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Embedding of Tissue in Histopathology

  • Pranab Dey
Chapter

Abstract

This chapter discusses the embedding of the tissue in the medium to make a block for tissue sectioning and archival preservation of the representative tissue. The choice of the embedding medium of the tissue depends on type of tissue, type of microtomy and also type of microscope to examine the tissue. The various commonly used embedding media are discussed in this section along with the process of the embedding. Liquid paraffin is the most commonly used embedding medium in the histopathology laboratory. Nowadays various commercially available embedding systems are present in the market that provide liquid paraffin in a constant temperature along with a cold plate to cool the tissue block. Finally the orientation of the tissue in the embedding medium is discussed as this is a vital part to get useful information from the tissue.

Keywords

Embedding of tissue Embedding medium Paraffin wax Agar gel Tissue-Tek system 

References

  1. 1.
    Mccormick JB. Improved tissue-embedding method for paraffin and carbowax, using Tissue-Tek system. Tech Bull Regist Med Technol. 1959;29(1):15–6.PubMedGoogle Scholar
  2. 2.
    Dimenstein IB. Grossing biopsies: an introduction to general principles and techniques. Ann Diagn Pathol. 2009;13(2):106–13.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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