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Stains for the Microbial Organisms

  • Pranab Dey
Chapter

Abstract

Microbial organisms are sub-microscopic infective organisms that usually produce diseases. They are subdivided into (1) bacteria, (2) fungi, (3) protozoa, (4) helminths and (5) virus. It is not always possible to identify the microbes by routine haematoxylin and eosin stain, and often special stains are required to demonstrate the morphology and characteristic colour of the organisms. This chapter describes the essential stains to demonstrate the various microorganisms in the histopathology and cytology material. The principle of stains, basic ingredients of the staining solution and the steps of staining have been described here.

Keywords

Microbial organisms Bacteria Fungi Protozoa Helminths Virus Ziehl-Neelsen stain Auramine-rhodamine stain Fite acid-fast stain Gomori methenamine silver Masson-Fontana silver stain 

References

  1. 1.
    Woods GL, Walker DH. Detection of infection or infectious agents by use of cytologic and histologic stains. Clin Microbiol Rev. 1996;9(3):382–404.PubMedPubMedCentralGoogle Scholar
  2. 2.
    Kwon-Chung KJ, Hill WB, Bennett JE. New, special stain for histopathological diagnosis of cryptococcosis. J Clin Microbiol. 1981;13(2):383–7.PubMedPubMedCentralGoogle Scholar
  3. 3.
    Madison BM. Application of stains in clinical microbiology. Biotech Histochem. 2001;76(3):119–25.CrossRefPubMedGoogle Scholar
  4. 4.
    Gram HC. Über die isolierte Färbung der Schizomyceten in Schnitt- und Trockenpräparaten. Fortschr Med. 1884;2:185–9.Google Scholar
  5. 5.
    Engbaek K, Johansen KS, Jensen ME. A new technique for Gram staining paraffin-embedded tissue. J Clin Pathol. 1979;32(2):187–90.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Wade HW. A modification of the Fite formaldehyde (Fite I) method for staining acid-fast bacilli in paraffin sections. Stain Technol. 1957;32(6):287–92.CrossRefPubMedGoogle Scholar
  7. 7.
    Grocott RG. A stain for fungi in tissue sections and smears using Gomori's methenamine-silver nitrate technique. Am J Clin Pathol. 1955;25:975–9.CrossRefPubMedGoogle Scholar
  8. 8.
    Warthin AS, Chronister AC. A more rapid and improved method of demonstrating spirochetes in tissues (Warthin and Starry’s cover-glass method). American Journal of Syphilis. 1920;4:97–103.Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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