 317:403–415CrossRefGoogle Scholar
  89. Tomei MC, Rita S, Mosca Angelucci D, Annesini MC, Daugulis AJ (2011) Treatment of substituted phenol mixtures in single phase and two-phase solid-liquid partitioning bioreactors. J Hazard Mater 191:190–195CrossRefGoogle Scholar
  90. Villemur R, dos Santos SCC, Ouellette J, Juteau P, Lépine F, Déziel E (2013) Biodegradation of endocrine disruptors in solid-liquid two-phase partitioning systems by enrichment cultures. Appl Environ Microbiol 79:4701–4711CrossRefGoogle Scholar
  91. Wedler G (1997) Lehrbuch der physikalischen chemie. Wiley-VCH, WeinheimGoogle Scholar
  92. Whiting SN, de Souza MP, Terry N (2001) Rhizosphere bacteria mobilize Zn for hyperaccumulation by Thlaspi caerulescens. Environ Sci Technol 35:3144–3150CrossRefGoogle Scholar
  93. Wilms DA, Van Haute AA (1984) Primary flocculation of wastewater with Al2(SO4)3 and NaAlO2 salts recuperated from spent aluminium anodising baths. Stud Environ Sci 23:213–220CrossRefGoogle Scholar
  94. Yadav S (2012) Degradation and decolourisation of post methanated distillery effluent in biphasic treatment system of bacteria and wetland plant for environmental safety. PhD thesis, Pandit Ravi Shankar Shula University, IndiaGoogle Scholar
  95. Yazbik V, Ansorge-Schumacher M (2010) Fast and efficient purification of chloroperoxidase from C. fumago. Process Biochem 45(2):279–283CrossRefGoogle Scholar
  96. Yeom SH, Dalm MCF, Daugulis AJ (2000) Treatment of high concentration gaseous benzene streams using a novel bioreactor system. Biotechnol Lett 22:1747–1751CrossRefGoogle Scholar
  97. Zhou XY, Zhang J, Xu RP, Ma X, Zhang ZQ (2014) Aqueous biphasic systems based on low-molecular-weight polyethylene glycol for one-step separation of crude polysaccharides from Pericarpium granati using high speed countercurrent chromatography. J Chromatogr A1362:129–134CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Ali Hussain
    • 1
    Email author
  • Sumaira Aslam
    • 2
  • Arshad Javid
    • 1
  • Muhammad Rashid
    • 3
  • Irshad Hussain
    • 3
  • Javed Iqbal Qazi
    • 4
  1. 1.Applied and Environmental Microbiology Laboratory, Department of Wildlife and EcologyUniversity of Veterinary and Animal SciencesLahorePakistan
  2. 2.Microbiology and Biotechnology Laboratory, Department of ZoologyGovernment College Women UniversityFaisalabadPakistan
  3. 3.General Chemistry Laboratory, Faculty of Fisheries and WildlifeUniversity of Veterinary and Animal SciencesLahorePakistan
  4. 4.Microbial Biotechnology Laboratory, Department of ZoologyUniversity of the PunjabLahorePakistan

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