Thidiazuron: An Effective Plant Growth Regulator on In Vitro Cloning of Slow-Growing Economic Rattan Palms in Southern Western Ghats for Eco-restoration and Consistent Utilization

  • T. S. Preetha
  • A. S. Hemanthakumar
  • S. Seeni


Rattans, the spiny climbing palms in Southeast Asia, are the fast depleting high-value non-wood forest produce primarily used for making furniture, baskets and handicraft items contributing significantly to the rural economy in the provinces where they occur. The most seriously affected species in the Western Ghats are Calamus thwaitesii, C. rotang, C. travancoricus, C. nagabettai, etc. which despites its wide distribution is indiscriminately exploited to the extent that mature useful canes are no more available. Since seed availability is scarce and traditional propagation methods are insufficient to meet the demand, in vitro cloning holds great promise for large-scale production and consistent utilization of planting material through eco-restoration activities. However, in vitro cloning of rattan palms is not thoroughly worked out, and there are only few isolated reports on micropropagation of rattan palms wherein the effectiveness of purine-based cytokinins such as BA or kinetin has been ruled out with very slow and little regeneration efficiency. The original work presented here reports the efficiency of thidiazuron (TDZ), a potent multidimensional cytokinin capable of fulfilling both the cytokinin and auxin requirements of various regeneration responses, viz. new meristem formation, and promotes multiple shoot induction from pre-existing meristems of elite rattan palms with special reference to C. rotang. TDZ has played a significant role to speed up inducing multiple shoot formation from offshoot/sucker-derived shoot tips with greater efficiency than other cytokinins (BA, 2-iP) in micro-cloning of rattan palms. The TDZ-induced in vitro multiplication scheme, developed for the first time in rattan palm tissue culture, can be gainfully employed for the focused production and supply of elite female rattan palms for open seeding and commercial plantation activities.


Rattan palm Calamus In vitro cloning Shoot tip Thidiazuron Reintroduction 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • T. S. Preetha
    • 1
  • A. S. Hemanthakumar
    • 2
  • S. Seeni
    • 2
  1. 1.Department of BotanyUniversity CollegeThiruvananthapuramIndia
  2. 2.Biotechnology and Bioinformatics DivisionJawaharlal Nehru Tropical Botanic Garden and Research InstitutePalode, ThiruvananthapuramIndia

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