Role of Thidiazuron in Tissue Culture of Orchids

  • Remya Mohanraj


Orchids are among the elite of flowering plants, fetching a very high price in the international market. The orchid industry of today is dependent on micropropagation for planting material, since orchid seeds lack a functional endosperm and do not germinate naturally without a fungal association. Proper growth and maintenance of orchid plantlets in tissue culture require an external supply of growth regulators. Recently, thidiazuron (TDZ) has surfaced as an effective bioregulant in plant cell and tissue cultures by virtue of its morpho-regulatory potential. It is reported to be the matchless synthetic cytokinin available and has significantly improved the in vitro regeneration and multiplication of species recalcitrant to propagation. This chapter summarizes the various developments and recent advancements in the application of TDZ for tissue culture of orchids.


Orchids Thidiazuron Micropropagation 


  1. Aung HN, Park IS, Hwang YJ, Chung JD, Lim KB (2010) In vitro micropropagation and conservation of Rhynchostylis retusa BL. Hortic Environ Biotechnol 51:440–444Google Scholar
  2. Balilashaki K, Ruhangiz N, Siamak K, Aboozar S (2014) Micropropagation of Phalaenopsis amabilis cv. Cool ‘Breeze’ with using of flower stalk nodes and leaves of sterile obtained from node cultures. Int J Farm Allied Sci 3:823–829Google Scholar
  3. Bastin M, Jeyachandran R (2015) Ex situ conservation of trichoglottis tenera (Lindl.) a threatened, and endangered orchid of western ghats using asymbiotic seed germination technique. Int J Recent Sci Res 6:3488–3496Google Scholar
  4. Bhattacharyya P, Suman K, Reemavareen D, Pramod T (2014) Genetic stability and phytochemical analysis of the in vitro regenerated plants of Dendrobium nobile Lindl., an endangered medicinal orchid. Meta Gene 2:489–504Google Scholar
  5. Deepak KS, Shashi BB (2016) In vitro propagation and chemical profiling of Herminium lanceum (Thunb. ex Sw.) Vuijk, a medicinally important orchid, for therapeutically important phenolic acids. Plant Biotechnol 33:153–160Google Scholar
  6. Dini M, Iriawati (2016) Regeneration of plantlets through PLB (protocorm-like body) formation in Phalaenopsis ‘Join Angle X Sogo Musadian’. J Math Fund Sci 48:204–212Google Scholar
  7. Gangadhar S, Mulgund K, Nataraja RB, Malabadi S, Kumar V (2011) TDZ induced in vitro propagation of an epiphytic orchid Xenikophyton smeeanum (Reichb. f.) Res Plant Biol 4:07–15Google Scholar
  8. Hajong S, Kumaria S, Tandon PJ (2013) Effect of plant growth regulators on regeneration potential of axenic nodal segments of Dendrobium chrysanthum wall. Ex Lindl Agr Sci Tech 15:1425–1435.
  9. Jitsopakul N, Kanchit T, Keiko I (2013) Efficient adventitious shoot regeneration from shoot tip culture of Vanda coerulea, a Thai orchid. Sci Asia 39:449–455Google Scholar
  10. Jones MP, Cao J, O’Brien R, Murch SJ, Saxena PK (2007) The mode of action of thidiazuron: auxins, indoleamines, and ion channels in the regeneration of Echinacea purpurea L. Plant Cell Rep 26:1481–1490Google Scholar
  11. Kou Y, Cunquan Y, Qingcui Z, Guoqin L, Jing N, Zhimin M, Chenxia C, Teixeira da Silva JA, Liangjun Z (2016) Thidiazuron triggers morphogenesis in Rosa canina L. Protocorm-like bodies by changing incipient cell fate. Front Plant Sci 7:557Google Scholar
  12. Latip MA, Rosmah M, Zaleha AA, Lai HT, Lilly MG, Rimi R (2010) Effects of N6 -Benzyladenine and Thidiazuron on proliferation of Phalaenopsis gigantea Protocorms. Asia Pac J Mol Biol Biotechnol 18:215–218Google Scholar
  13. Lee Y-I, Lee N (2003) Plant regeneration from protocorm-derived callus of Cypripedium Formosanum. In Vitro Cell Dev Biol Plant 39:475–479CrossRefGoogle Scholar
  14. Malabadi BR, Mulgund GS, Nataraja K (2004) Efficient Regeneration of Vanda coerulea, an Endangered Orchid Using Thidiazuron. Plant Cell Tissue Org Cult 76:289–293Google Scholar
  15. Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267–275CrossRefGoogle Scholar
  16. Natarajan K (2009) An in vitro study on micropropagation of Cymbidium orchids. Curr Biotica 3:244–250Google Scholar
  17. Nihar RN, Shiba PR, Satyanarayan P, Nihar RN, Shiba PR, Satyanarayan P (1997) In vitro propagation of three epiphytic orchids, Cymbidium aloifolium (L.) Sw., Dendrobium aphyllum (Roxb.) Fisch. and Dendrobium moschatum (Buch-Ham) Sw. through thidiazuron-induced high frequency shoot proliferation. Sci Hortic 71:243–250Google Scholar
  18. Panjan S, Kamnoon K (2011) Efficient direct protocorm-like bodies induction of dwarf Dendrobium using Thidiazuron. Not Sci Biol 3:88–92Google Scholar
  19. Rachmawati BW, Nurhajati AM, Ni Made AW, Agus P (2015) Shoot tips derived-somatic embryogenesis in mass propagation of Dendrobium Indonesia Raya ‘Ina’ Fitri. Emirates J Food Agric 27:1–10Google Scholar
  20. Samarfard S, Mihdzar AK, Saleh BK, Halimi MS, Seyed AR (2014) In vitro propagation and detection of somaclonal variation in Phalaenopsis gigantea as affected by chitosan and thidiazuron combinations. Hortscience 49:82–88Google Scholar
  21. Sebastinraj J, John Britto S, Vinoth Kumar D, Philip Robinson J, Thangavel P (2014) Rapid propagation of Vanda testacea (Lindl.) Rchb.F. – a highly medicinal value epiphytic orchid of India. World J Agric Sci 10:223–230Google Scholar
  22. Tan TK, Loon WS, Khor E, Loh CS (1998) Infection of Spathoglottis plicata (Orchidaceae) seeds by mycorrhizal fungus. Plant Cell Rep 18(1-2):14–19Google Scholar
  23. Tao Jun LYFK, Zhao D (2011) Effects of plant growth regulators on in vitro propagation of Cymbidium faberi Rolfe. Afr J Biotechnol 10:15639–15646Google Scholar
  24. Teixeira da Silva JA (2012) New basal media for protocorm-like body and callus induction of hybrid Cymbidium. J Fruit Ornamental Plant Res 20:127–133Google Scholar
  25. Utami ESW, Hariyanto S (2016) The effect of organic nutrient and growth regulators on seed germination, embryo and shoots development of Dendrobium antennatum Lindl. Orchid by in vitro. Biosaintifika J Biol Biol Educ 8:165–171Google Scholar
  26. Vacin EF, Went FW (1949) Some pH Changes in Nutrient Solutions. Bot Gaz 110(4):605–613Google Scholar

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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Remya Mohanraj
    • 1
  1. 1.Houston Community CollegeHoustonUSA

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