Haematoxylin and Eosin Stain of the Tissue Section

  • Pranab Dey


Haematoxylin is the most common routinely used dye in the histopathology. It stains the nuclei, and in combination with the cytoplasmic stain eosin, it provides good contrast. Haematoxylin also stains collagenous material, minerals and myelin fibres. Haematoxylin is oxidized to haematein which is a weak anionic dye. When a metallic salt (mordant) is combined with haematein, then a cationic dye-metal complex is formed that behaves as strong basic dye and combines with nucleic acid. This chapter discusses the different types of haematoxylin solutions and their uses along with the staining procedure.


Haematoxylin Stain Eosin Mordant Haematein Bluing Mayer’s haematoxylin Harris haematoxylin Tungsten haematoxylin Verhoeff’s iron haematoxylin Heidenhain’s iron haematoxylin Ehrlich’s haematoxylin 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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