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Fluorescence and Confocal Microscope: Basic Principles and Applications in Pathology

  • Pranab Dey
Chapter

Abstract

Fluorescence microscope applies high-intensity light to illuminate the substance that emits fluorescence light. The present chapter describes the basic principle and applications of fluorescence microscopy. There are two types of fluorescence microscope: transmitted fluorescent microscope and incident fluorescent microscope. The working principles of both these types of microscopes are described. The confocal fluorescence microscopy (CFM) provides three-dimensional optical resolution. In CFM, at one time we see the image of the particular depth of the object at a small point. All the out-of-focus light is eliminated by passing the light through the pinhole. Multiple images at different depth are accumulated and then reconstructed to provide a three-dimensional image. The working principle and applications of CFM are discussed in the present chapter.

Keywords

Fluorescence microscope Fluorochrome dye Transmitted fluorescent microscope Incident fluorescent microscope Dichroic mirror Confocal microscopy Fluorescence recovery after photobleaching Green fluorescence protein 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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