To Perform Enzyme-Linked Immunosorbent Assay

  • Vijay Kumar
  • Kiran Dip Gill
Chapter

Abstract

Enzyme-linked immunosorbent assay (ELISA) is an immunoassay that combines the specificity of antibody and antigen usually detected in presence of enzyme conjugated with antibody. The enzyme linked with the antibody reacts with specific substrate to produce colored product which is related to antigen or antibody concentration.

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Vijay Kumar
    • 1
  • Kiran Dip Gill
    • 2
  1. 1.Department of BiochemistryMaharshi Dayanand UniversityRohtakIndia
  2. 2.Department of BiochemistryPostgraduate Institute of Medical Education & ResearchChandigarhIndia

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