Connective Tissue Stain: Principle and Procedure

  • Pranab Dey


Connective tissue is one of the major types of tissue that connects the different parts of tissue and also supports the body parts. The fibrous part of the connective tissue includes collagen, reticulin, elastin and basement membrane. There are predominantly five types of collagen fibres, and out of which, collagen I carries the main bulk of connective tissue. Reticulin fibres are actually collagen III fibre, and they support the parenchymal tissue of the liver, spleen and lymph node. The elastic fibres are made of microfibrils that are organized in complex pattern with the help of calcium. Elastic fibres provide the elasticity of the blood vessels, lung and skin. Basement membrane is the connective tissue elements that separate the epithelial and endothelial cells from the underlying connective tissue. This chapter discusses all these connective tissue fibres along with the different stains to elucidate them. The basic principles of these stains, preparation of the staining solutions, steps of the stain and final results have been described in detail.


Connective tissue Collagen fibres Reticulin fibres Elastic fibres Masson trichrome stain Van Gieson stain Reticulin stain Verhoeff’s stain Weigert’s resorcin-fuchsin stain Phosphotungstic acid haematoxylin stain 


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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Pranab Dey
    • 1
  1. 1.Education and Research (PGIMER)Post Graduate Institute of Medical Education and Research (PGIMER)ChandigarhIndia

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