Generation of Aptamers Against Natural Toxins and Their Application as Biosensors

Chapter

Abstract

RNA or ssDNA aptamers, which are capable of binding to target molecules with high affinity and specificity, are selected in vitro from large combinatorial nucleic acid libraries by a process known as systematic evolution of ligands by exponential enrichment (SELEX). Using this SELEX technology, many aptamers have been generated against a wide range of target molecules, including proteins, nucleic acids, small molecules, and whole cells. In this chapter, we described various methods for generating aptamers, including methods that do not require target immobilization. Among these aptamers, ones that are specific for natural toxins, such as mycotoxins, are of great interest to the food industry, as they can used in developing tools (biosensors) for ensuring food safety. We also summarized several aptamer-based detection strategies. Lastly, we described biosensor applications of aptamers for natural toxins.

Keywords

RNA Aptamer SELEX Aptasensor Natural toxin 

Notes

Acknowledgments

We thank Dr. Yasuyuki Tomita for helpful discussions.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Research Laboratories for Health Science & Food Technologies, Research and Development DivisionKirin Company LimitedYokohamaJapan
  2. 2.Central Laboratories for Key Technologies, Research and Development DivisionKirin Company LimitedYokohamaJapan

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