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Haematoxylin and Eosin Stain of the Tissue Section

  • Pranab Dey
Chapter

Abstract

Haematoxylin is the most common routinely used dye in the histopathology. It stains the nuclei, and in combination with the cytoplasmic stain eosin, it provides good contrast. Haematoxylin also stains collagenous material, minerals and myelin fibres. Haematoxylin is oxidized to haematein which is a weak anionic dye. When a metallic salt (mordant) is combined with haematein, then a cationic dye-metal complex is formed that behaves as strong basic dye and combines with nucleic acid. This chapter discusses the different types of haematoxylin solutions and their uses along with the staining procedure.

Keywords

Haematoxylin Stain Eosin Mordant Haematein Bluing Mayer’s haematoxylin Harris haematoxylin Tungsten haematoxylin Verhoeff’s iron haematoxylin Heidenhain’s iron haematoxylin Ehrlich’s haematoxylin 

References

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    King DF, King LA. A brief historical note on staining by hematoxylin and eosin. Am J Dermatopathol. 1986;8:168.CrossRefPubMedGoogle Scholar
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    Brown PA. A review of technique used in the preparation, curation, and conservation of microscope slides at the natural history museum London. Biol Curator. 1997;10:1–33.Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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